cell structure and composition Flashcards

membrane proteins and transport: recall the functions of membrane proteins, and distinguish between simple diffusion, facilitated diffusion and active transport of ions and molecules across cell membranes, recall specific examples of each

1
Q

two forms of movement of molecules through membranes (energy)

A

active and passive

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2
Q

what does active transport use

A

ATP-coupled proteins

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3
Q

3 examples of passive transport

A

osmosis, simple and facilitated diffusion

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4
Q

feature of simple diffusion

A

down conc. gradient

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5
Q

features of facilitated diffusion

A

down conc. gradient, through protein channels (with water as charged)

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6
Q

define symporter

A

protein channel which moves two or more molecule types across the membrane in the same direction

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7
Q

define antiporter

A

protein channel which moves two or more molecule types across the membrane in the opposite direction

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8
Q

3 examples of active transport

A

phagocytosis, pinocytosis, exocytosis

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9
Q

define phagocytosis

A

engulfment by membrane of EC objects such as bacteria, cell debris, which end up in IC vesicles

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10
Q

define pinocytosis

A

engulfment by membrane of EC solute which ends up in IC vesicles

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11
Q

define exocytosis

A

movement of proteins and other molecules, such as hormones and blood clotting factors, from IC vesicles towards EC space by fusion with cell membrane

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12
Q

what is the process which provides evidence for the fluid mosaic model

A

freeze-fracturing

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13
Q

why do some transmembrane proteins have low mobility

A

membrane skeleton, actin containing ‘fences’

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14
Q

proportion of transmembrane proteins in plasma membrane, inner mitochondria membrane and myelin sheath by increasing %

A

myelin sheath < plasma membrane < inner mitochondria membrane

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15
Q

why are the proportions of proteins in different organelles or cells different

A

different functions

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16
Q

4 functions of transmembrane proteins

A

transport, receptors, cell recognition and adhesion, electron carrier

17
Q

why does a steady state form between two ion concentrations

A

chemical conc. gradient and electrostatic force balance

18
Q

what prevents steady state forming between Na+ and K+

A

Na+/K+ ATPase ion channel

19
Q

how many polypeptides present in the Na+/K+ ATPase ion channel

A

2

20
Q

what type of ion channel is the Na+/K+ ATPase ion channel

A

electrogenic antiporter

21
Q

process of Na+/K+ ATPase ion channel transport

A

Pi bound to protein → conformational change → 2 K+ bind EC → Pi released → K+ released IC → 3 Na+ bind IC → ATP hydrolysed to ADP and Pi → return to normal shape → Na+ released EC → Pi remains bound

22
Q

outcome on potential of cell

A

more negative as more +ve charges leave than enter

23
Q

what equation measures membrane potential

A

Nernst equation

24
Q

what is the Na+/K+ ATPase ion channel coupled with in the kidney proximal tubule

A

symporter protein

25
Q

what is absorbed in the kidney proximal tubule

A

glucose and amino acids

26
Q

by what mechanism does the absorption of glucose and amino acids function

A

“flip-flip”

27
Q

process of absorption of glucose and amino acids in kidney proximal tubule

A

Na+ moves out of cell via Na+/K+ ATPase antiporter → Na+ binds to glucose → both enter via symporter (facilitated diffusion)

28
Q

example of a protein in cell signalling

A

G-protein coupled receptor

29
Q

what does the G-protein coupled receptor activate

A

secondary messengers

30
Q

3 types of cell signalling

A

exocytosis (e.g. peptide hormones), lipid-soluble molecules that cross membrane, trans-membrane receptors