Chap5

Question 1

Issues to consider in choosing a particular samples:

Answer 1

a. Technical requirements
b. time efficiency
c. cost effectiveness of the chosen method

Question 2

is one of the most commonly used method for isolation and enrichment of human mononuclear cells, particularly from peripheral blood and other biological fluids, e.g.,
umbilical cord
blood and bone marrow

Answer 2

Ficoll density gradient centrifugation

Question 3

Whole blood can be routinely collected for plasma and peripheral blood mononuclear cells isolation using centrifugation and a density gradient medium such as

Answer 3

Ficoll-Hypaque