Chapter 1: Cell Structure Flashcards
What is cell theory?
Cell theory states that the basic unit of function and structure of all living organisms is the cell.
What structures do plant and animal cells have in common?
- Cell surface membrane
- Nucleus
- Cytoplasm
- Mitochondria
- Golgi complex
What is a cell?
It is the smallest unit of life.
What is magnification?
Magnification is the number of times greater an image is compared to the actual size of the object.
Describe the two scales present in the light microscope and how they work.
The eyepiece graticule is a transparent disc that commonly has 100 divisions. It is placed in the eyepiece so that it can be viewed at the same time as the specimen to be measured. To calibrate the eyepiece graticule, a transparent scale known as the stage micrometer is placed on the microscope stage and has subdivisions of 0.1 and 0.01 mm. The images of the two scales can then be superimposed.
Give an example of a temporary stain used for staining plant cells
Iodine in potassium iodide solution. It stains starch blue-black and will also colour the nuclei and cell walls a pale yellow.
Give an example of stain used to prepare animal cells.
A dilute solution of methylene blue.
How does a light microscope work?
Once the microscope is switched on, the light bulb comes on. The rays of light travel upwards and illuminate the stage. The light is focused onto the stage using the condenser lens and the amount of light is controlled by the condenser iris diaphragm. The image is picked up by the objective lens which magnifies the image (4, 10, 40, 100) to be seen when viewing it through the eyepiece.
In which two ways can a specimen be prevented from drying out?
A cover slip can be placed over the specimen to protect eh microscope lens and to help prevent the specimen from drying out. A drop of glycerine mixed with the temporary stain added can also prevent drying out.
What are the disadvantages of using electron microscopes?
The electron beam and therefore the fluorescent screen as well as the specimen must be placed in a vacuum. This is because if electrons were to collide with air molecules, they would scatter, making it impossible to produce a sharp image. Furthermore, water boils at room temperature in a vacuum. this means that only deal material can be examined as the specimen would have to be dehydrated before being placed in a vacuum.
What are the two types of microscope?
Light and electron microscope
What is a TEM and how does it work?
In the transmission electron microscope, a beam of electrons is passed through the specimen before being viewed and only those electrons which pass through are viewed enabling thin sections of the specimen to be seen.
What is an SEM and how does it work?
In the scanning electron microscope, the beam of electrons is used to scan the surfaces of structures and only the reflected beam is observed.
What is resolution?
Resolution is the ability to distinguish between two objects very close together. The higher the resolution, the greater the detail that can be seen.
What is the disadvantage of the SEM compared to the TEM?
The SEM cannot achieve the same resolution as the TEM. With an SEM, a resolution between 3nm and 20 nm is possible.
What is the electromagnetic spectrum?
The whole range of different wavelengths is known as the electromagnetic spectrum.
What is the general rule regarding wavelength and resolution?
The general rule states that the limit of resolution is one half the wavelength of the radiation used to view the specimen.
What is the wavelength of radiation using a light microscope?
400-700nm
When calculating total magnification, what is multiplied together?
eyepiece lens magnification and the objective lens magnification
Why are electrons suitable for microscopy?
Free electrons behave like electromagnetic radiation and have a very short wavelength due to having a greater amount of energy. The greater the energy, the shorter the wavelength. Also, due to the negative charge of electrons, they can be focused easily using an electromagnet.
With which two factors does the wavelength change?
The higher the frequency, the shorter the wavelength. Wavelength changes with energy: The greater the energy, the shorter the wavelength.
Define ultrastructure.
Ultrastructure is the fine detailed structure of a specimen as revealed by the electron microscope.
Describe the images obtained using an electron and light microscope.
Coloured photomicrograph.
Black and white electron micrograph.
Describe the structure of a nucleus.
The nucleus is the largest cell organelle present in a cell and is surrounded by a double membrane made up of a double phospholipid bilayer. The nuclear envelope contains many small gaps and the outer membrane is continuous with the rough endoplasmic reticulum. Within the nucleus, chromosomes can be seen in a loosely coiled state with 1-5 nucleoli.
Describe the structure of the nuclear envelope.
The nuclear envelope is made up of two membranes. Each membrane is made up of a phospholipid bilayer, hence making the nuclear envelope a double phospholipid bilayer. The outer membrane of the nuclear envelope is continuous with the endoplasmic reticulum. The nuclear envelope contains many gaps within its walls.
How are the images viewed in the light and electron microscope?
Light: eyepiece
electron: fluorescent screen
What are nuclear pores and its function?
Nuclear pores are gaps in the nuclear envelope that allow and control the exchange between the nucleus and cytoplasm.
What are some differences between animal and plant cells? 3:2
Animal: Centrioles, microvilli
Plants: Cell wall, Vacuoles, chloroplasts
What are some features that animal and plant cells have in common? (5)
Cell surface membrane, nucleus, mitochondria, golgi bodies, cytoplasm.
What are the lens used in the light and electron microscope?
Light: glass
Electron: Electromagnet
What do the stains used in electron microscopy contain?
heavy metal atoms
What is an organelle?
An organelle is a functionally and structurally distinct part of a cell, usually membrane bound.
What is compartmentalisation?
Organelles are often surrounded by membrane(s) which keep its processes separate from the surrounding cytoplasm. This is known as compartmentalisation.
What is the division of labour?
Since each type of organelle has its own specific function, the cell is said to show division of labour, which is the sharing of work between different specialised organelles.
What is the function of the cell surface membrane?
It is a partially permeable barrier that controls the exchange of molecules and ions between the cell and its environment.
What is the maximum resolution possible with a light microscope?
200nm
What is the maximum resolution possible with an electron microscope?
0.1-0.5nm
What is the structure of the cell membrane?
The cell surface membrane is extremely thin and has a trilaminar appearance consisting of two dark lines on either side of a pale narrow interior. This appearance is due to the phospholipid bilayer with hydrophilic phosphate heads facing outwards and hydrophobic tails facing inwards.
What is the usual measurement of a cell surface membrane?
7nm
What is the usual measurement of a nucleus?
10 um
What is the wavelength of radiation using an electron microscope?
+-0.005nm
Describe the structure of the rough endoplasmic reticulum.
The outer membrane of the nuclear envelope is continuous with the rough endoplasmic reticulum. The RER is so called as it is covered with many tiny black dots known as ribosomes giving it a rough appearance.
Describe the structure of the smooth endoplasmic reticulum.
It is a meshwork of tubular membrane vesicles with fluid filled sacs that have no ribosomes on its surface.
Explain how compartmentalisation applies in the endoplasmic reticulum.
Processes can take place within the membrane-bound flattened sacs, separated from the surrounding cytoplasm.
Name the substances entering the nucleus through the nuclear pores. (4)
Proteins for ribosome synthesis, nucleotides, ATP, some hormones such as thyroid hormone T3.
Name the substances leaving the nucleus through the nuclear pores. (2)
mRNA and ribosomes for protein synthesis.
Specify which type of ribosome is found where.
80S ribosome: on the rough endoplasmic reticulum and free in the cytoplasm
70S ribosome: in chloroplasts and in mitochondria
What are ribosomes made of?
RNA and protein
What are the types of endoplasmic reticulum?
Smooth and rough
What divides first when a cell is about to divide?
The nucleus divides first so that each new cell has its own nucleus.
What do chromosomes contain?
Chromosomes contain the hereditary material DNA that is organised into functional units known as genes which controls the activities of the cell and inheritance.
What happens to the proteins produced by the ribosomes on the RER?
They enter the sacs and move through them. The proteins are often modified on their journey.
What is a nucleolus made up of?
It is made up of loops of DNA from several chromosomes and RNA.
What is chromatin?
Within the nucleus, chromosomes are in a loosely coiled state known as chromatin.
What is the endoplasmic reticulum?
The ER is an extensive system of membrane-bound flattened sacs running through the cytoplasm.