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Histopathology: Cell Blocking > Automated and Rapid Tissue Processing > Flashcards

Automated and Rapid Tissue Processing Flashcards

1
Q

makes use of an automatic tissue processing machine that fixes,
dehydrates, clears and infiltrates tissue

A

AUTOMATED TISSUE PROCESSING

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2
Q 
decreases time (approximately 16 hours) and labor needed during tissue
processing → more rapid diagnosis with less technicality
A

AUTOMATED TISSUE PROCESSING

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3
Q

COMPONENTS OF TISSUE PROCESSORS

A
  1. ELECTRICAL TIME CLOCK
  2. CIRCULAR SUPERSTRUCTURE
  3. TRANSFER ARM
  4. RECEPTACLE BASKET
  5. CIRCULAR DECK
  6. BEAKERS
  7. WAX BATH
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4
Q

controls the time intervals in the immersion and transfer of

tissue samples from one reagent to another

A

ELECTRICAL TIME CLOCK

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5
Q

contains the basket carrier, receptacle basket and receptacles

A

CIRCULAR SUPERSTRUCTURE

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6
Q

moves the tissues from one processing reagent to another

A

TRANSFER ARM

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7
Q

holds the specimen

A

RECEPTACLE BASKET

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8
Q

holds the reagent beakers and paraffin wax baths

A

CIRCULAR DECK

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9
Q

contain the reagents

A

BEAKERS

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10
Q

contains the paraffin

A

WAX BATH

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11
Q

CARE OF THE TISSUE PROCESSOR

A

✓ Change reagent solutions depending on the number and sizes of tissue samples being
processed in the laboratory
✓ Presence of odor, contamination or cloudiness in a reagent solution indicates that
changing is required
✓ Wax baths should be filled to the appropriate level
✓ Accumulated wax in beakers or on any surface of the machine must be removed and
any spillage should be wiped
✓ Receptacles and baskets are cleaned of residual paraffin by immersion in xylene
✓ Clean all nylon parts with detergents (do not use acid)
✓ wax bath thermostats should be set at least 3oC above the melting point of the wax
✓ check the timing when loading the machine

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12
Q

ADDED WHEN THERE IS A PRESENCE OF ODOR OF CLEARING AGENT

A

PARAFFIN

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13
Q

HOW MANY BEAKERS CONTAIN THE SAME SOLUTION

A

3-4

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14
Q

WHICH BEAKER REQUIRES A FRESH CHANGE

A

THE LAST

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15
Q

PERCENTAGE OF DEHYDRATING AGENT

A

100%

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16
Q

WHEN IS DEHYDRATING AGENT CHANGED

A

AFTER 2 COMPLETE LOADS

17
Q

WHEN IS LOWER DEHYDRATING AND CLEARING AGENTS CHANGED

A

AT LEAST ONCE A WEEK

18
Q

MACHINES FOR RAPID TISSUE PROCESSING

A
  1. VACUUM TISSUE PROCESSOR
  2. MICROWAVE OVENS
  3. CONTINUOUS INPUT RAPID TISSUE PROCESSOR
19
Q

tissues are placed inside a retort chamber

A

VACUUM TISSUE PROCESSOR

Histopathology: Cell Blocking (4 decks)

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