Chapter 1: Cell Structure Flashcards
What are the basics units to living organisms?
Cells
What piece of equipment is used to view cells?
Microscope
Describe how a light microscope works
Light rays pass through the specimen on a slide and are focused by an objective lens and an eyepiece lens. This produces a magnified image of the specimen on the retina of your eye
Name some alternative ways to view the images formed by a light microscope.
- Screen projection
- Camera Recordings
How does an electron microscope work?
It uses beams of electrons. The electrons are focused onto a screen, or onto photographic film, where they form a magnified image of the specimen.
What are the conditions required for an electron microscope to work well?
The specimen has to be very thin and must be placed in a vacuum, to allow electrons to pass through it
Define Magnification.
Magnification is the size of an image
divided by the size of the actual object
Define Resolution.
Resolution is the size of the smallest
objects that can be distinguished.
Describe Resolution.
It is the ability of the microscope to distinguish two objects as separate from one another. The smaller the objects that can be distinguished, the higher the
resolution.
What is the main determining factor for resolution?
the wavelength of the rays that are being
used to view the specimen.
Why do electron microscopes have a a higher resolution?
The wavelength of a beam of electrons is much
smaller than the wavelength of light.
Name the 3 units used to measure cells.
mm, µm, and nm
how many meters is 1 mm?
1 × 10−3 m
how many meters is 1 µm?
1 × 10−6 m
how many meters is 1 nm?
1 × 10−9 m
What is the formula for magnification?
Actual size
What is the eyepiece graticule?
It is a little scale bar that you can place
in the eyepiece of your light microscope.
How is an eye piece graticule and stage micrometer used to measure the length of a specimen?
- the length of the specimen is found in graticule units.
- Use the stage micrometer to calibrate the graticule, by replacing the specimen. then line the them up.
- Find the ratio between the values of graticule units and stage micrometer units.
- Then, find the real length of the specimen.
What is the stage micrometer?
A stage micrometer is the term typically referring to a slide that comes with a scale on its surface.
What is calibration?
It is the comparison of the grid or scale on the eyepiece graticule with the scale markings of a known dimension on a stage micrometre
What is the cell surface membrane?
is a double layer of lipids and proteins that surrounds a cell and separates the cytoplasm from its surrounding environment
why do cells have many other membranes?
To make different compartments so that different chemical reactions take place with out interfering with one another.
What is the main function of a cell surface membrane?
It controls what enters and leaves the cell.
what is the nuclear envelope?
It is a pair of membranes that surround the nucleus.
What is a chromosome?
A very long molecule of DNA contained inside the nucleus.
What is the role of DNA?
It determines the sequences in which amino acids are linked together in the cytoplasm to form protein molecules.
What does the nucleolus look like?
It is a darker area within the nucleus.
What is the role of the nucleolus?
It is where new ribosomes are made, following the code given by the DNA.
What are ribsomes?
They are small structures made of RNA and protein.
Where are ribosomes found?
They are found free in the cytoplasm and attached to the rough endoplasmic reticulum.
What is the ribosome in the RER and cytoplasm?
80S
what does an 80S ribosome mean?
They are larger than the ones found in mitochondria and chloroplasts.
What is the Rough endoplasmic reticulum?
It is an extensive network of membranes in the cytoplasm. The membranes enclose small spaces called cisternae.