CELLS Flashcards
What are all of the eukaryotic cells you need to know?
animals plant fungal algal
what are the three parts of the nucleus?
nucleus: nucleolus, nuclear envelope, nuclear pores
What is found in a plant cell’s wall? What does it allow?
Plasmodesmata, channels for exchanging substances between adjacent cells
what can algal cells be that plant cells can’t be?
Algal cells can be unicellular (chlorella) or multicellular (seaweed)
What don’t all plant cells contain?
they don’t all contain chloroplasts
How can chloroplasts sometimes appear in algal cells?
As one large chloroplast instead of several smaller ones
How are fungal and algal cells similar?
fungal cells can also be multicellular (mushrooms) or unicellular (yeast)
What is the cell wall made up of in algal cells?
cellulose
What is the cell wall made up of in fungal cells?
chitin cell wall
What is the cell wall made up of in plant cells?
cellulose cell wall
What surrounds animal cells?
Cell-surface plasma membrane
function of cell-surface membrane
regulates movement of substances in and out of cell, has receptor
molecules on it allowing it to respond to chemicals like hormones
function of nucleus
control’s cell’s activities by controlling the transcription of DNA, pores allow substances to move between the cytoplasm and the nucleus, THE NUCLEOLUS MAKES RIBOSOMES
what is the nuclear envelope
double membrane which contains pores
function of mitochondria
site of aerobic respiration provide ATP, thus provides a source of energy, they are found in high numbers in cells that are very active and require a lot of energy e.g. muscle cells
structure of mitochondria
oval-shaped, double membrane, inner is folded to form structures called cristae, inside is the matrix which contains enzymes involved in respiration
function of chloroplasts
site of photosynthesis, found in algal and plant cells, LDR occurs in the thylakoid membranes, LIR occurs in the stroma
function of golgi apparatus
modifies and packages new lipids and proteins, also makes lysosomes
vesicles often seen at edges of the fluid filled, membrane bound sacs
structure of chloroplasts
double membrane, also contains thylakoid membranes which can be stacked to form grana in some parts of the chloroplast, the grana can be linked together by lamellae, which are thin flat pieces of thylakoid membrane
function of golgi vesicle
proteins/lipids packaged into golgi vesicles and they then transport them by moving towards the membranes and fusing with them releasing contents to target area/ exocytosis
stores lipids/proteins, transports them out of the cell via the cell-surface membrane
function of lysosomes
contains digestive enzymes, lysozymes, they are kept separate from the cytoplasm by the surrounding membrane and can be used to digest invading cells (phagocytosis) or can be used to breakdown worn out components of the cell
function of ribosomes
protein synthesis, found floating free in cytoplasm, prokaryotic ribosomes are smaller than eukaryotic
RIBOSOMES ARE MADE UP OF PROTEINS AND RNA (rRNA)
function of RER
The RER’s surface is covered witg ribosomes
folds and processes proteins that have been made at the ribosomes
functions of SER
no ribosomes,
synthesises and processes lipids
function of cell wall
provides structural support for the cell, prevents cell from changing shape, it is a rigid structure that surrounds plant/algal/fungal cells
function of cell vacuole
helps to maintain pressure inside the cell and keep the cell rigid, stops the plant wilting, involved in the isolation of unwanted chemicals inside the cell
structure of the cell vacuole
membrane that surrounds vacuole is called the tonoplast, it’s a membrane-bound organelle, it contains cell sap which is a weak solution of sugar + salts
what do prokaryotic cells not contain that eukaryotes do?
membrane-bound organelles
what is a cell wall made of in a prokaryotic cell?
murein cell walls
what is murein?
A glycoprotein, found in prokaryotic cell walls, a glycoprotein is a protein with a carb attached
NOT ALL PROKARYOTES HAVE A…
flagellum
feature of all porkaryotic cells that are not features of eukaryotic cells
DNA is not associated w/ histones, whereas eukaryotic they are
circular loop of DNA
DNA free in cytoplasm
No membrane-bound organelles
Murein/peptidoglycan in cell wall
what is the slime capsule’s function?
to protect the bacteria from attack by cells of the immune system
Binary fission:
circular DNA and plasmids replicate
cell gets bigger and DNA moves to opposite poles of the cell
cytoplasm begins to divide
new cell walls begin to form
cytoplasm divides and two new daughter cells produced, each have a copy of the circular DNA and varied numbers of copies of the plasmids
What are viruses?
ACELLULAR
just nucleic acids surrounded by protein
No cell-surface membrane, no cytoplasm, no ribosomes.
Strucutre of virus
Capsid - protein coat which protects genetic material
Attachment proteins which bind to receptors on a cell
They contain nucleic acids/genetic material which codes for viral proteins
Why are virsuses classed as acellular/non-living?
No cell-surface membrane
Not made of cells
Have no metabolism/no metabolic reactions
Cannot independently move/respire/replicate/excrete
Have no nutrition
How does a virus replicate once already inside the cell?
RNA converted into DNA using reverse transcriptase
DNA inserted into helper T cell’s/or other cell’s nucleus/DNA
DNA transcribed into viral/HIV mRNA
HIV mRNA translated into new HIV/viral proteins
How do viruses bind to host cells?
They use their attachment proteins to bind to the complementary receptor proteins on the cell-surface membrane of a host cell
Why can some viruses only infect one type of cell?
Their attachment proteins are only specific to the receptor proteins found on the cell-surface membrane of one type of cell
How does HIV replicate that is slightly different to other viruses?
HIV releases its capsid into the host cell as well as its genetic material
Conversion used in magnification calculations:
x 1000 x 1000
Mm –> Um –> Nm
/ 1000 /1000
What is resolution?
The ability of a microscope to be able to distinguish between two objects that are close together
If a microscope cannot distinguish between two objects then increasing the mag won’t help
A microscope cannot distinguish between objects that are smaller than its max. resolution
Tell me about optical microscopes:
Use light to form an image
Max resolution of about 0.2um
Can’t use opticals to view organelles smaller than that e.g. ribosomes, endoplasmic reticulum, lysosomes.
Possibly make out mitochondria
max mag = x1500
Electron microscopes:
Use electrons to form an image
Higher reso than optical
more detailed images
max reso. 0.0002um
max useful mag. x1 500 000
Produce black and white images, often coloured by a computer
TEMS
use electromagnets to focus beams of electrons, which is then transmitted through the specimen
Denser parts absorb more electrons - look darker on image
TEMS give high resolution images
can see internal structures of organelles
Need to view specimen in a vacuum - so cannot look at living organisms
Only used on thin specimens
SEMS
Scan a beam of electrons across the specimen
Knocks off electrons from the specimen which are gathered in the cathode ray tube to form an image
Images show the surface of the specimen and can be 3D
Can be used on thick specimens
Give lower resolution images than TEMS
Can only be used on non-living specimens
Differences between optical microscope and TEM
TEM CANNOT produce coloured images (optical CAN), highER/greatER resolution, however only on non-living/dehydrated organisms and specimen has to be thinNER (contrast)
TEM focuses a beam of electrons onto the specimen using electromagnets whereas optical uses light and uses lenses to focus to form an image
Denser parts absorb more elctrons, appear darker on image
How do you prepare a microscope slide?
Done using a temporary/wet mount where the specimen is suspended in a drop of liquid e.g. water/oil
Pipette a small drop of water onto the centre of the slide
Use tweezers to place thin section of your specimen on top of water droplet
Add a drop of stain
Add cover slip, stand slip upright on the slide next to the droplet, carefully tilt and lower it so it covers the specimen, avoid air bubbles as they’ll obstruct view of specimen.
artefacts
not part of the specimen
usually created during the preparation of a specimen, dust air bubbles fingerprints, obstruct view of specimen, innacucuracies caused by squashing/staining sample
How to make a temporary mount of plant tissue to identify position of starch grains:
Add a drop of water oj the glass slide
Obtain a thin section and place onto a glass slid, float on a drop of water
Stain with iodine dissolved in potassium iodide solution in order to identify the starch
Lower the cover slip slowly onto the specimen using a mounted needle and squash
What stains can you use in the preparation of a microscope slide?
Iodine in potassium iodide to stian starch
Eosin used to make cytoplasms show up
Why do you need to squash the sample on a microscope slide?
In order to spread out the cells, evenly distributed on the slide, in order to allow light to pass through to the specimen can be seen properly/clearly
Order of organelle separation during ultracentrifugation:
Nucleus (chloroplasts) mitochondria, lysosomes, endoplasmic reticulum, ribosomes
Isotonic, buffer, ice cold
no osmotic effect/prevent lysis/bursting of organelle
keeps pH constant so it doesn’t denature any enzymes
prevent any enzyme activity so the organelles are not broken down
why do we filter during cell fractionation?
remove cell debris or tissue debris such as connective tissue from the organelles
What does the cell cycle consist of?
A period of cell growth and DNA replication called interphase
And a period of cell division called mitosis
three stages of interphase
gap phase 1 - cell grows + new organelles and proteins made
Synthesis - DNA replication, ready to divide by mitosis
gap phase 2 - cell keeps growing and proteins needed for cell division made
then mitosis
What happens during interphase
DNA unravels and replicated as well as organelles being replicated and ATP CONTENT IS INCREASED
Mitsosis - INPROMEANTE
I - DNA (unravelled)/organelle replication
N - ATP content increased
P - DNA condenses
R - Centrioles move to opposite poles of the cell
O - Nuclear envelope breaks down, chromosomes free in cytoplasm
M - Chromosomes line up at equator of cell
E - Spindle attaches to the centromere
A - Centromeres divide
N - Spindles contract, get shorter pulling sister chromatids to opposite poles - V shaped
T - Cytokinesis finishes off in telophase (started in anaphase)
E - Chromatids uncoil/unwind once at pole, become long and thin and called chromosomes again
TWO GENETICALLY IDENTICAL DAUGHTER CELLS
What change to environmental conditions can increase the growth rate of the cells?
Increase temp –> increased enzyme activity
Increase glucose/O2 –> increased respiration
Increased conc of phosphate –> Increased ATP/DNA/RNA
Increased conc of nucleotides –> Increased DNA synthesis