Cells Flashcards

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1
Q

Cell-surface plasma membrane

Structure and function

A

The membrane found on the surface of animal cells and just inside the cell wall of plant cells. Made mainly of proteins and lipids.
The cell membrane regulates the passage of substances into and out of the cell. Also has receptor molecules which allow it to respond to chemicals like hormones.

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2
Q

Nucleus

Structure and function

A

A large organelle surrounded by a nuclear envelope double membrane that contains many pores. The nucleus contains chromosomes and one or more nucleoli.
The nucleus controls the cells activities. DNA contains instructions to make proteins. The pores allow substances like RNA to move between the nucleus and cytoplasm. The nucleolus makes ribosomes.

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3
Q

Mitochondrion

Structure and function

A

Generally oval shaped with a double membrane. The inner membrane is folded into cristae. Within this is the matrix, which contains enzymes involved in respiration.
Mitochondria are the site of aerobic respiration, where ATP is produced. They’re found in large numbers in cells that are very active and require a lot of energy, such as muscle cells and liver cells.

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4
Q

Chloroplasts

Structure and function

A

Chloroplasts are a small, flattened structure found in plant and algal cells. They are surrounded by a double membrane, as well as having membranes within the organelle called thylakoids. These thylakoids are stacked up to form grana. Grana are linked together by lamellae- thin, flat pieces of thylakoid membrane.
Chloroplasts are the site of photosynthesis. The light dependent stage of photosynthesis occurs in the grana and the light independent stage occurs in the stroma.

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5
Q

Golgi apparatus

Structure and function

A

A group of fluid-filled, membrane-bound, flattened sacs. Vesicles are often seen at the edges of the secs.
The Golgi apparatus processes and packages new lipids and proteins. It also makes lysosomes.

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6
Q

Golgi vesicles

Structure and function

A

Small fluid-filled sacs in the cytoplasm, surrounded by a membrane and produced by the Golgi apparatus.
Stores lipids and proteins made by the Golgi apparatus and transports them out of the cel via the cell-surface membrane.

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7
Q

Lysosome

Structure and function

A

A round organelle surrounded by a membrane, with no clear internal structure. A type of Golgi vesicle.
Contains digestive enzymes called lysozymes, that are kept separate from the cytoplasm by the surrounding membrane. Lysozymes can be used to digest invading cells or break down worn out components of the cell.

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8
Q

Ribosome

Structure and function

A

A very small organelle that either floats free in the cytoplasm or is attached to the rough Endoplasmic Reticulum. It’s made up of proteins and RNA and is not surrounded by a membrane.
The site of protein synthesis

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9
Q

Rough Endoplasmic Reticulum

Structure and function

A

A system of membranes enclosing a fluid-filled space. The surface is covered with ribosomes.
Folds and processes proteins that have been made at the ribosomes

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10
Q

Smooth Endoplasmic Reticulum

Structure and function

A

A system of membranes enclosing a fluid-filled space, but with no ribosomes on its surface.
Synthesises and processes lipids.

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11
Q

Cell wall

Structure and function

A

A rigid structure that surrounds cells in plants, algae and fungi. In plants and algae, it’s made mainly of cellulose, while in fungi it’s made of chitin.

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12
Q

Cell vacuole

Structure and function

A

A membrane-bound organelle found in the cytoplasm of plant cells. It contains cell sap- a weak solution of salts and sugar. The surrounding membrane is called the tonoplast.
Helps to maintain pressure inside the cell and keep the cell rigid. This stops plants wilting. Also involved in the isolation of unwanted chemicals inside the cell

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13
Q

Give 3 specialisations of epithelial cells in the small intestine to absorb food efficiently

A
  • They have microvilli facing the small intestine, which are tiny, finger-like projections that greatly increase the surface area for diffusion and active transport.
  • The epithelial cells themselves are situated at the surface of villi, which are finger-like projections from the walls of the small intestines that increase the surface area.
  • The epithelial cells have lots of mitochondria to provide energy for the transport of digested food molecules into the cell.
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14
Q

What is the difference between the cytoplasm of eukaryotic cells and prokaryotic cells?

A

The cytoplasm of prokaryotic cells has no membrane-bound organelles. It has ribosomes, but they’re smaller than those in eukaryotes.

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15
Q

What is a shared characteristic between prokaryotes and eukaryotes?

A

They have a plasma membrane mainly made of proteins and lipids, which controls the movement of substances into and out of the cell.

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16
Q

What is a flagellum?

A

A long, hair-like structure that protrudes from some prokaryotes and rotates to make the prokaryote move, like the tail of a sperm cell. Some prokaryotes don’t have flagella and some have multiple.

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17
Q

Where is the DNA in a prokaryotic cell?

A

Prokaryotes do not have a nucleus. Instead, the DNA floats free in the cytoplasm. It is circular DNA, present as one long coiled up strand. It is not attached to histone proteins.

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18
Q

What is different about the cell wall of prokaryotic cells?

A

The cell wall has the same function (supporting the cell and maintaining its shape) but it is made of a polymer called murein. Murein is a glycoprotein (protein with a carbohydrate attached)

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19
Q

What are plasmids?

A

Plasmids are small loops of DNA separate from the main circular DNA molecule. They contain genes for things like antibiotic resistance, and can be passed between prokaryotes. Plasmids are not always present in prokaryotic cells, but some have several.

20
Q

Some prokaryotes also have a capsule made up of secreted slime. What is its purpose?

A

It helps to protect bacteria from attack by cells of the immune system

21
Q

What are viruses?

A

Viruses are acellular, meaning they aren’t cells. They are in fact just nucleic acids surrounded by protein, and they aren’t even alive.

22
Q

Name 3 properties of viruses that make them different to bacteria.

A
  • Viruses do not replicate by division, they invade and reproduce inside the cells of other organisms (host cells).
  • Viruses are even smaller than bacteria. For example, HIV is about 0.1 micrometers across.
  • Viruses have no plasma membrane, no cytoplasm and no ribosomes.
23
Q

How is genetic material stored in viruses?

A

Viruses contain a core of genetic material, which can be DNA or RNA.

24
Q

What is the name of the protein coat around the virus core?

A

The capsid

25
Q

What are attachment proteins?

A

These are proteins that protrude from the edge of the capsid and enable the virus to cling onto suitable host cells.

26
Q

Prokaryotic cells replicate by binary fission. Describe this process.

A

The circular DNA and plasmids in the prokaryotic cell replicate. The main DNA loop is only replicated once, but the plasmids can be replicated many times.
The cell then gets bigger and the DNA loops more to opposite poles of the cell.
The cytoplasm then begins to divide and new cell walls begin to form.
The cytoplasm divides completely and two daughter cells are produced. Each daughter cell has one copy of the circular DNA, but can have a variable number of copies of the plasmids.

27
Q

Magnification=

A

Size of object

28
Q

What is resolution?

A

How detailed the image is. More specifically, it is how well a microscope distinguishes between two points that are close together.

29
Q
Optical microscopes
How is an image formed?
What is the resolution?
What can't be seen?
What is the maximum useful magnification?
A

These microscopes use light to form an image.
They have a max resolution of about 0.2 micrometers.
You cannot see organelles smaller than 0.2 um, such as ribosomes, Endoplasmic Reticulum or lysosomes. Mitochondria might not be visible in perfect detail.
The max useful magnification is about x1500.

30
Q

Electron microscopes
How is an image formed?
What is the resolution?

What is the maximum useful magnification?

A

They use electrons to form an image.
They have a higher resolution than optical microscopes, with a maximum of about 0.0002 micrometers, 1000x as high.
The maximum useful magnification is therefore x1500000.

31
Q

Transmission electron microscopes
How do they work?
What is their strength and their weakness?

A

TEMs use electromagnets to focus a beam of electrons, which is transmitted through a specimen. Denser parts of the specimen absorb more electrons, making them look darker on the image you end up with.
TEMs give very high resolution images, to the point where you can see the internal structures of organelles such as chloroplasts. Their limitation is that they can only be used on thin specimens as electrons have to be able to pass through.

32
Q

Scanning electron microscopes
How do they work?
What is their strength and weakness?

A

SEMs scan a beam of electrons across the specimen. This knocks off electrons from the specimen, which are gathered in a cathode ray tube to form an image. The image you end up with show the surface of the specimen and they can be 3D.
SEMs can be used on thick specimen, but their limitation is that the resolution of their images is lower than that of TEMs.

33
Q

How do you prepare a temporary mount of a specimen on a slide?
3 steps

A
  • Start by pipetting a small drop of water onto the slide. Then use tweezers to place a thin section of your specimen on top of the water drop.
  • Add a drop of stain to the specimen. (Stains are used to highlight objects in a cell) (Eosin is used to make the cytoplasm show up. Iodine in potassium iodide solution is used to stain starch grains in plant cells.)
  • Finally, add the cover slip. To do so, stand the slip upright on the slide, next to the water droplet. Then carefully tilt and lower it so it covers the specimen. Try not to get any air bubbles underneath as they may obstruct your view of the specimen.
34
Q

Name the 3 steps of cell fractionation

A

Homogenisation
Filtration
Ultracentrifugation

35
Q

What is the point of cell fractionation?

A

Cell fractionation allows one to separate organelles from the rest of the cell they are inso that they can be observed under an electron microscope.

36
Q

Explain homogenisation

A

Homogenisation is a process used to break up the cells. It can be done by vibrating the cells or grinding the cells up in a blender. This breaks up the plasma membrane and releases the organelles into solution.

37
Q

What are the 3 conditions for homogenisation?

A
  • The solution must be kept ice cold, to reduce enzyme activity and prevent organelle breakdown.
  • The solution must also be kept isotonic, meaning there should be the same concentration of chemicals as the cells being broken down. This prevents damage to the organelles through osmosis.
  • A buffer solution should be added to maintain the pH.
38
Q

Explain the filtration stage of cell fractionation

A

Filtration is a process used to remove the large ell debris or tissue debris, like connective tissue, from the organelles. The homogenised cell solution is filtered through gauze to separate cell debris from the organelles. The organelles are much smaller than the cell debris, so they pass through the gauze.

39
Q

Ultracentrifugation

What is its purpose and what is the process?

A

Ultracentrifugation is a method of separating the different organelles in the remaining solution based on the weight of the organelles.

  • The cell fragments are poured into a centrifuge tube. The tube is put in a centrifuge and spun at a low speed. This separates the heaviest organelles from the mixture (the nuclei). These nuclei are flung to the bottom of the tube where they form a thick sediment known as the pellet. The rest of the organelles stay suspended in the fluid, called the supernatant.
  • The super natant is drained off into a new tube and spun in the centrifuge at a higher speed. Again, the heaviest remaining organelles, this time the mitochondria, are flung to the bottom of the tube to form a pellet. The supernatant, containing the lighter organelles, can be drained off and poured into a new tube to be centrifuged at an even higher speed.
  • This process is repeated at higher and higher speeds until all the organelles are separated out.
40
Q

Name the organelles from heaviest to lightest

Centrifuge order

A
Nuclei
Chloroplasts 
Mitochondria
Lysosomes
Endoplasmic Reticulum
Ribosomes
41
Q

In multicellular organisms, not all cells retain the ability to ________ . The ones that do follow a ___ _____ of which _______ is a part.

A

Divide
Cell cycle
Mitosis

42
Q

What are the four stages of the cell cycle?

A

Gap 1
Synthesis
Gap 2
Mitosis

43
Q

What does interphase consist of?

A

Gap 1, synthesis and gap 2.

Interphase is a period of cell growth and DNA replication.

44
Q

What happens in Gap 1?

A

The cell grows and new organelles and proteins are made.

45
Q

What happens in Synthesis?

A

The cell replicates its DNA, ready to divide by mitosis.

46
Q

What happens in Gap 2?

A

The cell continues growing and the proteins required for cell division by mitosis are made.

47
Q

What happens in Mitosis?

What is the point of mitosis?

A

The parent cell divides to produce two genetically identical daughter cells that contain an exact copy of the DNA of the parent cell.
Mitosis is needed for the growth of multicellular organisms and the repair of damaged tissues.