cells Flashcards

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1
Q

What are the functions of lysosomes?

A
  • hydrolyse phagocytic cells
  • exocytosis - releases enzymes to the outside of the cell to destroy material
  • completely break down dead cells (autolysis)
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2
Q

What are the functions of Golgi Apparatus?

A
  • secrete carbohydrates
  • form lysosomes
  • transport, modify and store lipids
  • add carbohydrates to form glycoproteins
  • produce secretory enzymes
  • molecules are ‘labelled’ with their destination
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3
Q

What are the functions of mitochondria?

A
  • site of ATP production
  • site of aerobic respiration
  • has the DNA that codes for the enzymes needed for respiration
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4
Q

What are the functions of a nucleus?

A
  • site of DNA replication and transcription (making mRNA)
  • contains the genetic code for each cell
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5
Q

What are the functions of the SER?

A
  • synthesis
  • stores lipids and carbohydrates
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6
Q

What are the two types of electron microscopes?

A
  • scanning electron microscope (SEM)
  • transmitting electron microscope (TEM)
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7
Q

What is the formula for magnification?

A

magnification = size of image / size of object

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8
Q

What are the features of an SEM?

A
  • SEMs knock electrons off the specimen and these electrons come together to form an image
  • SEM images can be three-dimensional
  • Specimens do NOT have to be thin like when using a TEM
  • Resolution is lower than that produced by a TEM
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9
Q

What are the features of a TEM?

A
  • TEMs use electromagnets to focus the electron beam
  • TEMs are high resolution microscopes
  • in thin specimens, you can see the internal structures of organelles such as chloroplasts
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10
Q

What is meant by ‘membrane-bound organelle’?

A

an organelle that is surrounded by a phospholipid layer

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11
Q

Examples of membrane bound organelles

A
  • mitochondria
  • endoplasmic reticulum (SER and RER)
  • chloroplasts
  • lysosomes
  • nucleoli & nuclei
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12
Q

Examples of non-membrane bound organelles

A
  • ribosomes
  • cytoskeleton
  • centrioles
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13
Q

What is meant by magnification?

A

how many times bigger the image is compared to the object

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14
Q

What is meant by resolution?

A

the minimum distance that objects can be in order for them to appear as separate things

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15
Q

Why is a solution cold in homogenisation?

A

so the the rate of enzyme (lysosyme) activity is decreased

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16
Q

Why is a solution buffered in homogenisation?

A

it maintains a constant pH so it does not denature proteins

17
Q

Why is a solution isotonic in homogenisation?

A

has the same water potential as the tissue so no net movement of water by osmosis, so this prevents cell lysis