cell fractionation Flashcards
1
Q
buffer properties
A
cold and isotonic. why?
2
Q
machine used to blend cell parts
A
homogeniser
3
Q
sample after being homogenised name
A
homogenate
4
Q
how to keep centrifuge stable
A
equal volumes in each sample
5
Q
name of machine used to separate cell carts
A
centrifuge
6
Q
first spin speed for nuclei etc.
A
1000xg for 10 mins
7
Q
name of separated material
A
pellet
8
Q
name of remaining leaf not separated
A
supernatant
9
Q
speed for second spin for mitochondria etc.
A
3500xg 20 mins
10
Q
volume of buffer
A
5cm3
