Ch 12 - Cell Division Flashcards
Multicellular eukaryotes depend on cell division for:
- development from fertilized cell
- growth
- tissue repair
System by which prokaryotes, mitochondria, and chloroplasts reproduce
Binary fission
3 steps of binary fission
1) bacterial DNA (chromosome) replicated
2) cell elongates, pulling 2 copies of DNA apart
3) plasma membrane pinches inward, dividing the cell into 2
When fully stretched, E. coli genomic DNA is _____ times as long as the cell
500
Under optimal conditions, e. Coli cells divide every ___ minutes
20
Genomic DNA of E. coli must be copied every ____ minutes, meaning ______ base pairs copied every second
20; 3866
Genomic DNA of E. coli is attached to ______, near ___________
Plasma membrane; origin of replication
Eukaryotic chromosomes
Contain more DNA than E. coli
DNA organized in separate chromosomes
Chromatin
Complex of DNA and proteins
Histones
Major proteins in chromatin
Chromosome condensation
When chromosomes undergo several levels of coiling and become shorter, thicker and more compact
Reduces the length of chromatin by 10,000
Cohesins
Proteins that attach chromatids
Centromere
Constriction where sister chromatids are most closely attached
Once sister chromatids are separated, the chromatids are called _______
Chromosomes
3 steps of the cell cycle
1) DNA replication
2) mitosis
3) cytokinesis
2 phases of the cell cycle
Mitotic phase (mitosis and cytokinesis) Interphase (G1, S, G2)
Stages of mitosis
Prophase Prometaphase Metaphase Anaphase Telophase
Prophase
DNA in nucleus condenses
Becomes tightly packed until each pair of chromatids forms a distinct chromosome
Spindle fibers begin to form
2 nucleoli disappear
Prometaphase
Chromosomes continue to condense
Nuclear membrane fragments
Microtubules of the spindle begin to attach to the sister chromatids
Metaphase
Chromosomes align on equatorial plane of cell
Alignment occurs when spindle microtubules push and pull on the chromosomes
Stage where chromosomes are most condensed
Anaphase
Shortest phase of mitosis 2 sister chromatids are pulled apart -cohesion proteins are broken down Spindle microtubules shorten as they break down at the 2 poles Drags chromatids towards the poles Cell elongates
Telophase
Nuclear envelopes form around the separated chromosomes Chromosomes become less condensed Nucleoli reappear Spindle fibers break down Mitosis complete
Mitotic spindle
Forms in the cytosol during prophase
Consists of fibers made of microtubules and proteins
Assembly starts at the centromere (microtubule-organizing center)
Spindle microtubules grow out from centromeres toward equatorial plane
Aster
Radial array of short microtubules extending from each centromere