Cell theory 3 Flashcards
Indirect active transport example and explanation
sodium-glucose cotransporters move Na+ and glucose together into the cell – Na+ moves down its C gradient, releasing ATP in the process, and glucose uses this ATP to move against its C gradient. This type of transport depends on the Na+ pump to pump ions back out and maintain the sodium gradient (kidneys during reabsorption of glucose and in the small intestine during the absorption of glucose from digested foods)
How does the cell wall protect a cell in hyper and how in hypotonic environments?
can only protect the cell in a hypotonic environment by keeping it from bursting (it maintains its shape and keeps it turgid) but in hypertonic, the plasma membrane simply detaches from the cell wall and the cell shrinks as it dehydrates (together with its vacuole)
Plasmolysis and deplasmolysis
when water leaves a cell in a hypertonic environment so it shrinks, the plasma membrane detaches from the cell wall, and the vacuole dehydrates – deplasmolysis is when water renters the cell that went through plasmolysis (the cell’s volume increases and the membrane attaches to the cell wall)
What are three mechanisms for preventing the cell from bursting in a hypotonic solution?
1| Cell wall protection
2| Using contractile vacuoles (E from ATP used to pump ions into the CV so that the water from the cytoplasm enters – CV merges with the plasma membrane and water is removed by exocytosis)
3| Maintaining the concentration of the extracellular fluid (multicellular organisms – kidneys)
Water potential
a potential F held by water per unit of volume maximal in pure water (0 kPa) because the effective number of water molecules is reduced when it is bound to solute molecules (can’t move freely then) – depends on solute potential and pressure potential (the higher the hydrostatic pressure, the higher the WP) – water moves from higher WP to lower WP
Endocytosis and exocytosis
the active transport of large particles or entire cells across the membrane by means of vesicles possible due to the fluidity of the membrane – in endocytosis, vesicles are brought to lysosomes where the enzymes digest the engulfed substances
Freshwater vs ocean fish (type of environment which affects whether water goes out or in, volume and concentration of the urine, which way active transport occurs and whether fish have to drink water)
freshwater: hypotonic environment, water into, large urine V, diluted urine, active transport into, and doesn’t drink water
vice versa for ocean fish
What are morphogens and how do they perform their function?
chemical signals released by some cells during the blastocyst stage that start the cell specialization process in an embryo – get released and diffuse and cells specialize according to what concentration of morphogens they are exposed to (morphogen gradient indicates to a cell its position in the embryo) because they impact gene expression
Stem cells and different types
cells with the ability to divide endlessly and differentiate along different pathways – totipotent, pluripotent and multipotent (tissue specific)
Differentiate between different STEM cell types
totipotent can become any cell type in the body (body and placenta), have all genes switched on/expressed – pluripotent can become any type of cell in the body but not the placenta (in the blastocyst, the inner cell mass) – multipotent can develop into cells within a narrow group like liver, skin, and hematopoietic SC in bone marrow (regeneration of adult tissues)
What is SC niche?
the precise location of SC in the organism – place where SC can either remain inactive for a long period or proliferate and rapidly differentiate (these are determined by the microenvironment of the niche) – exist only for multipotent SC (bone marrow and hair follicles)
What does cell V determine and what does cell SA determine, what happens when V exceeds the SA capacity?
V determines the rate of metabolism (the amount of substances transported in and out of the cell)
SA determines the effectiveness of the exchange of materials
the cell dies (SA/V ratio too small) or, to prevent that, divides
Ligand and describe ligand action
a molecule that binds selectively to a specific site on another molecule released by a source and affecting target cells – binds to the receptors on the target cell and causes changes in the receptor’s conformation which is recognized by other processes in the cell, the signal is passed on and the cell changes its behavior (metabolic activity)
Signal transduction pathway and what are two types according to two different types of ligand receptors?
the sequence of interactions in cells triggered by ligand binding – transmembrane STP (on the cell’s surface) and intracellular STP (in the cell cytoplasm)
Transmembrane STP steps
1| Reception – binding causes a reversible conformational change of the receptor
2| Signal transduction – the newly catalytically active receptor causes the production of a secondary messenger within the cell
3| Response - activation of cellular responses – signal is carried by the secondary messenger to effectors that carry out the response
Intracellular STP steps
1| Steroid hormone enters the cell (hydrophobic)
2| Hormone-receptor complex formed
3| Complex travels to the nucleus where it binds to the promotor region on the DNA and directly controls the gene expression process
The requirements for a signaling chemical
1) Has a distinctive shape and chemical properties so the receptor can distinguish between it and other chemicals
2) Is small and soluble enough to be transported
Different types of ligands
hormones, neurotransmitters, cytokines and Ca ions