Certification 2 Flashcards
Circulatory System
Delivers o2, nutrients, hormones, and enzymes to the cells; transports cellular waste to organs where they can be expelled. Blood is vehicle; blood vessels, tubes, and heart work as pump.
Pulmonary circulation
carries deoxygenated blood from right ventricle to lungs to left atrium.
Systemic circulation
Carries oxygenated blood from left ventricle throughout the body
Valves
Tricuspid, bicuspid(mitral), aortic, and pulmonic
Tricuspid
Atrioventricular valve that is between right atrium and right ventricle
Bicuspid(mitral)
Atrioventricular valve between left atrium and right ventricle
Aortic valve
Semi lunar valve between left ventricle and aorta
Pulmonic valve
Semi lunar valve between right ventricle and pulmonary artery
Endocardium
Inner layer lining of heart
Myocardium
Muscular middle layer
Epicardium
Fibrous outer layer; coronary arteries that supply blood to heart are found here
How many liters of blood does average adult have?
5-6 liters
Layers of blood vessels
Tunica adventitia
Tunica media
Tunica intima
Tunica adventitia
Outer connective tissue layer
Tunica media
Middle smooth muscle
Tunica intima
Inner endothelial layer
Capillaries
Composed of a layer of endothelial cells and are a mix of arterial blood and venous blood
Order of draw for capillaries
Lavender
Tubes with other additives
Tubes without additives
Erythrocyte
Start as immature reticulocytes; in 1-2 days mature; there are 4.2-6.2 million RBCs per microliter. Life span is 120 days; contain hemoglobin/oxygen carrying protein.
Leukocytes
Body’s protection from infection/illness; average adult has 5,000 10,000 wbc
Neutrophils
Comprise 40-60% of WBCs; phagocytic and engulf and digest bacteria
Lymphocytes
20-40% of WBCs; immunity
Monocytes
3-8% of WBCs; largest WBCs; they’re monocytes in circulating blood, but when absorbed in tissues transform to macrophages(extremely powerful phagocytes); increase with intracellular infection
Eosinophils
1-3% of WBCs; active against antibody-labeled foreign molecules. Increase in allergies, skin infection, and parasitic infection
Basophils
0-1% of WBCs; carry histamine releases during allergic reactions
Thrombocytes(platelet)
Packets of cytoplasm that are formed in bone marrow from megakaryocytes. Average amount in an adult is 140-440,000 microliters of blood; lifespan is 9-12 days
Hemostasis
Process where the blood vessels are repaired after injury.
Vascular phase hemostasis
Injury causes vessel to constrict slowing blood flow
Platelet phase hemostasis
Injury to endothelial lining causes platelets to adhere to it
Coagulation phase hemostasis
Involves various coagulation factor, causing temporary plug to convert to fibrin clot.
Fibrinolysis hemostasis
Breakdown and removal of clot
Chux
Impermeable pad that protects patient clothing and bedding.
Two hour post prandial test
Used to evaluate diabetes mellitus; fasting glucose is compared with glucose levels 2 hours after eating
OGTT
Oral glucose tolerance test-diagnose diabetes mellitus. Evaluate patients with frequent low blood sugar
3 hour OGTT
Test hyperglycemia and diagnose diabetes
5 hour OGTT
evaluate hypoglycemia and disorder of carbohydrate metabolism/ scheduled to begin 7-9 am
Cold agglutinins:
Antibodies are produced in response to Mycoplasma pneumonia infection (atypical). Blood is collected in red top tubes and pre-warmed in an incubator for 30 min. at 37c
Light sensitive specimens
Bilirubin, beta-carotene, vitamins A and B6 and porphyrins
Chilled specimen
Arterial blood gas, ammonia, lactic acid, pyruvate, ATCH, gastrointestinal, and parathyroid hormone.
Order of draw
Blood cultures Sodium citrate blue tops Serum tubes w/ or w/out clot activator or gel tops Heparin tubes-green EDTA lavender Oxalate fluoride gray tops
EDTA
Inhibits coagulation by binding to calcium in specimen
Lavender
Invert 8 times; common tests are CBC, hemoglobin and hematocrit determination, ESR, sickle cell
Sodium citrate
Prevents coagulation by binding to calcium. Preserves coagulation
Light blue
Invert 3-4 times. Pt tests, Coumadin therapy, APTT, PTT, heparin therapy, fibrinogen degradation, thrombin time, factor assays, bleeding time.
Heparin
Combined with sodium, lithium, or ammonium ions. Inhibits thrombin and is not used for hematology because heparin interferes with Wright’s Stained blood smear
Green
Invert 8 times. Tests chemistry, performed on plasma such as ammonia carboxyhemoglobin, and STAT electrolytes
Sodium fluoride
Antiglycolitic agent preserves glucose for 3 days.lithium iodacetate preserves glucose for 24 hours. Potassium oxalate prevents clotting by binding to calcium
Gray top
Invert 8 times. Fasting blood type, GTT, blood alcohol levels, lactic acid.
Red/gray top, gold
SST. Clot activator; glass particles, silica and celites hasten clot formation. Thixotropic gel is serum separator. Invert 5 times
Red top
Plain vacuum tube- no additive/coagulant. Tests are serum chem, serology, and blood bank.
Yellow top
SPS. Cultured for presence of microorganisms. SPS aids in recovery of microorganisms and inhibits actions of complement. Performs phagocytosis on certain antibiotics. Invert 8 times
Electrophoresis
Analyzes chemical components of blood such as hemoglobin, serum, urine, and cerebrospinal fluid. Based in differences in Electrical charges.
Immunochemistry
Uses techniques such as radio immunoassay and enzyme immunoassay detect and measure substances such as hormones, enzymes, and drugs
Two major categories of health care organizations
Hospital or ambulatory
Phlebotomist are often expected to perform
Clinical, technical, and clerical duties
Certification provides phlebotomist with which career advantages
Increased job opportunities, advancement opportunities, and portability.