CGH Flashcards
Alterations of the DNA of a genome that results in the cell having an abnormal number of copies of one or more sections of the DNA
Copy-number variations (CNVs)
Amplifications and deletions can contribute to ___
tumorigenesis
___ is the most common change seen in malignancies
Amplification
T/F: Detection and mapping provides an approach to associate an aberration with a disease phenotype and localising critical genes-Biomarkers and can be done by FISH and CGH
T
T/F: CGH is to body fluids, FISH is to solid tumors
F (CGH = solid tumors; FISH = body fluids)
T/F: CGH is a pre-FISH technique
T
A molecular-cytogenetic method for the analysis of copy number changes (gains/losses) in the DNA content of a given subject’s DNA and often in tumor cells
CGH or CMA
CGH was first described in ___ by ___ et al.
1993, Kallioniemi
T/F: CGH cannot detect balanced changes such as substitutions, translocations, and inversions
T
In CGH method, DNA are unwrapped to single-stranded structures via ___ treatment
Alkaline
A special FISH technique that is applied for detecting all genomic imbalances
CGH
The basics of CGH is comparison of total genomic DNA of the given ___ ___ with total genomic DNA of ___ ___
sample DNA
normal cells
A fluorescent microscope equipped with a ___ ___ ___ camera and an image analysis system are used for evaluation
Charge coupled device (CCD)
Used to determine copy number alterations of genome in cancer and those cells whose karyotype is hard or impossible to prepare or analyze
CGH
T/F: CGH will detect only unbalanced chromosomal changes
T
T/F: Structural chromosome aberrations such as balanced reciprocal translocations or inversions can not be detected by CGH, as they do not change the copy number
T
CGH is based on ___-___ of two differentially labeled genomic DNAs to human metaphase chromosome spreads
onco-hybridization
The metaphase spreads used in CGH are usually prepared from ___ ___ ___ of a healthy donor
peripheral blood lymphocytes
The signal intensity ratios of the two labels along the chromosomes then reflect DNA copy number changes in the test genome relative to the reference genome, wherein the resolution is limited to about __ to __ Mb
3-10
Color of FITC
Green
Color of TRITC
Red
The genomes are combined with an excess of ___-_ DNA and hybridised to metaphase chrmosomes
cot 1
___ hybridization due to repetitive DNA sequences is a common problem in assays
background
___-_ DNA blocking reagent blocks repetitive DNA sequences and prevents nonspecific hybridization
Cot-1
