Ch 5: Diagnostic Microbiology

Question 1

What does successful identification of microbe depend on?

Answer 1

Using aseptic technique, correctly obtaining and handling the specimen, quickly transporting the specimen to the lab

Question 2

Name three methods used to identify microorganisms

Answer 2

Classic microbiology, molecular microbiology (genetic), immunological analysis

Question 3

What are the five steps in the classic microbiological route to obtain a pure culture?

Answer 3

Inoculation, Incubation, Isolation, Inspection, Identification

Question 4

What is Inoculation?

Answer 4

Introduce bacteria into a growth medium using “aseptic technique” to prevent contamination

Question 5

What is Incubation?

Answer 5

Allow organisms to grow under the optimal conditions (temp, with or without oxygen…)

Question 6

_____ is an enzyme that neutralize toxic forms of oxygen metabolites (H2O2)

Answer 6

Catalase

Question 7

ToF: Anaerobic bacteria usually have the catalase enzyme

Answer 7

F

Question 8

What does catalase break down?

Answer 8

Breakdown hydrogen peroxide (H2O2) into oxygen and water

Question 9

What does the oxidase test confirm?

Answer 9

If the microbe is aerobic or anaerobic

Question 10

How does an oxidase test work?

Answer 10

It identifies bacteria that produce cytochrome c oxidase, an enzyme of the bacterial electron transport chain

Question 11

ToF: In an oxidase test, an anaerobic microbe will result in a colour change

Answer 11

F: Colour change occurs when cytochrome c oxidase present in aerobic bacteria oxidizes the agent to purple colour end product,