cell structure Flashcards

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1
Q

basic cell theory

A
  • all living organisms are made of one or more cells
  • the cell is the most basic unit of life
  • all cells arise only from pre existing cells by divison
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2
Q

What common features do cells share?

A
  • every living cell is surrounded by a membrane, which separates the cell contents from everything else outside
  • Dna
  • ribosomes
  • cytoplasm
  • cells contain genetic material which stores all of the instructions needed for the cell’s activities
  • many of these activities are chemical reactions, catalysed by enzymes produced inside the cell
  • cells have their own energy release system that powers all of the cell’s activities
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3
Q

What are the three levels of magnification on a typical high school microscope?

A
  • x40 (low power)
  • x100 (medium power)
  • x400 (high power)
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4
Q

What is the formula to calculate magnification?

A

size of image/actual size of specimen

image size/ actual size = magnification

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5
Q

Explain striated muscle as an atypical example which questions the cell theory

Hello

A
  • building blocks of striated muscle are muscle fibres (similar to cells)
  • they have an average length of 30mm in humans, whereas other cells typically have a size of less than 0.03mm
  • have multiple nuclei
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6
Q

Explain fungi as an atypical example of a cell which questions the cell theory

A
  • fungi consists of narrow thread-like structures called hyphae
  • hyphae have a cell membrane and a cell wall
  • in some types of fungi, the hyphae are divided up into small cell-like sections by cross walls called septa
  • in other types of fungi, however, there are no septa and each hypha is an uninterrupted tube-like structure with many nuclei spread along it
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7
Q

Explain algae as an atypical example of a cell which questions the cell theory

A
  • algae are organisms that feed themselves by photosynthesis and store their genes inside nuclei
  • many algae consist of one microscopic cell
  • giant algae can grow to a length as much as 100mm despite only having one nucleus
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8
Q

Outline the seven functions of life: MR H GREN

A
  1. Nutrition: obtaining food, to provide energy and the materials needed for growth
  2. Metabolism: chemical reactions inside the cell, including cell respiration to release energy
  3. Growth: an irreversible increase in size
  4. Response: the ability to react to changes in the environment
  5. Excretion: getting rid of the waste products of metabolism
  6. Homeostasis: keeping conditions inside the organism within tolerable limits
  7. Reproduction: producing offspring either sexually or asexually

Note: Unicellular organisms must carry out all functions of life in the one cell

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9
Q

Unicellular organisms must carry out all functions of life in the one cell

true of false

A

true

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10
Q

Limitations on cell size

A
  • surface area to volume ratio is important in the limitation of cell size
  • large numbers of chemical reactions take place in the cytoplasm of the cells (metabolism)
  • the rate of the reactions (metabolic rate) is proportional to the volume of the cell
  • substances used for the reactions must be absorbed by the cell and the waste products must be removed; the rate at which substances cross this membrane depends on its surface area
  • surface area:volume ratio is also important for heat production and loss
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11
Q

Outline the ways in which Paramecium demonstrates the functions of life

A
  • Paramecia are surrounded by small hairs called cilia which allow it to move (responsiveness)
  • Paramecia engulf food via a specialised membranous feeding groove called a cytostome (nutrition)
  • Food particles are enclosed within small vacuoles that contain enzymes for digestion (metabolism)
  • Solid wastes are removed via an anal pore, while liquid wastes are pumped out via contractile vacoules (excretion)
  • Essential gases enter (e.g. O2) and exit (e.g. CO2) the cell via diffusion (homeostasis)
  • Paramecia divide asexually (fission) although horizontal gene transfer can occur via conjugation (reproduction)
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12
Q

Outline the ways in which Scenedesmus demonstrates the functions of life

A
  • Scenedesmus exchange gases and other essential materials via diffusion (nutrition / excretion)
  • Chlorophyll pigments allow organic molecules to be produced via photosynthesis (metabolism)
  • Daughter cells form as non-motile autospores via the internal asexual division of the parent cell (reproduction)
  • Scenedesmus may exist as unicells or form colonies for protection (responsiveness)
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13
Q

Emergent properties

A
  • multicellular organisms have properties that emerge from the interaction of their cellular components
  • multicellular organisms can be regarded as cooperative groups
  • the characteristics of the whole organism, including the fact that it is alive, are known as emergent properties
  • emergent properties arise from the interaction of the component parts of a complex structure
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14
Q

Cell differentiation

A
  • specialized tissues can develop by cell differentiation in multicellular organisms
  • different cells perform different functions
  • often a group of cells specialize in the same way to perform the same function (tissue)
  • the development of cells in different ways to carry out specific functions is called differentiation
  • involves the expression of some genes and not others in a cell’s genome (cells have all genes needed to specialize in every possible way but only expresses certain ones)
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15
Q

Stem cell

A
  • can divide again and again to produce copious quantities of new cells; good for the growth of tissues or the replacement of cells that have been lost/damaged
  • not fully differentiated; can differentiate in different ways to produce different cell types
  • essential in embryonic development for the above reasons
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16
Q

What is an example of a non-therapeutic use for embryonic stem cells

A
  • produce large quantities of striated muscle fibres/meat for human consumption
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17
Q

Use of stem cells to treat Stargardt’s disease

A
  • usually caused by a genetic mutation, the membrane protein used for active transport in the retina cells malfunction
  • as a result, photoreceptive cells in the retina degenerate; vision becomes progressively worse
  • can be treated by injecting retina cells derived from embryonic stem cells into the patient’s eyes
  • the cells attach themselves to the retina and improve the vision of the patient
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18
Q

Use of stem cells to treat leukemia

A
  • leukemia is a type of cancer caused by mutations involving the production of abnormally large numbers of white blood cells, which are produced in the bone marrow
  • to cure leukemia, the cancer cells in the bone marrow that are producing excessive white blood cells must be destroyed
  • this can be done via chemotherapy (using chemicals that kill dividing cells) but to remain healthy in the long term, the patient must be able to produce white blood cells needed to fight disease
  • stem cells that can produce blood cells must be present, but they are killed by chemotherapy
  • therefore, they extract stem cells from the patient by sticking a large needle into a large bone (ie. pelvis) and remove the fluid from the bone marrow; they perform chemotherapy, killing the cancer cells but also causing the bone marrow to lose its ability to produce blood cells; the stem cells that were extracted are returned to the patient’s body and they reproduce/re-establish themselves in the bone marrow
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19
Q

Outline the three sources of stem cells and the ethics of using them

A
  1. embryos –> embryonic stem cells
    - almost unlimited growth potential/can differentiate into any type of cell
    - less chance of genetic damage due to accumulation of mutations than with adult stem cells
    - more risk of becoming tumour cells than with adult stem cells
    - likely to be genetically different from an adult patient receiving the tissue
    - ethical controversy because removal of cells from the embyro is likely to kill it
  2. umbilical cord –> cord blood stem cells
    - easily obtained/stored
    - fully compatible with the adult individual that it comes from; no rejection issues
    - limited capacity to differentiate into different cell types (naturally develops into blood cells)
    - limited quantities of stem cells from one baby’s cord
    - ethical because umbilical cord is discarded whether or not stem cells are taken from it.
  3. bone marrow –> adult stem cells
    - difficult to obtain; very few and buried deep in tissues
    - limited capacity to differentiate/less growth potential than embryonic stem cells
    - less chance of malignant tumours developing
    - fully compatible with the individual it comes from; no rejection issues
    - ethical because removal of stem cells does not kill the adult it comes from
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20
Q

Electron microscopes

A
  • much higher resolution than light microscopes
  • reveal the ultrastructure of cells
  • needed to see viruses with diameter of 0.1 micrometres
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21
Q

Resolution

A

Making the separate parts of an object distinguishable by eye

22
Q

Prokaryotes

A

simple cell structure without compartments

  • no nucleus; has nucleoid instead which contains DNA
  • DNA not associated with proteins
  • cell wall
  • do not have crytoplasmic organelles apart from ribosomes
  • ribosomes are 70S (smaller than those in eukaryotoes)
23
Q

How do prokaryotes divide?

A
  • binary fission
  • used for asexual reproduction
  • circular chromosome is replicated and the two copies of the chromosome move to opposite ends of the cell
  • division of the cytoplasm
  • each of the daughter cells contains one copy of the chromosome so they are genetically identical
24
Q

Eukaryotes

A
  • compartmentalized cell structure, meaning that the cell is divided up by single or double membranes into compartments
  • has nucleus
  • DNA associated with histone proteins
  • organelles in the cytoplasm (‘compartments’)
  • ribosomes are 80S (bigger than those in prokaryotes)
25
Q

Advantages to being compartmentalized

A
  • enzymes and substrates for a particular process can be much more concentrated than if they were spread throughout the cytoplasm
  • substances that could cause damage to the cell can be kept inside the membrane of an organelle (ie. lysosome)
  • conditions such as pH can be maintained at an ideal lvel for a particular process, which may be different to the levels needed for other processes in a cell
  • organelles with their contents can be moved around within the cell
26
Q

Recognition features and function of the nucleus

A
  • nuclear membrane is double and has pores through it
  • nucleus contains the chromosomes, consisting of DNA associated with histone proteins
  • uncoiled chromosomes are spread through the nucleus and are called chromatin
  • often densely straining areas of chromatin around the edge of the nucleus
  • DNA replication and transcription to form mRNA occurs in the nucleus (exported via the nuclear pores to the cytoplasm
  • constructs ribosomes
27
Q

Recognition features and function of the rough endoplasmic reticulum

A
  • consists of flattened membrane sacs called cisternae, which have 80S ribosomes attached to them
  • main function is to synthesize protein for secretion from the cell
  • protein synthesized by the ribosomes passes into its cisternae and is then carried by vesicles, which bud off and are moved to the golgi apparatus
28
Q

Recognition features and function of the golgi apparatus

A
  • consists of flattened membrane sacs called cisternae
  • unlike the rER, the cisternae are not as long are often curved, do not have attached ribosomes, and have many vesicles nearby
  • processes proteins brought in vesicles from the rER
  • most of these proteins are then carried in vesicles to the plasma membrane for secretion
29
Q

Recognition features and function of a lysosome

A
  • approximately spherical with a single membrane
  • formed from golgi vesicles
  • contain high [protein] which makes them densely staining in electron micrographs
  • contain digestive enzymes which can be used to break down ingested food in vesicles or break down organelles in the cell or even the whole cell
30
Q

Recognition features and function of a mitochondrion

A
  • surrounded by a double membrane
  • inner membrane invaginated to form structures called cristae
  • the fluid inside is called the matrix
  • the shape is variable but is usually spherical or ovoid
  • produce ATP for the cell by aerobic cell respiration
  • fat is digested here if it is being used as an energy source in the cell
31
Q

Recognition features and function of free ribosomes

A
  • appear as dark granules in the cytoplasm and are not surrounded by a membrane
  • have the same size as ribosomes attached to the rER (80S)
  • synthesize protein, releasing it to work in the cytoplasm, as enzymes or in other ways
  • constructed in a region of the nucleus called the nucleolus
32
Q

Recognition features and function of chloroplast

A
  • surrounded by double membrane
  • variable in shape, but usually spherical or ovoid
  • contains sacs of thylakoids, which are flattened sacs of membrane
  • produces glucose/other organic compounds by photosynthesis
  • starch grains may be present inside the chloroplasts if they have been photosynthesizing rapidly
33
Q

Recognition features and function of vacuoles/vesicles

A
  • consist of a single membrane with fluid inside
  • many plant cells have large vacuoles that occupy more than half of the cell volume
  • some animals absorb foods from outside and digest them inside vacuoles
  • some unicellular organisms use vacuoles to expel excess water
  • vesicles are very small vacuoles used to transport materials inside the cell
34
Q

Recognition features and function of microtubules/centrioles

A
  • microtubules are small cylindrical fibres with multiple roles, including moving chromosomes during cell division
  • animal cells have structures called centrioles, which consists of two groups of nine triple microtubules
  • centrioles form an anchor point for microtubules during cell division and also for microtubules inside cilia and flagella
35
Q

Recognition features and function of cilia/flagella

A
  • whip-like structures projecting from the cell surface
  • contain a ring of nine double microtubules plus two central ones
  • flagella are larger and usually only one is present as a sperm
  • cilia are smaller and many are present
  • both can be used for locomotion
  • cilia can be used to create a current in the fluid next to the cell
36
Q

What are exocrine gland cells of the pancreas and what organelles does it contain?

A
  • a type of gland cell in the pancreas that secretes digestive enzymes into a duct that carries them to the small intestine where they digest food
  • have organelles needed to synthesize/process/secrete proteins (ie. enzymes) in large quantities:
  1. plasma membrane
  2. mitochondrion
  3. nucleus
  4. rER
  5. golgi apparatus
  6. vesicles
  7. lysosomes
37
Q

What are palisade mesophyll cells of the leaf and what organelles does it contain?

A
  • the cell type of the leaf that carries out the most photosynthesis
  • shape is rougly cylindrical
  • contains the following
    1. cell wall
    2. plasma membrane
    3. chloroplast
    4. mitochondrion
    5. vacuole
    6. nucleus
38
Q

Hydrophilic

A
  • substances that are attracted to water
  • all substances that dissolve in water are hydrophilic, including polar molecules such as glucose and chloride ions
  • substances that water adheres to (ie. cellulose) are also hydrophilic
39
Q

Hydrophobic

A
  • substances that are insoluble in water, but dissolve in other solvents
  • molecules that are non-polar are hydrophobic
  • all lipids are hydrophobic
40
Q

What is a phospholipid bilayer?

A
  • a thin polar membrane made of two layers of lipid molecules.
  • the membranes are flat sheets that form a continuous barrier around all cells.
41
Q

Difussion

A

the net movement of molecules from an area where they are at a higher concentration to areas where they are at a lower concentration.
This is due to the random movement of the molecules.
The difference in the concentration of a substance between two areas is called the concentration gradient

42
Q

osmosis

A

the movement of water molecules from a solution with a high concentration of water molecules to a solution with a lower concentration of water molecules, through a cell’s partially permeable membrane.

43
Q

mitosis

A

eukaryotic cell nucleus splits in two, followed by division of the parent cell into two daughter cells.

44
Q

according to cell theory

A

all living organisms are composed of cells

45
Q

spontaneous generation

A

animals such as fleas could arise from dust and that maggots could arise from dead flesh
-believed until 1860
presented by aristotle
disproved by pastuer’s experiments

46
Q

modern cell theory

A
  • activity of an organism deoends on the total activity of independent cells
  • energy flow (metabolism and biochemistry) occurs within cells.
  • cells contain DNA which is specifically in the chromosome and RNA found in the cell nucleus and cytoplasm
  • all cells are basically the same in chemical composition in organisms of similar species
47
Q

exceptions to cell theory

A

-striated muscle: more than one nucleus per cell, up to 300mm- very long,
-aseptate fungal hypae: continuous cytoplasm, many nuclei
-giant algae: large in size (5-100mm) but only one nucleus,
complex structure: bottom rhizoid, long stalk, top umbrella

48
Q

MR H GREN

A
Metabolism
response
homeostasis
growth
excrection
reproduction
nutrition
49
Q

metabolism

A
  • the metabolic pathways e.g. respiration
  • All of the chemical responses that occurs within an organism
  • the web of all the enzyme-catalysed reactions in a cell of organism
50
Q

Why do phospholipids form bilayers in water?

A

their amphipathic nature.
-The polar hydrophilic head group will interact with water to form hydrogen bonds with water as their are attracted to it
but the two hydrophobic tails made of non-polar hydrocarbon tails repel water.

51
Q

emergent properties

A

Multicellular organisms have properties that emerge from the interaction of their cellular components. Emergent properties arise when the interaction of individual component produce new functions
e.g. cell to tissue to organ to human