Chapter 10

Question 1

Name 5 types of vectors

Answer 1

Plasmid, Phage, Fosmid, BAC, and YAC

Question 2

Describe vector plasmid

Answer 2

1 to 10 kb insert; high copy number e.g. pGLO

Question 3

Describe vector Phage

Answer 3

10-18 kb insert; protected in phage coat; high yield

Question 4

Describe vector Fosmid

Answer 4

35- 45 kb insert; single copy, so low or no recombination

Question 5

Describe vector BAC

Answer 5

100 – 200 kb insert; single copy

Question 6

Describe vector YAC

Answer 6

100 – 10,000 kb insert; single copy; grown in E. coli, then insert added and transformed into yeast.

Question 7

What two factors should you consider when choosing a vecor?

Answer 7

Desired size of insert and copy number of insert

Question 8

What are the 3 types of Gene libraries?

Answer 8

Genomic, Mitochondiral or chloroplast genomic, and cDNA.

Question 9

What is a Northern blot?

Answer 9

Shows the RNA on gel

Question 10

What is a southern blot?

Answer 10

shows DNA on gel

Question 11

What is a western blot?

Answer 11

shows protein on gel.