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Microbiology > Chapter 13 - Characterizing and Classifying Viruses, Viroids, and Prions > Flashcards

Chapter 13 - Characterizing and Classifying Viruses, Viroids, and Prions Flashcards

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Biology 101 flashcards
1
Q

What is a virus?

A
  • Miniscule, acellular, infectious agent having either DNA or RNA
  • Causes many infections of humans, animals, plants, and bacteria
  • Causes most of the diseases that plague the industrialized world

e.g. common cold, influenza, herpes, SARS, AIDS

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2
Q

List the characteristics of Viruses.

A
  • Cannot carry out any metabolic pathway
  • Neither grow nor respond to the environment
  • Cannot reproduce independently
  • Recruit the cell’s metabolic pathways to increase their numbers
  • No cytoplasmic membrane, cytosol, organelles
  • Have extracellular and intracellular state
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3
Q

What are the characteristics of an extracellular state?

A
  • Called virion
  • Protein coat (capsid) surrounding nucleic acid
  • Nucleic acid and capsid also called nucleocapsid
  • Some have phospholipid envelope
  • Outermost layer provides protection and recognition sites for host cells
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4
Q

What are the characteristics of an Intracellular state?

A
  • Capsid removed
  • Virus exists as nucleic acid
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5
Q

What is the genetic material of viruses?

A
  • Show more variety in nature of their genomes than do cells
  • Primary way scientists categorize and classify viruses
  • May be DNA or RNA, but never both
  • Can be dsDNA, ssDNA, dsRNA, ssRNA
  • May be linear and segmented or single and circular
  • Much smaller than genomes of cells
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6
Q

What types of hosts does a virus infect?

A
  • Most viruses infect only particular host’s cells
  • Due to affinity of viral surface proteins for complementary proteins on host cell surface
  • May be so specific they only infect particular kind of cell in a particular host e.g. HIV
  • Generalists – infect many kinds of cells in many different hosts including bacteria, archaea, protozoa, fungi, plants, animals and humans.

E.g. Influenza virus, West Nile virus

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7
Q

What are the sizes of viruses compared to other organisms?

A

Red Blood cell: 10,000nm in diameter

Bacterial ribosomes: 25nm

Poliovius: 30nm

Bacteriophage T4: (50nm x 225nm)

Smallpox virus : (200nm x 300nm)

Tabacco mosaic virus: ( 15nm x 300nm)

They are really small!

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8
Q

What is the history of viruses?

A

Ivanowski first demonstrated in 1892 that viruses are acellular

Conducted an experiment to prove acellularity
Stanley in 1935 isolated and characterized tobacco mosaic virus

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9
Q

What are the characteristics of Capsid Morphology?

A
  • Capsids:Protein coats that provide protection for viral nucleic acid and means of attachment to host’s cells
  • Composed of proteinaceous subunits called capsomeres
  • Capsomere may be made of single or multiple types of proteins
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10
Q

Name the shapes of virions.

A
  1. Helical (TMV)
  2. Polyhedral (common cold)
  3. Complex (smallpox virus and rabies virus)
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11
Q

What are enveloped viruses?

A
  • A virus with an outer envelope surrounding the capsid is an enveloped virion; virion without an envelope is called a non-enveloped or naked virion.
  • Matrix proteins fill the region between capsid and envelope.
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12
Q

How are viral envelopes acquired by a virus?

A
  • Acquired from host cell during viral replication or release
  • Envelope is portion of membrane system of host
  • Composed of phospholipid bilayer and proteins
  • Some proteins are virally coded glycoproteins (spikes)
  • Envelope’s proteins and glycoproteins often play role in host recognition
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13
Q

What are the classification of viruses?

A

Classified based on the type of nucleic acid, presence of envelope, shape and size

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14
Q

What are the characteristics of Viral Replication?

A

Dependent on hosts’ organelles and enzymes to produce new virions

Lytic replication

Replication cycle usually results in death and lysis of host cell

Stages of lytic replication cycle
Attachment
Entry
Synthesis
Assembly
Release

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15
Q

Describe the lytic cycle in a bacteriophage

A
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16
Q

What is burst time?

A

Period of time required to complete the entire process of synthesis, assembly and release

17
Q

What is Burst size?

A

The number of new virions released per lysed host cell

18
Q

What is lysogeny and describe the lysogenic replication cycle.

A

Lysogeny

  • Modified replication cycle
  • Infected host cells grow and reproduce normally for generations before they lyse
  • Temperate phages or lysogenic phages
  • Prophages – inactive phages
  • Lysogenic conversion results when phages carry genes that alter phenotype of a bacterium
19
Q

What are some of the characteristics of animal viruses?

A
  • Same basic replication pathway as bacteriophages
  • Differences result from
  • Presence of envelope around some viruses
  • Eukaryotic nature of animal cells
  • Lack of cell wall in animal cells
20
Q

How do animal viruses attach to the host cell?

A
  • Attachment of animal viruses
  • Chemical attraction
  • Animal viruses do not have tails or tail fibers
  • Have glycoprotein spikes or other attachment molecules that mediate attachment
21
Q

What are the three mechanisms of entry of animal viruses?

A
  1. Direct penetration
  2. Membrane Fusion
  3. Endocytosis
22
Q

How are animal viruses synthesized?

A
  • Synthesis of animal viruses
  • Each type of animal virus requires different strategy depending on its nucleic acid
  • DNA viruses often enter the nucleus, except poxviruses
  • RNA viruses often replicate in the cytoplasm

Must consider:

  • How mRNA is synthesized
  • What serves as template for nucleic acid replication
23
Q

How does the replication of dsDNA viruses occur?

A

Similar to normal replication of cellular DNA and translation of proteins.

dsDNA -mRNA - viral proteins

24
Q

How does the replication of ssDNA viruses occur?

A

A new strand of complementary DNA is made which then binds to the ssDNA of the virus to form a dsDNA molecule. Replication then proceeds like dsDNA virus.

ssDNA - dsDNA - mRNA - viral proteins

25
Q

How does a retrovirus replicate?

A

Positive sense RNA viruses
Do not use their genome as mRNA
Synthesize a DNA intermediary from +RNA by reverse transcriptase enzyme
DNA intermediary then serves as template for synthesis of additional +RNA molecules which acts as mRNA for protein synthesis.
e.g. of retrovirus- HIV

+ssRNA - DNA - +ssRNA (acts as mRNA) - viral proteins

26
Q

How are animal virsuses assembled and released?

A
  • Most DNA viruses assemble in nucleus
  • Most RNA viruses develop solely in cytoplasm
  • Number of viruses produced depends on type of virus and size and initial health of host cell
  • Enveloped viruses cause persistent infections and released via budding
  • Naked viruses are released by exocytosis or lysis
27
Q

What is meant by the latency of animal viruses?

A
  • When animal viruses remain dormant in host cells e.g. chickenpox virus, herpes virus
  • May be prolonged for years with no viral activity
  • Some latent viruses do not become incorporated into host chromosome
  • Incorporation of provirus into host DNA is permanent
28
Q

How are enveloped viruses released?

A

Released through budding

Viral glycoproteins found on cell membrane, capsid fuses with the cytoplasmic membrane, cell does not lyse

results in continuous release of virus

29
Q

How are viuses cultured?

A

There are three types of media:

  1. Mature organims (bacteria, plants, or animals)
  2. Embryonated (fertilized) eggs
  3. Cell cultures
30
Q

What are the characteristics of culturing cells in cell (tissue) culture?

A
  • Consists of cells isolated from an organism and grown on a medium or in a broth

Two types of cell cultures:

1.Diploid cell cultures: Created from embryonic plant, animal or human, Do not last more than 100 generations

  1. Continuous cell cultures
    - Longer lasting
    - Derived from tumor cells
    E.g. HeLa cell line (Henrietta Lacks)
31
Q

What are the characteristics of Viroids?

A
  • Extremely small, circular pieces of RNA that are infectious and pathogenic in plants
  • Similar to RNA viruses, but lack capsid
  • May appear linear due to hydrogen bonding within the molecule
  • Several plant diseases are caused by viroids
32
Q

What are the characteristics of Prions?

A
  • Proteinaceous infectious agents first described by Stanley Prusiner in 1982
  • Lack nucleic acid
33
Q

What are the diseases associated with Prions?

A

Prion diseases:

  • Fatal neurological degeneration, fibril deposits in brain, and loss of brain matter
  • Large vacuoles form in brain
  • Characteristic spongy appearance
  • Spongiform encephalopathies – BSE, vCJD, kuru
  • Prions only destroyed by incineration or autoclaving in 1 N NaOH
34
Q

How does a prion cause disease?

A
  • Cellular PrP protein (a cytoplasmic membrane protein which plays a role in normal activity of brain)

Made by all mammals

                       Normal structure with B-helices called cellular PrP
  • Prion PrP: Disease-causing form with B-sheets called prion PrP
  • Prion PrP converts cellular PrP into prion PrP by inducing conformational change

Microbiology (6 decks)

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