Chapter 1.0

Question 1

Who invented the compound microscope, and what were did it consist of ( magnification, parts)

Answer 1

The Janssen brothers
Hans and Zacherias

Composed of two convex lenses, started with a magnification of 20x.

Question 2

The first individual to observe cellular composition.

Answer 2

Robert Hooke

Question 3

The first individual to observe the movement of cells.

Answer 3

Anton Van Leeuwenhoek

Question 4

Who aided in part of the improvement of the miscroscope?

Answer 4

Anton Van Leeuwenhoek.

Question 5

List the magnifications if the low medium and high objective lenses.

Answer 5

4x, 10x, 40x.

Question 6

Formula of calculation of magnification.

Answer 6

(Ocular lense)(objective lense)

10x)(4,10,40x

Question 7

F.o.v.

Answer 7

Diameter of the slide, higher magnification, lower f.o.v.

Formula,
Fov1) (mag. 1) = (fov2)(mag. 2)

Question 8

1000 micrometre= ?

Answer 8

1mm

Question 9

Actual size calculation.

Answer 9

Fit number
* note they must be in the same units*

Question 10

Scale calculation.

Answer 10

Actual size

Question 11

The belief that living things came from non-living things is known as?

Answer 11

Spontaneous generation.

Question 12

Who is the father if biology, who often used the scientific method to explain things around him.

Answer 12

Aristotle

Question 13

Name those who tried to disprove spontaneous generation.

Answer 13

• Francessco Redi

- John Needhaum :attempted to relate it to living things.

Question 14

Who disproved spontaneous generation and when?

Answer 14

louis Pateur in the mid 1800s

Question 15

What is the cell theory.

Answer 15

All living things made of cells.
Cells a functional//
- meet all cell needs
- take in nutrients
- gives out waste 
- use energy to do work 
- maintains certain temperature and chemical conditions.
All existing cells  came from pre existing cells through mitosis ( cell division)

Question 16

Types of microscopes.

• em
• tem
• sem

Answer 16

Electron microscope
Transmission electron microscope
Scanning electron microscope

Question 17

What three factors affect what is seen under a microscope?

Answer 17

Magnification
Contrast
Resolution

Question 18

When colours are used to increase contrast, showing specific parts of the cell. This is known as….

Answer 18

Staining

Question 19

Steps
Laser concentrates light onto a specimen
Reflection is passed through a detector and each image is a layer of a 3d object.
What is thus defined as… As well what lights can be used along with this.

Answer 19

Confocal technology

Brightfield
Uv
Visible.

Question 20

Magnifies 1500 000x
Scans through the specimen
Sees thin slices/ tiny

Answer 20

Tem

Transmission electron microscope

Question 21

Can magnify up to 300 000x
Sees surface of specimen
Not as clear as similar microscope

Answer 21

Scanning electron microscope

Question 22

Both analyze DNA and gene mapping,
Gfp
3d structure of molecules
Cell communication

Answer 22

Scanning tunnelling microscope

Atomic force microscope

Question 23

Using xrays that scatter to project an image onto a computer screen
Sometimes used to determine DNA

Answer 23

X ray crystallography

Question 24

GFP
Attach to cells to study parts
Aids in alzhelmers study and parkinsons disease research
Only visible under UV light

Answer 24

Green fluorescent protein technology