Chapter 11 Flashcards
Cell membrane is composed of
Protein
Sugar
Lipid
Cholesterol
Which segment carries negative charge in a membrane phospholipid
Phosphate group
Where do lipids rapids rapidly diffuse in the bilayer?
Within own monolayer
Least common movement for lipids in bilayer
Flip flop
Which membrane would show a more rapid recovery of fluorescence in FRAP study?
Membrane with more unsaturated fat
How do organisms in cold climates adapt to low temp?
Keep membrane fluid by increasing unsaturated fatty acids in their membrane
When vesicle fuses with membrane, which way does the monolayer that was on the interior of the vesicle face
Cell exterior
Which way do hydrophobic side chains of a helices of a transmembrane protein face?
Outside membrane spanning helix
Detergent molecules are
Amphipathic
What determines cell shape and mechanical properties
Cell cortex
3 ways cells can restrict lateral movement
Diffusion barriers
Anchoring to internal components
Tethering to external components
How would a cell activate a redistribution of phospholipids that is rapidly transected to one monolayer?
Activating scramblase and inactivating flipase.
How is phosphatidylethanolamine found mainly in cytoplasmic monolayer
Specific flippers moves it to cytoplasmic monayer
Once a receptor is activated by signal molecule, where will the FRAP curve lie?
Significantly below baseline
What type of protein would ZP3 be to play role in cell recognition?
Glycoprotein
FRAP
Molecule is fluorescently labeled and then is relative mobility is measured
What procedure is used to analyze movement of transcriptional activations Mrs in living cells
Florescence recovery after photo bleaching FRAP
What would disrupt bilateral formation
Additional if phosphate to end of lipid tail
What procedure is used to track movement of individual protein in cell membrane?
Single particle tracking SPT
