Cell culture (lab 3)

Question 1

Merisenatic tissue

Answer 1

cells capable of cell division or elongation

Question 2

apical meristem

Answer 2

growth of a plant at the tips of the plant

Question 3

lateral meristem

Answer 3

growth of a plant alongside the stem or root

Question 4

terminal bud

Answer 4

contains a shoot apical meristem

Question 5

auxiliary bud

Answer 5

contains a shoot lateral meristem

Question 6

lenticel

Answer 6

a small corky, oval or elongated area on the surface of a plant stem, trunk, or fruit that allows the interchange of gases

Question 7

totipotency

Answer 7

cells differentiated into other organs or tissues depending on the place of the cells and the moment

Question 8

plasticity

Answer 8

concept of modifying the plant’s shape depending on the environment

Question 9

phytohormone

Answer 9

crucial for the making of plant tissues

Question 10

somatic embryogenesis

Answer 10

new embryo formed from somatic cells

Question 11

somatic cell

Answer 11

any cell not involved in the reproduction system

Question 12

explant

Answer 12

part of a plant tissue used for starting a culture

Question 13

medium

Answer 13

liquid or solid containing glucose (and other) to grow the explants
~> since they are not doing any photosynthesis they need sugar

Question 14

flow cabinet

Answer 14

all work is done in a sterilized environment to prevent contamination

Question 15

hemocytometer

Answer 15

microscope slide to count the number of cells with 9 squares

Question 16

trypan blue

Answer 16

blue colorant used in order to see dead and alive cells

Question 17

% of viable cells

Answer 17

(#viable cells) / (total cells) X100

Question 18

Average

Answer 18

(#Viable cells) / (#Squares)

Question 19

Dilution

Answer 19

(Cell Solution Vol) / (Colorant Solution Vol)

Question 20

Cell density

Answer 20

Average x dilution x 10⁴ (cell/mL)

Question 21

Seed culture

Answer 21

culture of seed to raise sterile seedling through in vitro

Question 22

Embryo culture

Answer 22

isolation of an embryo given from an organism for in vitro growth

Question 23

Callus

Answer 23

unspecialized, unorganized and a dividing mass of cells

Question 24

Callus culture

Answer 24

the growth of a callus which involves into organ differentiation

Question 25

Cell suspension culture

Answer 25

culture in a suspended and liquid medium