cell fractionation Flashcards
what must happen before the process can begin
the tissue must be placed in a cold, isotonic buffer solution
why must the solution be cold
to prevent enzyme action
why must the solution be isotonic
to prevent the cell from swelling or bursting
why must the solution be buffer
to maintain a constant pH
what is the first step of cell fractionation
homogenisation - where the cell is broken and so organelles are free
what is the second step of cell fractionation
the organelles must be filtered to remove any large bits of debris
what is the last step of cell fractionation
the organelles are spun (centrifugation) which allows the large organelles to fall to the bottom forming a pellet.
The organelles will be re-spun unit all of them have fallen to the bottom
what is the substance called when the lighter organelles are still floating at the top
supernat
from heaviest to lightest, order the organelles at which they fall at
nucleus
chloroplasts
mitochindria
lysosomes
endoplasmic reticulum
ribosomes
