Cells Flashcards
Structure of Nucleus
- Nuclear envelope: double membrane surrounding nucleus
- Nuclear pores: allow the passage of larger molecules (e.g. mRNA), out of the nucleus.
- Nucleoplasm: granular, jelly-like material making up the bulk of the nucleus.
- Chromosomes: protein-bound, linear DNA.
- Nucleolus: small spherical region(s) in nucleoplasm. Manufactures ribosomal RNA and assembles ribosomes.
Function of the nucleus
- Controls cell’s activities - Controls entry and exit of materials, and contains nuclear reactions.
- Retains genetic material in the form of DNA and chromosomes.
Structure of Mitochondria
- Double membrane surrounding organelle - controls entry and exit of material.
- Cristae - extensions of the inner membrane, providing a large surface area for the attachment of enzymes and other proteins during respiration.
- Matrix - contains proteins, lipids, ribosomes and DNA matrix contains enzymes for cell respiration.
Function of the Mitochondria
site of aerobic respiration
Structure of Chloroplasts
Chloroplast envelope - double plasma membrane, highly selective
- Grana - stacks of disc-shaped thylakoid membrane.
- Thylakoids - contain chlorophyll used in photosynthesis, can be linked by lamellae to other grana.
- Stroma - fluid-filled matrix that contains starch grains.
Functions of Chloroplasts
site of photosynthesis
Structure of Endoplasmic Reticulum
Rough and Smooth ER have folded membranes called cisternae
RER have ribosomes on the cisternae
Function of endoplasmic reticulum
RER – protein synthesis
SER- synthesises and stores lipids and carbohydrates
Structure of Golgi Apparatus
Flattened sacs and folded membranes making cisternae
Function of Golgi Apparatus
Processes, packages and modifies proteins
Stores lipids and proteins and transports them out of the cell
Vesicle- pinches off cisternae and the modified molecule can be transported.
Structure of Lysosomes
Vesicles/Bags of digestive enzymes
Functions of Lysosomes
Hydrolyse phagocytic cells
Completely break down dead cells (autolysis)
Exocytosis- release enzymes outside of cell to destroy material
Digest worn out organelles for the reuse of materials
Structure of Ribosomes
Small organelle made up of proteins and rRNA (ribosomal RNA)
Eukaryotic cells have large ribosomes
Prokaryotic cells, mitochondria and chloroplasts have smaller ribosomes
Functions of Ribosomes
Carry out protein synthesis
Structure of Cell Wall
Plants- made of microfibrils of cellulose
Fungi- made of chitin, a nitrogenous polysaccharide
Function of Cell Wall
Provides structural strength to the cell
Structure of Vacuoles
- Fluid-filled sac bounded by a single membrane.
- Single membrane around it called tonoplast.
Function of vacuole
Makes cells turgid and therefore provides support
Temporary store of sugars and amino acids
The pigment may colour petals to attract pollinators
Define Tissue
Give an example
A collection of similar cells that work together to perform a specific function.
Example = epithelial tissue - consists of sheets of cells, lining the surfaces of organs, often having a protective or secretory function.
Define eukaryotic cell
A larger cell with a true nucleus that is bounded by a nuclear membrane/nuclear envelope.
Define prokaryotic cell
A smaller cell which has no true nucleus or nuclear envelope. Organelles are not membrane bound
Can you describe how prokaryotic cells differ from eukaryotic cells?
Prokaryotic cells:
- have no nucleus
- smaller 70S ribosomes
- cytoplasm lacks membrane-bound organelles
- much smaller cell
- cell wall contains murein (a glycoprotein)
- plasmids may be found in prokaryotic cells
Eukaryotic Cells:
- have nucleus
- 80S ribosomes
- membrane-bound organelles
- larger cell
- cell wall made of cellulose or chitin
- no plasmids
Can you list other features of prokaryotic cells?
Prokaryotic cells can have:
- one or more plasmids
- a slime capsule surrounding the cell
- one or more flagella
What is cell fractionation?
A method used to isolate different organelles so they can be studied. This enables the individual organelle structures to be studied in an electron microscope
What are the steps of cell fractionation
- Homogenisation
- Filtration
-Ultracentrifugation
Why should the solution be isotonic?
water potential is the same as the solution which prevents osmosis that could shrink or burst organelles
Why should the solution be buffered?
keeps pH constant and avoids damaging the protein structures of the organelle)
Why should the solution be ice cold?
reduces enzyme activity that might damage organelles
How does an optical microscope work?
A beam of light is condensed to create the image as visible light passes and is bent through the lens system to enable the user to see the specimen
What are the properties of an optical microscope?
- the specimen can be alive
- Individual cells are transparent, and their components are not distinguishable unless they are coloured with special stains.
Staining usually kills the cells.
Small organelles are not visible, but living samples can be examined and a colour image is obtained
What are the uses of an optical microscope?
Most student microscopes are classified as light microscopes.
Max resolution is 0.2 micrometres.
The nucleus and mitochondria can be seen with a light microscope.
The max magnification is around x1,500.
How is an electron microscope used?
A beam of electrons that are condensed using electromagnets creating the image. This allows higher magnification and higher resolving power, allowing more detail to be seen with black and white images
Samples must be in a vacuum and non-living
What are the uses of an electron microscope?
Max resolution of 0.0002 micrometres. Around1000 times more than light microscopes.
Max magnification is around x1,500,000.
What are the two types of electron microscopes?
transmission (TEM) and scanning (SEM) electron microscopes.
How does an SEM work?
A beam of electrons moves back and forth across a cell’s surface, creating details of cell surface characteristics.
SEMs knock electrons off the specimen and these electrons come together to form an image.
SEM images can be three-dimensional.
What are the properties of an SEM?
-The specimens do not need to be thin, as the electrons are not transmitting through. -Can produce a 3D image
How does a TEM work?
Extremely thin specimens are stained and placed in a vacuum
The electron beam penetrates the cell and provides details of a cell’s internal structures.
TEMs use electromagnets to focus the electron beams and are high resolution microscopes.
In thin specimens, you can see the internal structures of organelles such as chloroplasts.
What are the steps in calibrating the eyepiece graticule?
Line up the stage micrometre and eyepiece graticule whilst looking through the eyepiece
Count how many divisions on the eyepiece graticule fit into one division on the micrometre scale
For example, each division on the micrometre is 10, so this can be used to calculate one division on the eyepiece graticule at that current magnification. 10/2=5 micrometres
Can you describe the pros and cons of optical microscopes?
Pros:
- cheap
- images in colour
- no training required
- live specimens
Cons:
- low magnification x1500
- low resolution
- 2D images
Can you describe the pros and cons of transmission electron microscopes?
Pros:
- high resolution images
- high magnification
- visible internal structures
Cons:
- expensive
- training is required
- no colour images
- 2D images
- only thin specimens
Can you describe the pros and cons of scanning electron microscopes?
Pros:
- 3D images
- high magnification
- high resolution
- thick specimens
Cons:
- expensive
- training is required
- no colour images
How do you prepare a slide for an optical microscope?
- Pipette a drop of water onto the slide
- Use tweezers to place a thin section of your specimen on top of the droplet
- Add a drop of a stain
- Add a cover slip - remove all air bubbles
What is the difference between magnification and resolution?
MAGNIFICATION - increasing the size of an image. Up until the limit of resolution, an increase in magnification = an increase in detail.
RESOLUTION = minimum distance apart that two objects can be for them to appear as separate items.
Can you describe the principles of cell fractionation and ultracentrifugation in separating cell components?
- Homogenisation
- tissue is broken up in a cold, isotonic buffer solution to release the organelles into a solution - Filtration
- the homogenised cell solution is filtered through a gauze
- this separates any large cell debris - Ultracentrifugation
- the cell fragments are poured into a test tube and placed in a centrifuge and spun at a low speed
- a thick sediment - the pellet - is at the bottom of the tube and the fluid above is the supernatant
- the supernatant is drained into a new tube and spun again at a higher speed
- a new pellet forms and again, the supernatant is drained off and spun again at an even higher speed
- this process is repeated at higher speeds each time until all the organelles are separated out
How are the organelles separated out during centrifugation?
They are separated in order of mass and the order is usually:
- nuclei
- mitochondria
- lysosomes
- endoplasmic reticulum
- ribosomes
Why are virus cells not considered living?
They have no nucleus, plasma membrane, cytoplasm, or ribosomes.
They are not made of cells.
They cannot reproduce independently
How do viral cells replicate?
Viruses replicate by injecting their nucleic acid into a host cell:
Can you describe the structure of virus particles?
no plasma membrane, cytoplasm, ribosomes
- a core of genetic material : DNA or RNA
- capsid surrounding core
- attachment proteins around edge of capsid that are complementary to specific host cells
Outline the differences between mitosis and meiosis.
Mitosis results in 2 genetically identical diploid daughter cells.
Meiosis results in 4 genetically different haploid daughter cells.
List the stages of mitosis
Prophase
Metaphase
Anaphase
Telophase
outline interphase
Precedes Mitosis
- Cell is not dividing.
- Considerable cellular activity - replication of DNA, two copies on centromere.
outline prophase
- Chromosomes become more visible, thicken.
- Centrioles move to opposite ends of the cell (poles).
- Spindle fibres develop from each of the centrioles (spindle apparatus).
outline metaphase
- Chromosomes seen to be made up of two chromatids.
- Microtubules attach to centromere - chromosomes pulled to the cell equator where they line up.
Outline Anaphase
- Centromeres divide, separating each pair of sister chromatids.
- Chromatids pulled to their respective poles as spindles contract, centromeres first - v-shaped.
Outline Telophase
- Chromosomes reach their opposite poles and then uncoil, become long and thin again
- Spindle fibres disintegrate; nuclear envelope and nucleolus reform.
Why is mitosis so important?
GROWTH - ensures that all cells growing from original cell of an organism are genetically identical.
REPAIR - Important that replacement cells produced have an identical structure and function to lost cells.
REPRODUCTION - Single-celled organisms divide by mitosis to give 2 new organisms - each new organism is genetically identical to the parent organism.
How does cancer arise?
Result of gene damage controlling mitosis and cell cycle.
Mutant cells are structurally and functionally different - most mutant cells die but surviving mutant cells become tumours.
Outline cytokinesis
the cytoplasm is divides forming two genetically identical cells
Animal cells: a ‘cleavage furrow’ forms and separates the daughter cells
Plant cells: a ‘cell plate’ forms at the site of the metaphase plate. Once the cell plate reaches the cell walls of the parent cell, new cell walls are produced, separating the new daughter cells
Outline the processes that occur during interphase
G1- growth of organelles
S- DNA Synthesis
G2- Growth and preparation for mitosis
Difference between benign and malignant?
Malignant - grow rapidly, spread, more likely to be life-threatening.
Benign - grow more slowly, do not spread less likely to be life-threatening
How can cancer be treated?
Involves killing dividing cells by blocking a part of the cell cycle - cell division and therefore cancer growth ceases.
Chemo disrupts cell cycle by preventing DNA replication or by inhibiting the metaphase stage of mitosis by interfering with spindle formation.
Outline the process of Binary Fission
- The circular DNA in the cells replicates and both copies attach to the cell membrane.
Plasmids also replicate. - The cell membrane then begins to grow between the two DNA molecules and begins to pinch
inwards, dividing the cytoplasm in two. - A new cell wall forms between the two DNA molecules dividing the original cell. The identical
daughter cells each have a single copy of the circular DNA and a variable number of copies
of the plasmids
Outline role of phospholipids in cell-surface membrane structure.
Their hydrophilic/hydrophobic interactions lead to the formation of a phospholipid bilayer.
- Allow lipid-soluble substances to enter/exit cell.
- Prevent water-soluble substances entering and leaving cell.
- Make the membrane flexible and self-sealing.
Outline role of proteins in cell-surface membrane structure.
- to aid movement across the
membrane - provide mechanical support
- act in conjunction with glycolipids as
receptors.
Outline role of glycolipids in cell-surface membrane structure.
- acts as a cell surface receptors for
certain molecules - allow cells to
adhere to one another to form tissues.
Outline the role of glycoproteins in cell-surface membrane structure
- carbohydrates that attach to extrinsic proteins and acts
a cell surface receptors and
neurotransmitters. - allow cells to recognise one another as well as attach to
form tissues.
Comment on the permeability of the cell-surface membrane.
Controls the movement of substances into/out of the cell.
Most molecules don’t freely diffuse across it because many are:
- Not lipid-soluble
- Too large to pass through
- Same charge as protein channel charges - repelled even if small
- Charged/polar - can’t pass through the non-polar hydrophobic tails in the phospholipid bilayer.
Explain the “fluid-mosaic” model of the cell-surface membrane structure.
The “mosaic” term of this model refers to the mixture of lipids and intrinsic proteins in the membrane. These boundaries are also “fluid” because their components can move flexibly
Define Diffusion
the net movement of particles from an area of high concentration to low concentration until an even distribution is maintained.
How does concentration gradient affect diffusion?
the greater the concentration gradient, the faster the rate of diffusion.
How does membrane thickness affect diffusion?
The thinner the membrane, the faster the rate of diffusion and thin membranes reduce the distance that particles have to travel
How does surface area affect diffusion?
The larger the surface area, the faster the rate of diffusion and increasing the surface area means more particles can be exchanged in the same amount of time.
How does temperature affect diffusion?
The higher the temperature, the faster the rate of diffusion and particles will have more kinetic energy so will be able to move around faster
Give an example of a molecule which can use simple diffusion to pass through the membrane
small, non polar e.g. oxygen and carbon dioxide
What is facilitated diffusion?
passive transport that uses specialized proteins, such as channel proteins and carrier proteins, to help large molecules move across a cell membrane faster
What are channel proteins?
a protein that allows the transport of specific substances across a cell membrane.