Chapter 1: Cell Structure Flashcards
How many millimetres in a metre
1000mm
How many micrometers in a metre
10^6 Mm
How many nanometers in a metre
10^9 nm
How many metres in a Mm and nm
Mm- 10^-6 m
nm- 10^-9 m
What is magnification
Number of times greater an image is than an object
What is formula for magnifications
M=I/A
image/actual
What are cell organelles?
Structurally and functionally distinct parts of cells that have their own functions
Nucleus is enclosed by a _ made up of _ also called _
Double membrane
Outer and inner membranes
Nuclear envelope
Nucleus continues to form ER. What do these 2 aid in? (#4)
Ribosome, nucleotide, hormone, ATP production
What are the raw materials for protein synthesis in a non dividing cell
RNA
Ribosomes
What makes up the chromatin network? How does it behave during cell division?
Loosely coiled threads of DNA and proteins called histones
Condenses (wraps tightly around histones and coils further) to form chromosomes containing genes in DNA
Function of nucleolus?
Ribosomal factory with it’s own DNA and RNA
Function of nuclear pore :
Controls material exchange between nucleus and cytoplasm
What is transcription?
When DNA is converted to mRNA in nucleus
(gene switched on, RNA polymerase at promoter, unzipped gene, complementary RNA bases, modifications to remove noncoding regions)
What is translation?
When mRNA is converted to proteins in the cytoplasm/ribosomes
(ribosome reads, tRNA brings amino acids(20) each of 3 bases- codons, and form a chain)
ER has _ membrane
1
Appearance of rough ER + function:
Ribosomes present
Protein synthesis
Appearance of smooth ER + function:
No ribosomes
Lipid, cholesterol, steroid synthesis
A ribosome is a _
Non membrane bound organelle
Where are 70S ribosomes found?
Prokaryotes (bacteria), mitochondria, chloroplasts
Where are 80S Ribosomes found?
Eukaryotes
What are Ribosomes made of
rRNA
Proteins
What is the Svedberg unit and what does it depend on?
Measure of ribosome size
Rate of sedimentation in centrifuge
What are the functions of Golgi complex?
Collection
Modification
Sorting of proteins and lipids
And of course distribution
How is a protein modified? Give 4 ways
- Glycosylation- addition of glucose residue to form glycoprotein
- Methionine amino acid group is removed to convert inactive protein to active one
- Phosphorylation- addition of phosphate group
- Protein folds into tertiary/quarternery protein
How are lipids modified? 2 ways
Glycosylation- giving glycolipid
Phosphorylation- giving phospholipid
Protein/lipid loaded vesicles from ER fuse with Golgi body at _ end and leave at _ end
Cis/forming end
Trans/budding
The secretory pathway has 2 targets. Either _ or _
Other organelles eg lysosome
Cell membrane; vesicles fuse with it and contents are thrown out - exocytosis
Lysosomes have _ and are a site of _
Hydrolytic enzymes
Intracellular digestion
How do lysosomes breakdown 1)old organelles 2)damaged cells
1) organelle is unfolded by membrane into phagocytic vacuole, +enzymes, egestion
2) it breaks and spreads contents across cytoplasm
Sperm heads have _ for _
Acrosome
Destroy path to egg
4 functions of lysosome
Old organelles breakdown
Old cells breakdown
Foreign disease organisms breakdown
Sperm head
pH of lysosome is
Acidic
Outer mitochondrial membrane is _ and consists of aqueous _ called _ which _
Permeable
Protein channels
Porins
Passes small water soluble molecules
pH of intermitochondrial space is
Acidic (H+)
Inner mitochondrial membrane is _ and has folds called _ to increase _ for _
Semi permeable
Cristae
Surface area
Efficiency of respiration/ATP production
The matrix is site of
Krebs cycle/aerobic respiration
Where is Atp synthase found and it’s function?
Cristae
Atp synthesis
Mitochondria have _ ribosomes
70S
Mitochondria are able to replicate because?
They have circular DNA
Function of mitochondria is
Acts as powerhouse for cells/ATP production
Chloroplasts are site of
Light reactions of photosynthesis
State chloroplasts membranes and eachs nature
Envelope made of
Outer- permeable
Inner- semipermeable
Why can chloroplasts self replicate
Have own circular DNA
What is the matrix of a chloroplast? It’s the site of?
Stroma
Dark reactions of photosynthesis
State the main structures in chloroplasts which capture light
Grana are stacks of thylakoids which house chlorophyll pigment and electron carriers
Light reactions produce - and - which provide raw material for - and the end product is -
ATP
NADPH
Dark reaction
Starch
What is the Endosymbiotic Theory?
Eukaryotic cell originated from a symbiotic relationship among prokaryotes
Free living Prokaryotes such as ancestral aerobic/photosynthetic bacteria began living inside eukaryotes and were modified as to be useful to the host cell and make it efficient
Why can mitochondria and chloroplasts now not grow outside host cells?
They lost cell walls and much of DNA
They aren’t likely to adapt too quickly
3 evidences for Endosymbiosis?
Like bacteria;
- chloroplasts and mitochondria divide by binary fission
- own circular DNA
- own 70S ribosomes
What is cell membrane made of
Phospholipids + transport proteins
Function of cell membrane?
Controls substance exchange between cell and it’s environment ie boundary
Nature of cell membrane is
Semipermeable
2 modifications of cell membrane with regard to microvilli
They increase surface area of small intestine for absorption of digested food
Line pct of nephron for maximum reabsorption
1 microtubule made of _ _
13 protofilaments
Protofilaments are made of
Tubulin proteins; α and β, which together form a dimer by polymerisation . This dimer is a helical ring with hollow centre
Where does assembly (polymerisation) and disassembly of microtubules occur?
MTOC
microtubule organizing centre
How are MTOC in animal cells
Centrosomes
How are MTOC in plant cells
Absent
How are MTOC in organisms having cilia and flagella ie beating movements
Centriole OR basal body
3 functions of microtubules?
- acts like a cytoskeleton holds cell in place
- intracellular transport ie movement of organelles, vesicles to organelles
- form spindle fibres for separation of chromosomes during cells division
State the composition of centrosome and Centriole
Centrosome : 2 centrioles perpendicular
Centriole : 9+0 arrangement of 9 microtubule triplets
Location of microtubules
Cytoplasm
What are Paramecium
Unicellular ciliate
Moves and sweeps prey using cilia
What are Euglena
Unicellular flagellate (2 flagellae) Motor to push through water
How do plants produce spindle fibres
MTOC in centrosome region