Chapter 13

Question 1

Which of the following is a type of RNA that gets translated?

a. rRNA
b. mRNA
c. tRNA
d. miRNA
e. A, B, and C all get translated.

Answer 1

b

Question 2

Which of the following RNA molecules are required for the process of translation?

a. crRNA
b. tRNA
c. snRNA
d. snoRNA
e. siRNA

Answer 2

b

Question 3

Over time, DNA replaced RNA as the primary carrier of genetic information, and the chemical stability of DNA is believed to be the key reason for this. Which attribute of DNA is the reason behind its chemical stability?

a. DNA lacks a free hydroxyl group on the 2′-carbon atom of its sugar.
b. Unlike RNA, DNA is usually double-stranded.
c. DNA does not usually form hairpin loops.
d. One of the two pyrimidines found in DNA does not involve uracil.
e. DNA contains thymines, which make it more chemically stable.

Answer 3

a

Question 4

This molecule is synthesized using nucleotides containing the bases adenine, guanine, cytosine, and uracil.

a. RNA only
b. DNA only
c. Both RNA and DNA
d. Neither RNA nor DNA

Answer 4

a

Question 5

In eukaryotes, tRNAs are

a. transcribed in the nucleus and function in the nucleus.
b. transcribed in the nucleus but function in the cytoplasm.
c. transcribed in the cytoplasm and function in the cytoplasm.
d. transcribed in both the nucleus and the cytoplasm and function in the cytoplasm.
e. transcribed in the cytoplasm and function in the nucleus.

Answer 5

b

Question 6

____________________ probably began the evolution of life on Earth.

a. DNA
b. RNA promoters
c. DNA polymerases
d. RNA polymerases
e. Ribozymes

Answer 6

e

Question 7

During transcription, which parts of a DNA molecule are transcribed into RNA?

a. All of the nucleotides in DNA on both strands
b. All of the nucleotides on one strand of DNA
c. Only parts of the DNA that encode mRNA
d. Only regions of the DNA that contain genes
e. Only regions of the DNA that encode rRNA

Answer 7

d

Question 8

Which of the following statements are TRUE regarding transcription in most organisms?

a. All genes are transcribed from the same strand of DNA.
b. Both DNA strands are used to transcribe a single gene.
c. Different genes may be transcribed from different strands of DNA.
d. The DNA template strand is used to encode double stranded RNA.
e. The DNA nontemplate strand is used to encode single stranded RNA.

Answer 8

c

Question 9

Which of the following is a sequence of DNA where transcription is initiated?

a. Hairpin loop
b. TBP
c. Initiator
d. Sigma factor
e. Promoter

Answer 9

e

Question 10

Where are promoters usually located?

a. Upstream of the start site
b. Downstream of the start site
c. Near nucleotide +25
d. Near the hairpin loop
e. Downstream of the terminator

Answer 10

a

Question 11

Which of the following statements is NOT true?

a. Both DNA and RNA are synthesized in a 5′ to 3′ direction.
b. During RNA synthesis, the DNA template strand is read in a 3′ to 5′ direction.
c. During RNA synthesis, new nucleotides are added to the 3′ end of the growing RNA molecule.
d. RNA polymerase has 5′ to 3′ polymerase activity.
e. RNA molecules have the same 5′ to 3′ orientation as the DNA template strands to which they are complementary.

Answer 11

e

Question 12

Whereas the nucleotide strand used for transcription is termed the _______, the nontranscribed strand is called the _________.

a. promoter; terminator
b. terminator; promoter
c. transcription apparatus; TATA box
d. template strand; nontemplate strand
e. nontemplate strand; template strand

Answer 12

d

Question 13

In transcription, to which end of the elongating strand are nucleotides always added?

a. 3′
b. 5′
c. 3′ in prokaryotes and 5′ in eukaryotes
d. It depends on which RNA polymerase is being used.
e. It depends on which DNA strand is being used as the template.

Answer 13

a

Question 14

In a transcription reaction, two phosphate groups are cleaved from the incoming

a. deoxyribonucleoside diphosphate.
b. deoxyribonucleoside triphosphate.
c. ribonucleoside diphosphate.
d. ribonucleoside triphosphate.
e. ribozyme.

Answer 14

d

Question 15

What types of bonds are created between nucleotides during the process of transcription?

a. Ionic
b. Oxygen
c. Phosphodiester
d. Hydrogen
Both C and D

Answer 15

c

Question 16

In eukaryotes, which RNA polymerase transcribes the genes that encode proteins?

a. RNA polymerase I
b. RNA polymerase II
c. RNA polymerase III
d. RNA polymerase IV
e. RNA polymerase V

Answer 16

b

Question 17

The DNA replication enzyme that most closely resembles RNA polymerase is

a. DNA polymerase I.
b. DNA polymerase III.
c. primase.
d. telomerase.
e. helicase.

Answer 17

c

Question 18

When this molecule is synthesized, both strands of a DNA molecule are used as a template.

a. RNA only
b. DNA only
c. Both RNA and DNA
d. Neither RNA nor DNA

Answer 18

b

Question 19

This molecule is synthesized using triphosphate nucleotides as a substrate for a polymerase enzyme that forms phosphodiester bonds.

a. RNA only
b. DNA only
c. Both RNA and DNA
d. Neither RNA nor DNA

Answer 19

c

Question 20

The polymerase that synthesizes this molecule uses DNA as a template and synthesizes new strands from 5′ to 3′.

a. RNA only
b. DNA only
c. Both RNA and DNA
d. Neither RNA nor DNA

Answer 20

c

Question 21

This molecule is made of nucleotides joined by phosphodiester bonds that connect the 2′ OH to the 5′ phosphate.

a. RNA only
b. DNA only
c. Both RNA and DNA
d. Neither RNA nor DNA

Answer 21

d

Question 22

What is the function of eukaryotic RNA polymerase I?

a. Transcription of rRNA genes
b. Transcription of mRNA genes
c. Transcription of tRNA genes
d. Transcription of snRNAs
e. Initiation of transcription (but not elongation)

Answer 22

a

Question 23

Which of the following is NOT required for transcription?

a. Ribonucleotides
b. RNA primers
c. DNA template
d. RNA polymerase
e. Promoter

Answer 23

b

Question 24

Which process is illustrated in the diagram below?

a. Transcription
b. Translation
c. RNA processing
d. Replication
e. Nucleosome assembly

Answer 24

a

Question 25

Which statement about RNA polymerase is NOT true? a. RNA polymerase adds a ribonucleotide to the 3’ end of a growing RNA molecule. b. RNA polymerase binds to a promoter to initiate transcription. c. During transcription of a gene, RNA polymerase reads only one strand of DNA. d. RNA polymerase reads a template strand of DNA 5’ to 3’. e. RNA polymerase has many subunits.

Answer 25

d

Question 26

If the following DNA strand were used as a template, what would the sequence of an RNA be? 5′ GTACCGTC 3′ a. 5′ GUACCGUC 3′ b. 5′ GACGGTAC 3′ c. 5′ CAUGGCAG 3′ d. 5′ GACGGUAC 3′ e. 5′ GUCGGUAC 3′

Answer 26

d

Question 27

If the sequence of an RNA molecule is 5’-GGCAUCGACG-3’, what is the sequence of the nontemplate strand of DNA? a. 5’-GGCATCGACG-3’ b. 3’-GGCATCGACG-5’ c. 5’-CCGTAGCTGC-3’ d. 3’-CCGTAGCTGC-5’ e. 3’-CGTCGATGCC-5’

Answer 27

a

Question 28

Which of the following is NOT necessary for RNA polymerase to recognize the promoter of a bacterial gene? a. Sigma factor b. Origin of replication c. –10 consensus sequence d. –35 consensus sequence

Answer 28

b

Question 29

Which of the following features is primarily responsible for rho protein to cause termination of transcription? a. Recognizing unstructured RNA b. Helicase activity c. Migrating behind RNA polymerase d. RNA-binding activity e. Polymerase activity

Answer 29

b

Question 30

Prokaryotic promoters contain the sequence TATAAT at the position –10 from the transcription start. a. +1 b. –1 c. –10 d. –25 e. –35

Answer 30

c

Question 31

In prokaryotic RNA polymerases, the holoenzyme consists of the core enzyme and the a. rho factor. b. TFIID. c. TBP. d. omega subunit. e. sigma factor.

Answer 31

e

Question 32

When does sigma factor normally dissociate from RNA polymerase? a. After transcription has terminated b. After the process of initiation c. After the addition of nucleosomes d. After the binding of rho Following the addition of nucleosomes

Answer 32

b

Question 33

In prokaryotes, rho-independent transcription termination depends on a secondary structure formed in a. the RNA polymerase that is transcribing the gene. b. the DNA template. c. the RNA that is being transcribed. d. a protein factor that binds to RNA polymerase. a protein factor that binds to the RNA that is being transcribed

Answer 33

c

Question 34

An in vitro transcription system that contains a bacterial gene initiates transcription, but from random points on the DNA. Which of the following proteins most likely is missing from the reaction? a. Sigma factor b. Rho factor c. RNA polymerase II d. TATA-binding protein (TBP) e. TFIID

Answer 34

a

Question 35

An in vitro transcription system that contains a bacterial gene does not initiate transcription. What is one possible problem? a. Histones that were on the DNA when it was isolated from E. coli are blocking access to the template. b. There is a mutation in the inverted repeat sequence that prevents a hairpin secondary structure from forming. c. There is a mutation at –10, where a promoter consensus sequence is located. d. Rho factor has not been added. e. TATA-binding protein (TBP) has not been added.

Answer 35

c

Question 36

An in vitro transcription system transcribes a bacterial gene but terminates inefficiently. What is one possible problem? a. There is a mutation in the –10 consensus sequence, which is required for efficient termination. b. Rho factor has not been added. c. Sigma factor has not been added. d. A hairpin secondary structure has formed at the 3′ end of the mRNA, interfering with termination. e. Histones were added prematurely and interfered with termination.

Answer 36

b

Question 37

Which of the following statements is TRUE regarding the termination of transcription? a. In some organisms, transcription terminates thousands of nucleotides past the coding sequence. b. Transcription typically terminates precisely at the hairpin loop terminator sequence. c. In prokaryotes, transcription terminates as soon as rho has bound to the RNA. d. In yeast, transcription terminates as soon as Rat1 has bound to the RNA. e. Both C and D are correct answers.

Answer 37

a

Question 38

Which one of the following statements regarding eukaryotic transcription is NOT true? a. Eukaryotic transcription involves a core promoter and a regulatory promoter. b. There is no one generic promoter. c. A group of genes is transcribed into a polycistronic RNA. d. Chromatin remodeling is necessary before certain genes are transcribed. e. There are several different types of RNA polymerase.

Answer 38

c

Question 39

The TATA-binding protein (TBP) binds to the TATA box sequence in eukaryotic promoters. What is its function in transcriptional initiation? a. It blocks access of RNA polymerase to the promoter, until removed by general transcription factors. b. It is the subunit of prokaryotic RNA polymerase that is required to recognize promoters. c. It modifies histones so that nucleosomes can be removed from DNA for transcription. d. It bends and partly unwinds DNA at a promoter. e. It creates a phosphodiester bond between the nucleotides.

Answer 39

d

Question 40

In eukaryotic cells, where does the basal transcription apparatus bind? a. Core promoter b. Regulatory promoter c. Terminator d. Enhancer e. Ribozyme

Answer 40

a

Question 41

Which of the following statements is FALSE regarding TFIID? a. It contains a TATA binding protein. b. It aids in initiation of transcription. c. It binds to the core promoter. d. It binds to the TATA box. e. It is a transcriptional activator protein.

Answer 41

e

Question 42

What is the function of general transcription factors? a. They are DNA sequences to which RNA polymerase binds. b. They direct nucleosome assembly. c. They bind to regulatory promoters to increase the rate of transcription. d. They bind to enhancers to allow minimal levels of transcription. e. They are a part of the basal transcription apparatus.

Answer 42

e

Question 43

In which of the following organisms would transcription be the LEAST similar to archaea? a. E. coli b. Yeast c. Plants d. Mice e. Humans

Answer 43

a