Cell Division Flashcards
Genome
all the DNA within the cell
Zygote
common ancestor of all human cells
Human somatic (body) cells contain how many chromosomes?
46 chromosomes
-23 copies of paired chromosomes
sex cells only have half the number of chromosomes a body cell would have
Homologous chromosome pairs
are the 2 different copies of the same chromosome in a diploid organism
- one is given by the mother
- one is given by the father
Same size (except X and Y) carries same genes (except X and Y) Genes in same loci (except X and Y) can have different alleles not held together by a centromere
Sister Chromatids
Only exist after DNA replication (100% identical)=Dyads
- same size
- carries same genes
- genes in same loci
- same alleles
- held together by a centromere
Referred as Replicated homologous chromosomes:
-46 replicated homologous chromosomes=23 pairs of homologous chromosomes
Dyads
replicated chromosomes that look like an X
Centromeres
Link sister chromatids together to form a dyad
-also assemble kinetochores
Kinetochores
are proteins that orient themself on either side of a dyad centromere
-attach microtubules to help separate sister chromatids during karyokinesis
Karyokinesis
process where one parent nucleus divides to form two daughter nuclei
Cytokinesis
physical process of cell division
- process that occurs after karyokinesis
- physically separates the cytoplasm and cell membrane
Ploidy
number of chromosome sets four in a cell
Diploid cells
cells that contain two complete sets of chromosomes
Human somatic cells have a diploid number of 46 chromosomes which means each set contains 23 pairs
Haploid cells
have half the number of chromosomal sets as a somatic cell
-uniquely describes gamete cells
Human genets have a haploid number of 23 chromosomes-they contain just one chromosomal set
Gametes
Haploid cells
-contain 23 chromosomes
used by organisms during sexual reproduction
Germ cells
divide by meiosis to form gametes
- diploid
- 46 chromosomes
Cell Cycle
composed of two broad phases
1) Interphase
- 90% of cell cycle, where most cells are found
2) M Phase (Mitosis)
- karyokinesis occurs (division of the replicated DNA and nucleus)
- followed by cytokinesis
Go-Gap Phase 1 (G1) of interphase Sam- Synthesis Phase (S) of interphase Go-Gap Phase 2 (G2) of interphases Make-Mitosis of the M phase Cake- Cytokinesis of the M phase
Gap Phase 1 (G1): of interphase
cell potential grows in preparation of a future division
- cell produces more cytoplasm, proteins, and organelles
- assess conditions if not favorable it will enter G0 phase.
- if conditions are favorable- proceeds to S Phase
Gap Phase 0 (GO) of interphase
cells that will not divide after being made are found here -go about normal function EX: -neuron cells will send action potential -muscle cells will contract NO DIVIDING
Synthesis Phase of Interphase
where a cell will replicate its genome in preparation of an ensuing cell division
-after replication cell enters G2 phase of interphase
Gap Phase 2 (G2) of interphase
cell continues to grow and prepare for division
replicates organelles
checks for:
- accuracy of DNA replication
- Mitosis promoting factor (MPF)
What are the four main phases of Mitosis?
What does a Diploid parent nucleus produce?
2 genetically dental diploid daughter nuclei
Prophase
Metaphase
Anaphase
Telophase
Prophase of Mitosis (M Phase of the cell cycle)
- Chromatin condenses into chromosomes and become more visible
- nucleolus and nuclear envelope begin to disappear
- spindle apparatus beings to form
Metaphase of Mitosis (M phase of the cell cycle)
Spindle apparatus guide chromosomes to the cell equator called the metaphase PLATE
-Dyads lined up in a single fie at the end of meta phase
Anaphase of Mitosis (M phase of the cell cycle)
Shortest step of mitosis
Kinetochore microtubules shorten to pull centromere apart
-sister chromatids now called chromosomes are pulled toward opposite poles
-Polar spindle fibers bigin to elongate the cell
Telophase of Mitosis (M phase of the cell cycle)
Chromosomes arrive at opposite poles and begin to decondense
- nuclear envelope material surround east set of chromosomes
- mitotic spindle breaks down
- spindle fibers continue to push poles apart
Cytokinesis: animal cells
begin in late anaphase by formation of a cleavage furrow
-creates a contractile ring that gets together until it eventually pinches the cell in two
Cytokinesis: Plant cells
begin around telophase
- Golgi apparatus releases vesicle and creating a cell plate
- Cell plate transforms into the middle lamella which cements adjacent plant cells together
What are some mutations that cause a cell to become more cancerous?
mutations in protooncogenes and tumor suppressor genes
Binary Fission
how archaea, bacteria, and mitochondria/chloroplasts (organelles) reproduce
- replicate their genome as cell division is taking place (replication of DNA and division of cell occur at the same time)
- No spindle apparatus
Meiosis
involves same general steps as mitosis
- Meiosis occurs twice (mitosis only occurs once)
- produce a total of 4 haploid daughter cells from one diploid parent
2 parts: Meiosis I-homologous chromosomes are split -reductional division -ploidy of each cell will be halfed Meiosis II-sister chromatids split -looks a lot like mitosis;
Prophase I
chromatin condenses into chromosomes
- nucleolus disappears
- nuclear membrane disassembles
- paring of homologous chromosomes and crossing over occurs which creates genetic diversity
Genetic recombination
occurs when crossover event produces chromosomes that haven’t been seen in either parent
-make genetically diverse offspring
Metaphase I
-bivalents/tetrads line up on metaphase plate duet meiotic spindle apparatus
Anaphase I
separates homologous chromosomes from each other by kinetochore microtubule shortening
- pulled toward opposite poles of the dividing cell
- separates homologous chromosomes; sister chromatids not separated here
Will not start unless one chiasmata Is recognized
Telophase and Cytokinesis I
Telophase I
-meiotic spindle disappears as nuclear envelopes form
Cytokinesis I:
animals-formation of cleavage furrow
plant-formation of cell plate
Prophase II
chromatin condensing into chromosomes
- nucleoli disappearing
- nuclear envolope disintegrating
- meiotic spindle apparatus is formed
Metaphase II
just like metaphase in mitosis
Spindle apparatus guide chromosomes to the cell equator called the metaphase PLATE
- Dyads lined up in a single fie at the end of meta phase
- *each cell will have a haploid number of chromosomes
Anaphase II
pull sister chromatids of each chromosome apart at the centromere
- doubles the number of chromosomes in each cell
- pull chromosomes to opposite poles
Telophase II
reforms a nuclear envelope around each chromosome set
- create two new daughter nuclei
- chromosomes will decondense back into chromatin
- nucleoli will reappear in each nucleu
Cytokinesis II
sepearts each daughter nucleus into two new cells
- produces findal product
- four haploid daughter cells
Chromatin
general packaging of DNA around histone proteins
- helps condense DNA to fit within the nucleus of the celll
- exist in chromosomes during mitosis and meiosis
DNA organization
DNA wraps around proteins called HISTONES forming a complex called nucleosome.
each nucleosome contains 9 histones
Euchromatin
- represents parts of your DNA that consists of “loosely-packed” nucleosomes
- its easier for RNA polymerases to access the DNA code and transcribe our genes
Heterochromatin
represents parts of your DNA that consists of tightly packed nucleosomes
-these areas of DNA tend to be inactive in transcription
DNA helicase
seperates complementary strands at the replication fork
SSB
Single stranded binding proteins
-proteins that prevent two strands from coming together after they seperate
Topoisomerase (DNA gyrase)
relaxes the DNA double helix from the tension that opening the helix created
Primase
Provides a 3’OH group for DNA polymerase to attache nucleotides to
DNA polymerases
class of enzymes that extends DNA in the 5’ to 3’ direction
DNA sliding clamp
Helps hold DNA polymerase to the template strand
DNA ligase
Glues together separate pieces of DNA
Telomerase
Adds repetitive DNA to the ends of eukaryotic chromosomes which prevents critical information from being lost
Operon
a group of of related genes are under the control of one promotor site
Function:
-makes sure the cell conserves its resources unless the resources are required
Lac Operon
HoOperon seen in E. Coli
- ex: inducible operon-usually inactive unless its induced to become active
- induced when glucose is not present but lactose is
Controls 3 genes:
LacZ
LacY
LacA
genes code for proteins that are used in metabolism of lactose
How is the Lac operon controlled
- Lac repressor protein
- encoded by the gene LacL-not apart of lac operon
- constitutively expressed-gene is always being transcribed and translated
- repressor binds to the operator, RNA polymerase will not be able to bind effectively to the DNA and lac genes will be transcribed and translated at low levels
- IN the presence of lactose, allolactose will bind to the lac represser protein, causing a conformational change and cannot bind operator ration - cAMP levels and catabolite activator protein (CAP)
What are the three main types of mutations?
- base substitutions (point mutations)
- Insertion
- Deletion
Point mutations
or called base substitutions
-occur when one nucleotide is replaced by a different nucleotide
3 types:
- Silent
- change in the DNA code result in no change in what amino acid the codon codes for - Missense mutation:
- change in the DNA code results in the codon now coding for a different amino acid
- conservative missense mutation-mutated amino acid has the same properties as the unmated amino acid - Nonsense mutation
- codon is mutated to a stop codon
- protein shorter and can be detrimental to protein function
What happens when a histone protein is acetylated?
Acetylation of histones will remove their positive charge and loosen up the attraction between histones and negatively charged DNA generating euchromatin (Loose DNA)
WHICH INCREASES THE RATE of Transcription
Duplication mutation
piece of DNA is copied then reinserted into DNA
-can cause a frameshift mutation
Translocation mutation
when one sequence of nucleotides is excised and reinserted into the DNA sequence somewhere else
-can cause a frameshift mutation
Inversion mutation
type of mutation where a sequence of DNA is excised and flipped and reinserted back into the DNA
Mismatch Repair
DNA polymerase can’t catch all its mistakes
-cell marks the uncaught errors so they can be replaced with the correct sequence
Nucleotide excision repair
repair mechanism for DNA that has been damage.
-damaged strand is cut out and replaced with the correct sequence based on complementary base pairing
2 life cycles of viruses
Lysogenic cycle
- viral nucleic acid will insert itself into the host’s genome
- beneficial to virus because its nucleic acid will be replicated whenever the hosts DNA is replicated
- virus is dormant and does not harm the host
Lytic cycle
- virus takes over the host’s cells’s machinery and does en up harming the host
- breaking apart host genome and replicating many copied of viral nucleic acid; make viral proteins which can lyse (break apart) the host cell and go infect other cells
HIV
human immunodeficiency virus
- RNA virus/retrovirus-a virus that stores its genetic material as RNA
- carry an enzyme called reverse transcriptase which allows them to convert their RNA into complement DNA (cDNA)
wild type allele
normal allele that is most common in nature
Hemizygous
individuals will only have one copy of an allele instead of two
penetrance
the proportion of individuals who exhibit the phenotype of an allele for a given gene
Complete penetrance
vs
Incomplete penetrance
the trait is expressed in the full population that has the allele
when the trait is only expressed in only part of the popuatonin that has the allele
Expressivity
degree of a certain phenotype
Incomplete dominance inheritance
an intermediate between two phenotypes
Ex: white with red flow producing pink flower