Cell Injury And Death 2 Flashcards
Apoptosis charcateristics
Programmed cell death
Shrinkage
Regulated intracellular program (activates enzymes that degrade DNA and proteins)
DNA breakdown (cleavage, non random)
Apoptosis steps
Condensation
Fragmentation
Apoptic bodies
Physiological apoptosis
Maintain steady state
Hormone controlled
Embryogenesis - develop hands structure by cell death in webbed section
Pathological apoptosis
Cytotoxic T cell
Virus infected/neoplastic
Damaged with damaged DNA (very pink)
Stages of apoptosis
Initiation - intrinsic/extrinsic pathway
Execution - cleave DNA and compartmentalise
Degradation (phagocytosis)
appear eosinphillic
Intrinsic pathway apoptosis
Signal comes from within cell
Triggers:
- DNA damage
- Withdrawal of growth factors/hormones
P53 protein activated = leaky Mitochondrial membrane
Cytochrome C released - activates CASPASES
Extrinsic pathway apoptosis
Extracellular signals Triggers: - tumour cells, virus infected cells TNFa - tumour necrosis factor alpha - binds to cell membrane (death receptor) Activates CASPASES
What do intrinsic and extrinsic pathways result in
Shrinkage
Formation of apototic bodies
Antigens recognised by phagocytes
Ingested and degraded
Apoptosis vs Necrosis/oncosis
Apoptosis
- single cells
- Shrinkage
- nucleus fragmentation into clumps
- intact plasma membrane & cellular contents
- no inflammation
- eliminate unwanted cells
Oncosis/Necrosis
- groups of cells
- swelling
- pyknosis, karryorrhexis, karyolysis of nucleus
- enzymatic digestion, cell leaks out of cell
- membrane disrupted
- INFLAMMATION
- pathological
Gangrene (wet/gas and dry)
Necrosis visible to naked eye
Wet - infection, liquefactive
Dry - exposure to air, coagulative
Gas - infected with anaerobic bacteria (crash/crush injury, bubbles of bacteria)
Infarction
Necrosis caused by reduction in arterial blood flow
-can result in gangrene
Infarct
Area of necrotic tissue
Loss of arterial blood supply
Causes of infarction and where
Reduction of arterial blood supply
- atherosclerosis
- occlusion (thrombus/thromboembolism)
- twisting (testes/bowel)
- compression
Heart, lungs, brain, kidneys, limbs, GI, testes
Types of infarct
White (usually Coagulative) - solid organs
Red (usually Liquefactive) - cerebral
White infarct
Solid organs - heart, spleen, kidney
Occlusion of end artery
Wedge shaped
Coagulative