ch 5 Flashcards
standardizing
the process of determining the relationship between the signal and the amount of analyte in a sample or a standard
primary standard
very pure substance used to quantify an unknown
secondary standard
can be used in conjunction with a primary standard
reagent
implies a purity of a solvent or ingredient
serial dilutions
use standard solution….
standard solution
a solution in which the concentration is very well known
Why do analytical chemists use serial dilutions?
they are better for obtaining a solution with a super high volume of dilutant with a super small (0.0000g) amount of stock being diluted
aliquot
a well defined portion of stock solution
How does one determine the amount of stock to use to make a certain concentration of aliquot?
M1V1=M2V2
M1: molarity of stock
V1:(x)
external standard
standard material is not mixed with the sample … standards are separate
Single Point Calibration (1)
- type of standardization
- not realistic
- ppb/absorbance
Multiple External Standards (2)
-include at least 3 standards
-concentration vs response plot
…..extrapolation
……interpolation
…………………..linear regression
extrapolation
if a sample is found not to be on the/ out side of the curve… try to get concetration from point ………..you have to dilute if outside of range
interpolation
sample within range….?
linear regression
the goal of this type of analysis is to determine the best estimates for b_0 and b_1 in
y=Bo+Blx