Central Dogma (Ms. Tan)

Question 1

Main laboratory ware used in dilution plate counts

Question 2

Microorganisms produced per unit substrate utilized

Question 3

CFU

Question 4

Measure particle size distribution of cells in a culture.

Question 5

A measure of the amount of light that passes through a turbid sample.

Question 6

Typically used as seed in batch culture.

Question 7

Oxygen solubility in water at 25 deg C and 1 Atm

Question 8

Typical cause of limited oxygen transfer rate

Question 9

Affect the transport of certain nutrients in and out of the cell

Question 10

Dissolved gas toxic to some organisms at high cone

Question 11

Catabolism of cellular reserves for continued maintenance and energy.

Question 12

Cell growth equation.

Question 13

Cell produce secondary metabolites

Question 14

Adaptation to new environment

Question 15

Cell physiology changes to favor survival over growth

Question 16

cryptic growth occurs

Question 17

extended by low temperature

Question 18

Commonly molded as a 1st order process with respect to biomass

Question 19

Growth balanced

Question 20

Some portion of cells remain viable for a long time, but are altered

Question 21

No dependence on substrate concentration

Question 22

Caused by either build-up of toxic products or depletion of substrate

Question 23

Primary metabolites produced

Question 24

No cell division occurs

Question 25

During Mutations: If the amino acid is the same as before the mutation _______.

Question 26

If the amino acid is different, but not in the _____ of the active site there _______.

Question 27

If the mutation is in the active site there may be ______ consequences.

Question 28

If the mutation changes the amino acid to a ______, the resulting ____ will be _____ and not ____.

Question 29

During natural gene transfer/ _____, once the DNA is inside the ____ it can remain _____ from the chromosome in sell _____ , ______, or integrate into the chromosome.

Question 30

To integrate, the DNA must be ______ to the chromosomal DNA of the _______

Question 31

Explain the transfer cloning of the insulin gene.

Question 32

Explain the process of Nucleic Acid Hybridization

Question 33

Explain the process and principle of Gel Electrophoresis