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Pathology - Kaplan > Chapter 1 - Fundamentals of Pathology > Flashcards

Chapter 1 - Fundamentals of Pathology Flashcards

1
Q

In light microscopic examination of tissue, what’s the gold standard stain?

A

Hematoxylin and eosin (H&E)

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2
Q

Hematoxylin binds…

A

Nucleic acids and calcium salts

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3
Q

Eosin stains the…

A

Majority of proteins (both extracellular and intracellular)

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4
Q

Hematoxylin stains blue to purple:

A

Nuclei Nucleoli Bacteria Calcium

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5
Q

Eosin stains pink to red:

A

Cytoplasm Collagen Fibrin RBCs Thyroid colloid

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6
Q

Prussian blue stains…

A

Iron

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7
Q

Congo red stains…

A

Amyloid

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8
Q

Acid fast (Ziehl-Neelsen, Fite) stains…

A

acid-fast bacilli

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9
Q

Periodic acid-Schiff (PAS) stains…

A

high carbohydrate con- tent molecules

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10
Q

Trichrome stains…

A

cells and connective tissue),

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11
Q

Reticulin stains…

A

collagen type III molecules

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12
Q

Immunohistochemical (antibody) stains: cytokeratin stains…

A

Epithelial cells

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13
Q

Immunohistochemical (antibody) stains: vimentin stains…

A

Cells of mesenchymal origin except the 3 muscle types and many sarcomas

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14
Q

Immunohistochemical (antibody) stains: desmin stains…

A

Smooth, cardiac, and skeletal myosin

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15
Q

Other Immunohistochemical stain:

A

Prostate specific antigen

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16
Q

Molecular techniques include

A

Protein electrophoresis Southern and Western blots Polymerase chain reaction (PCR) Cytogenetic analysis (e.g., karyotyping)

Pathology - Kaplan (4 decks)

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