Cell Biology Flashcards
Replicative senescence
decline in cell replacement and tissue repair
What characterizes senescent cells?
size expansion, prominent Golgi, enlarged nuclei, vacuolated cytoplasm, enlargement of lysosomes, resistance to apoptosis
Factors causing senescence
epigenetic alteration, mitochondrial dysfunction, telomere attrition, unfolded protein response, DDR, aneuploidy, acquisition of secretory phenotype in non-dividing cells, loss of proliferation
What are some interventions to prevent senescence?
restoration of telomeres, removal of senescent cells, caloric restriction
What is trypan blue used for?
it is a dye that dyed the nonviable cells. This dye could not get through the membrane of the viable cells
What was the purpose of adding trypsin in the cell bio lab?
Trypsin would allow for the cells to detach
What was used to maintain a sterile environment in the cell bio lab?
70% EtOH
Which tissues have least potential for cell replacement?
nerve and muscle
Which tissues have greatest potential for cell replacement?
connective and epithelial
Senescence
stage at which cells are alive, but stop dividing and cannot respond to normal growth factors that induce cell division
In context with the cell biology unit, what does immortalized mean?
It means the cells will continue to divide and never enter senescence
How do you calculate dilution factor?
total volume/aliquot volume
How do you calculate cell viability?
viable cells/(viable cells + nonviable cells) * 100
