Cell: The Unit Of Life Flashcards

1
Q

What was the contribution of Matthias Schleiden to Cell Theory?

A

1838, German botanist, studied plant cell and stated that all plants are made of cells and their products

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2
Q

What was the contribution of Theodore Schwann to Cell Theory?

A

1839,
studied animal cell, reported presence of plasma membrane,
studied plant cell and discovered cell wall
proposed that bodies of animals and plants are composed of cells and their products

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3
Q

What was the contribution of Rudolf Virchow to Cell Theory?

A

1855,
explained that new cells are formed from pre-existing cells- Omnis cellula-e cellula

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4
Q

What is the size of:
1. RBCs
2. Bacteria
3. Mycoplasma
4. PPLO

A
  1. 7 micron
  2. 3 - 5 micron
  3. 0.1 - 0.3 micron
  4. less than 0.1 micron
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5
Q

What is glycocalyx composed of?

A

polysaccharides, may sometimes contain proteins

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6
Q

What is the cell wall of prokaryotes composed of? How is it affected by penicillin?

A

It is composed of peptidoglycan(murein or mucopeptide)
Penicillin inhibits cross linking of peptidoglycan strands, cause cell to undergo lysis

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7
Q

What are the functions of mesosome?

A

Cell wall formation, DNA replication and distribution to daughter cells, respiration, secretion

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8
Q

What is structure of flagellum in prokaryotes? What is its function?

A

basal body, hook, filament(made of flagellin protein)
function: locomotion and movement

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9
Q

Describe the process of gram staining.

A

Bacterial smear
Air drying
Stain with crystal violet
Stain with iodine(mordant)- formation of CVI complex
Treatment with alcohol(destaining)
Counter staining with safranin

blue stained - gram positive
pink stained - gram negative

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10
Q

Give three examples of bacteria that have chromatophore.

A

Cyanobacteria (blue green algae) - Nostoc, Anabaena
Green sulfur bacteria
Purple sulfur bacteria

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11
Q

What are pili made of? In what type of bacteria are they usually found? What is their function?

A

made of protein pilin
found in gram-negative bacteria
function: formation of conjugation tubule to allow transfer of genetic material between compatible bacteria

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12
Q

What is plasmid?

A

Plasmid is extrachromosomal, ds circular DNA present in some bacteria.

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13
Q

Flagellum is attached to ____.

A

Cell wall

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14
Q

Where are ribosomes found in prokaryotes? What is their function in each location?

A

cytoplasm and plasma membrane
in cytoplasm - they synthesize proteins which remain within the cell
in plasma membrane - they synthesize proteins which are transported out

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15
Q

What is a polysome? What is its function?

A

When several ribosomes attach to a single mRNA they form a chain called polysome.
Ribosomes of a polysome translate the mRNA into proteins.

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16
Q

What is the percentage of proteins and lipids in plasma membrane of human RBC?

A

52% proteins, 40% lipids

17
Q

What does plasma membrane of eukaryotes consist of?

A

Phospholipid bilayer, proteins, carbohydrates and cholestrol

18
Q

On the basis of ease of extraction, biomembrane proteins are classified as:

A

integral and peripheral

19
Q

State the composition of cell wall in fungi.

A

it is composed of chitin, a polymer of NAG (N-acetylglucosamine)

20
Q

State the composition of cell wall in algae.

A

It is composed of cellulose, galactans, mannans and minerals like calcium carbonate.

21
Q

State the composition of cell wall in plants.

A

cellulose, hemicellulose, lignin, pectin and suberin

22
Q

What is middle lamella? What is it made of? What is its function?

A

thin sticky amorphous layer of cementing material
made of calcium and magnesium pectate
holds adjacent cells together in plant tissue

23
Q

what is genomic DNA of prokaryotes?

A

single chromosome, circular ds DNA