Ch. 10 Oxidative Phosphorylation

Question 1

What are the 4 steps of the electron transport chain (ETC)?

Answer 1

1. Electrons transferred
2. Protons pumped
3. Oxygen reduced
4. ATP generated

Question 2

Which complexes does NADH go through?

Answer 2

1, 3, and 4

Question 3

Which complexes does FADH2 go through?

Answer 3

2, 3, and 4

Question 4

How many protons are pumped through complex I with a molecule of NADH?

Answer 4

4

Question 5

How many protons are pumped through complex III when processing a molecule of NADH? FADH2?

Answer 5

Both pump 4

Question 6

How many protons are pumped through complex IV when processing a molecule of NADH? FADH2?

Answer 6

Both pump 2

Question 7

How many protons does a molecule of NADH yield?

Answer 7

10

Question 8

How many protons does a molecule of FADH2 yield?

Answer 8

6

Question 9

What does Complex V use a proton gradient for?

Answer 9

To form ATP

Question 10

How many ATP synthase rotations does a molecule of NADH allow for?

Answer 10

1

Question 11

How many ATP synthase rotations will a molecule of FADH2 allow for?

Answer 11

2/3

Question 12

How many ATP will a full ATP synthase rotation yield?

Answer 12

3

Question 13

How many ATP will a 2/3 ATP synthase rotation yield?

Answer 13

2

Question 14

What are the 3 conformations/shapes of the ATP synthase binding mechanism?

Answer 14

1. Open
2. Loose
3. Tight

Question 15

Describe the open phase of the ATP synthase binding mechanism.

Answer 15

ADP and Pi bind to beta subunit

Question 16

Describe the loose phase of the ATP synthase binding mechanism.

Answer 16

Gamma subunit rotates 1/3 of the way. ADP and Pi are locked in but not close yet.

Question 17

Describe the tight phase of the ATP synthase binding mechanism.

Answer 17

The gamma subunit rotates another 1/3. ADP and Pi form ATP.

Question 18

Describe what happens after the tight phase of the ATP synthase binding mechanism.

Answer 18

Gamma subunit completes full rotation to release 3 ATP.