Cell Surface Markers/IHC

Question 0

Vimentin stains…

Answer 0

Mesenchymal cells

Question 1

Identifies what cell types? Flow, histo, or both?

CD21

Answer 1

Mature B cells

Flow and histo

Question 2

Cytokeratin stains…

Answer 2

Epithelial cells

Question 3

Co-staining with vimentin and cytokeratin can occur in… (Name 5 tumors)

Answer 3

Mesothelioma
Synovial cell sarcoma
Anaplastic sarcoma
Carcinosarcoma
Melanoma

Question 4

S100 stains…

Answer 4

Neural crest origin, including
Adipocytes
Chondrocytes
Melanocytes

Question 5

GFAP stains…

Answer 5

Peripheral nerve sheath tumors

Astrocytes

Question 6

MelanA stains…

Answer 6

Melanoma

Question 7

Actin stains…

Answer 7

Muscle origin cells

Question 8

Desmin stains…

Answer 8

Muscle origin cells

Question 9

SMA stains

Answer 9

Smooth muscle cells

Question 10

Myoglobin stains…

Answer 10

Skeletal and cardiac muscle

Question 11

Factor XIII stains…

Answer 11

Vessel origin cells

Question 12

Synaptophysin stains…

Answer 12

Neuroendocrine origin

Question 13

Chromogranin stains…

Answer 13

Neuroendocrine origin

Question 14

Thyroglobulin stains…

Answer 14

Follicular thyroid cells

Question 15

Calcitonin stains

Answer 15

Parafollicular thyroid cells

Question 16

Identifies what cell types? Flow, histo, or both?

CD3

Answer 16

T cells (mature)

Flow and histo

Question 17

Identifies what cell types? Flow, histo, or both?

CD79a

Answer 17

B cells (early)

Flow and histo

Question 18

MUM1 stains…

Answer 18

Plasma cells

Question 19

Identifies what cell types? Flow, histo, or both?

CD18

Answer 19

Histiocytic cells

Flow and histo

Question 20

Uroplakin stains…

Answer 20

Prostatic and urothelial

Question 21

cKit stains… (And give CD alias)

Answer 21

CD117
Mast cell tumors
GIST

Question 22

Identifies what cell types? Flow, histo, or both?

CD45

Answer 22

All leukocytes

Flow only

Question 23

Identifies what cell types? Flow, histo, or both?

CD34

Answer 23

Precursor/stem cells

Flow only

Question 24

Identifies what cell types? Flow, histo, or both? Ig

Answer 24

B cell (early and mature) Flow, maybe histo?

Question 25

Identifies what cell types? Flow, histo, or both? CD3e

Answer 25

T cell (early) Flow only

Question 26

Identifies what cell types? Flow, histo, or both? CD3

Answer 26

T cell (mature) Flow and histo

Question 27

Identifies what cell types? Flow, histo, or both? CD4

Answer 27

Neutrophil Flow and frozen sections

Question 28

Identifies what cell types? Flow, histo, or both? CD8

Answer 28

T cell (mature) Flow and frozen sections

Question 29

Identifies what cell types? Flow, histo, or both? CD11c

Answer 29

Dendritic cells Flow and frozen sections

Question 30

Identifies what cell types? Flow, histo, or both? CD11d

Answer 30

LGL Flow and frozen sections

Question 31

Identifies what cell types? Flow, histo, or both? CD14

Answer 31

Monocytes Flow only

Question 32

Identifies what cell types? Flow, histo, or both? CD41

Answer 32

Platelets, megakaryocytes Flow. Histo?

Question 33

Mechanism of action of erythropoietin?

Answer 33

Binds to Epo receptor on RBC precursors in bone marrow and prevents apoptosis.