CFP II Block 2

Question 1

Basal Transcription Machinery

Answer 1

composed of RNA polymerase II and some general transcription factors

Question 2

Cis- Regulatory Sequence

Answer 2

Regions of DNA that regulate the expression of genes when bound by transcription factors. Can be close to or far away, promotor, enhancer, silencer.

Question 3

Co-Activator

Answer 3

This protein helps transcription factors to bind to the basal machinery. It is often found between activators and the basal machinery

Question 4

Activator

Answer 4

Nuclear proteins which bind to enhancers and the basal machinery to increase transcription levels

Question 5

Repressor

Answer 5

Nuclear proteins that bind to silencers and the basal machinery to decrease transcription levels

Question 6

Promotor

Answer 6

This is the sequence of DNA where RNA polymerase II and general transcription factors bind to initiate transcription

Question 7

Enhancer

Answer 7

Cis-regulatory sequence that increases transcription levels when activators bind to it

Question 8

Silencer

Answer 8

Cis-regulatory sequence that reduces transcription levels when repressor bind to it

Question 9

General Transcription Factors

Answer 9

Transcription factors are always found associated with RNA polymerase II when gene needs to be transcribed and is part of the basal machinery

Question 10

Restriction enzymes (endonuclease)

Answer 10

an enzyme that cleaves (cuts) DNA at or near specific recognition nucleotide
-Type II most common

Question 11

Expression Vectors

Answer 11

a plasmid or virus designed for protein expression in cells

Question 12

ligase

Answer 12

joins

Question 13

Terminal deoxynucleotidyl transferase

Answer 13

add complimentary sticky ends