Cell structure Flashcards
What’s the equation for Magnifacation?
size of image/size of object
Define object.
Material under microscope.
Define Image.
Appearence of material under microscope.
What’s the equation for the size of the object?
Size of Image/ Magnifacation.
Define Resolution.
Minimum distance apart two objects can be, in order for them to appear as seperate items.
What does resolution positively correlate with?
Image clarity.
Why after a certain point does the magnification of a microscope not increase the resolution?
The Microscope has reached it’s limit of resolution.
What is cell fractionation?
Where cells are broken up and organelles iscolated.
What happens before cell fractionation and why?
Tissue is placed in cold buffered solution with the same water potential it has.
Cold- Reduces risk of enzymes breaking down organelles.
Same water Potential- Prevents organelles shrinking, or undergoing lysis via osmosis.
Buffered- So PH does not fluctuate (Change) which stops there being a change of function in organelles and enzymes.
