Cells Flashcards
Maginification
How much bigger an image is from a microscope compared to the specimen.
Resolution.
The quality of the image.
Eg) how well you can distinguish between two points that are close together.
S.E.M
Scanning electron microscope
T.E.M
Transmission electron microscope.
Ultrastructure
The minute structure if a biological specimen ( anything inside a cell)
Wavelength
The distance between two successive crests of a wave.
The equation for magnification
Length of an Image / length of an object.
Cell fractionation
The process where cells are broken up and the different organelles they contain are separated.
What are the three steps of cell fractionation?
- Homogenisation = breaking up of cells
- filtration = getting rid of large pieces of debris.
- ultracentrifugation= separating the organelles.
How does ultracentrifugation work?
Cell fragments poured into the centrifuge.
Spins at low speed; heavy organelles sent to bottom. (Pellets) and are filtered out.
The less dense supernatant is then put back in the centrifuge.
Continue until the desired organelle is the pellet.
Electron
Negatively charged particle
Photomicrograph
Image of a microscopic object. Taken with aid of a microscope.
How does an electron microscope work?
Beams of electrons fired through sample. And lenses.
Shadow image of specimen created.
Difference between TEM and SEM
TEM = higher resolution
= must use v. Thin,
SEM = use on thicker specimen and can be 3D.
= lower resolution;
Pros and cons of SEMs
Pros = use on thicker specimen and can be 3D.
Cons = lower resolution than TEM. & must be dead specimen.
Pros and cons of TEM
Pros = higher resolution
Cons = must use v. Thin, dead specimen
Eye piece graticule
Glass disk in the eyepiece with 1cm scale on it.
=measure size of object. But first must calibrate.
Calibrate
Find the correct magnification by comparing the reading of the graticule to a standard measurement (stage micrometer)
Stage micrometer
Microscope slide with a 2mm long scale unit.
0.01 subdivisions.
Steps to calibrating a graticule.
- Line up graticule to micrometer.
- See how many units on the graticule scale = 10(e.g) on the micrometer.
- Divide each side to show what 1unit on micrometer = on graticule scale.
= 40x magnification.
How can you measure cell size using a graticule?
Find size of image (eg. 7mm = 7000micrometers)
Divide image size by magnification.
Eg. 7000/40x = 175micro m
Ultrastructure
The internal structure of a cell which suits its function.
Eukaryotic cells
Type of cell with specific structure for specific functions.
Have distinct nucleus.
Posses membrane bound organelles.
Nucleus
Holds DNA
Control centre of cell.