Cell Structure Flashcards
What is Cell Fractionation?
the process where
- cells are broken up
- different organelles they contain are separated out based on size/mass using gravity
The 3 Steps of Cell Fractionation.
1) homogenisation
2) filtration
3) ultracentrifugation
Define what is meant by magnification.
Magnification - how many times bigger the image is compared to the actual size of a speciman
Define what is meant by resolution.
Resolution - how clearly you are able to distinguish between two points close together on an object
What is homogenisation?
When cells are broken up by homogeniser (blender), releasing organelles from the cell.
- makes a homogenate.
What is filtration?
When the homogenate is filtered to get rid of any large pieces of debris.
What is ultracentrifugation?
The process where fragments in filtered homogenate are separated in a centrifuge - which spins the tube at very high speeds.
- the heaviest organelles are forced to the bottom, forming pellets.
- leaving supernatant (fluid) at the top
What type of microscope produces a 3D image?
SEM - scanning electron microscope
List key features of TEM
- shoots beams of electrons at a thin sample and detects electrons that make it through to the other side
- very high resolution, so good for learning structure of inner components of a cell
List key features of SEM
- uses focused beams of high-energy electrons which reflect off the surfaces of structures
- produces a 3D image
- samples do not have to be as thin
Describe the key features of a cell surface membrane
1) it is a partially permeable membrane
- controls the exchange of materials between the internal and external cell environment
2) formed from a phospholipid bilayer
Describe the key features of the cell wall
1) offer structural support - provided by cellulose
2) freely permeable
Describe the key functions of chloroplasts
- surrounded by double membrane
- thylakoids - membrane bound flattened discs
- grana joined by flattened membranes - lamellae
Explain the consequence of water loss from vacuoles
1) water moves out of the vacuole and cytoplasm
2) the vacuole shrinks
3) the cell wall is unsupported
4) the plant wilts
What is the main component of fungal cell walls?
- Chitin
List the structures in the nucleus
1) nuclear envelope
2) nuclear pores
3) nucleoplasm
4) chromosomes
5) nucleolus