Chap 4: Enzymes Flashcards
What is a catalyst?
A substance that can speed up a chemical reaction without being chemically changed itself.
What are enzymes?
Enzymes are proteins that function as biological catalysts, speeding up chemical reactions and remaining unchanged at the end of the reaction.
How do enzymes lower activation energy?
Enzymes provide an alternate pathway for the reaction with lower activation energy, making it easier for the reaction to occur.
What is the role of enzymes in digestion?
Enzymes involved in digestion are called digestive enzymes, and they help break down large molecules into simpler substances that can be absorbed.
What is the lock-and-key hypothesis?
According to the lock-and-key hypothesis, enzymes and substrates have specific complementary shapes, like a lock and key, allowing them to bind together.
What is an enzyme-substrate complex?
It is a temporary molecule formed when the substrate binds to the active site of the enzyme, leading to a chemical reaction.
What are the characteristics of enzymes?
Enzymes speed up reactions, are specific in their action, required in small amounts, and remain unchanged at the end of the reaction.
How are enzymes affected by temperature?
Enzymes are less active at low temperatures, have an optimum temperature where they are most active, and denature at high temperatures.
What is denaturation?
Denaturation is the alteration of the three-dimensional structure of an enzyme or protein, usually caused by heat or chemicals, resulting in loss of function.
How does pH affect enzymes?
Different enzymes work best at specific pH levels, and deviations from their optimal pH can affect their activity.
What is the role of amylase?
Amylase is an enzyme that breaks down starch into smaller sugars like maltose.
What is the function of protease?
Protease is an enzyme that breaks down proteins into smaller peptides or amino acids.
What is the optimum temperature for human enzymes?
Human enzymes are most active around 40-45 degrees Celsius.
How does temperature affect enzyme activity?
Increasing temperature increases enzyme activity until the optimum temperature is reached, after which enzyme activity decreases due to denaturation.
What is the importance of enzyme specificity?
Enzyme specificity ensures that enzymes only bind to and catalyze specific substrates, preventing unwanted reactions.
What is the significance of enzymes being reusable?
Enzymes can be used repeatedly in reactions, making them highly efficient and allowing a small amount of enzyme to catalyze a large amount of substrate.
What is the role of lipase in digestion?
Lipase is an enzyme that breaks down fats into fatty acids and glycerol.
How are enzymes affected by denaturation?
Denatured enzymes lose their three-dimensional structure, including their active site, and can no longer function as catalysts.
Why do enzymes have an optimum pH?
Enzymes have an optimal pH at which their active site is in the best conformation for catalysis.
What happens to enzyme activity when pH deviates from the optimal range?
Enzyme activity decreases when pH deviates from the optimal range, as the conformation of the active site is disrupted.
What are the different enzymes involved in digestion?
The enzymes involved in digestion include amylase, protease, lipase, and others specific to breaking down carbohydrates, proteins, and fats.
What is the role of amylase in digestion?
Amylase breaks down starch into smaller sugar molecules, such as maltose, to facilitate their digestion and absorption.
What is the function of protease in digestion?
Protease enzymes break down proteins into smaller peptides or individual amino acids for digestion and absorption.
What does lipase do in digestion?
Lipase is responsible for breaking down fats or lipids into fatty acids and glycerol, enabling their digestion and absorption.