Cells and Tissues

Question 1

Most common fixative?

Answer 1

Formalin.

Question 2

How does formalin work?

Answer 2

Covalent bonding between proteins.

Question 3

Pros and cons of formalin?

Answer 3

good penetration, but destroys cytoplasmic things.

Question 4

Why is glutaraldehyde good? What for?

Answer 4

Electron microscopy, preserves the cytoplasmic.

Question 5

Haemotoxylin stains what? and is acidic or basic?

Answer 5

Stains acidic structures pruple, is basic itself.

Question 6

Eosin stains what and is what itself?

Answer 6

Stains basic pink, is acidic itself.

Question 7

masons trichome?

Answer 7

HAM - Haemotoxylin, acid fushin and methyl blue

Question 8

Mucin stain?

Answer 8

Periodic acid chiff, but as diastase to get rid of glycogen

Question 9

perles prussian stain use?

Answer 9

for iron, and asbestos in lung tissues

Question 10

What does epithelial basement membrane dystrophy cause?

Answer 10

Corneal erosions, due to the hemidesmone problems

Question 11

ichtyosis caused by what?

Answer 11

Hyperkeratinisation

Question 12

kartageners syndrome due to?

Answer 12

Situs inversus, cillia problems

Question 13

Extracellular matrix made up of?

Answer 13

Ground substance

Question 14

What do fibroblasts make?

Answer 14

make collagen, elastin, glycosaminoglycans

Question 15

What is marfans due to?

Answer 15

Defect in Fibrillin

Question 16

What makes bone?

Answer 16

Osteoblasts B for build

Question 17

What makes ground substance?

Answer 17

Chondroblasts