Cell Blocking

Question 1

involves the preparation of cells by histopathologic procedure

Answer 1

Cell Blocking

Question 2

used for diagnosis when cancer is suspected or to provide evidence
of metastatic disease

Answer 2

Cell Blocking

Question 3

performed in conjunction with Papanicolaou smear preparation

Answer 3

Cell Blocking

Question 4

Materials suitable for the Cell Block procedure

Answer 4

Sputum containing cells in thick mucus.
Effusions in which cells are growing as a state of cell culture.
Washings containing cell clusters, which are mechanically exfoliated.

Question 5

What anticoagulant are used to collect fluids?

Answer 5

Potassium Oxalate

Heparin or Sodium Citrate - Citric Acid

Question 6

Volume of coagulant for each 100 mL of fluid

Answer 6

2 mL

Question 7

Should be processed in the fresh state promptly

Answer 7

Samples

Question 8

Describing the general characteristics of the sample and volume received

Answer 8

Gross Examination

Question 9

Descriptive terms used in gross examination

Answer 9

Blood-tinged
Clear
Yellow
Amber
Frothy
Mucinous

Question 10

General Processing of Samples

Answer 10

1. Agitate the fluid slightly and pour into test tubes.
2. Centrifuge at medium speed for 15 to 30 min.
3. Pour off supernatant.
4. Prepare two smears for the Papanicolaou technique from the sediment.
5. Pour a small amount of fixative directly into the test tube containing the sediment to
   coagulate it and let it stand for 15 to 30 minutes.
6. Wrap the recovered sediment using lens paper or gauze.
7. Immerse in the fixative for 6 to 24 hours depending on the size of the coagulated
   sample.
8. Perform routine procedure for dehydration, clearing, infiltration, embedding,
   sectioning and staining.