Cell Biology Flashcards
What is cell biology also known as?
Cytology
A cell is the most basic working unit of _______
life
New cells only arise how?
From existing cells, by cell division
What are the two basic types of microscopy?
Light microscopy, electron microscopy
What type of microscopy is dependent on on transmitted light to illuminate the biological sample of interest?
Light microscopy
What type of light microscopy is the conventional form of light microscopy, using visible light as the source?
Brightfield microscopy
Which type of microscopy depends on ultraviolet light to illuminate the specimen against a black background?
Fluorescence microscopy
What type of microscopy depends on an electron beam to bombard the specimen (which has normally been killed by chemical fixation prior to examination)?
Electron microscopy
What is the key advantage of electron microscopy over light microscopy?
A greater resolution (by at least 100x)
When an electron beam passes through an ultrathin, carefully prepared section of the specimen to reveal the contents of the cell which is “stained” with heavy metal it is __________
Transmission Electron Microscopy (TEM)
When a specimen remains intact, but gets coated with a thin film of heavy metal so that the beam is deflected off the contoured surface to yield a 3-d image it’s called __________
Scanning Electron Microscopy (SEM)
What separates the cytoplasm of the cell from the cell exterior?
the cell (plasma) membrane
What accumulates as a protective matrix along the membrane’s outer surface?
Biomolecules
The cytoskeleton in made of microfilaments of a protein called _____
Actin
Complexity, ex. possession of more types of organelles, is more common in _____ ____
eukaryotic cells
Which cells have no membrane bound nucleus?
Prokaryotic
Which cells are small and unicellular or occasionally colonial? (NEVER multicellular)
Prokaryotic cells
What is composed of a phospholipid bilayer with other components like protein channels that governs entry and exit of materials in the cell?
The cell membrane
The cell membrane represents the major, and usually only, membrane system of a ________ cell.
Prokaryotic
What does the cytoplasm of a prokaryotic cell contain?
a nucleoid region (DNA), and ribosomes
Prokaryotic cells rarely possess a vacuole lined by a membrane called ______
the tonoplast
Tonoplasts help to retain ___ _______
cell turgor
Prokaryotes also have external features like a cell wall, glycolayx, pili, and ________
flagella
The cell wall protects the cell membrane and _______
cytoplasm
What is secreted externally to the cell wall as a slime layer or capsule?
The glycocalyx
The glycocalyx enables the cell to adhere to substrates or to eachother (i.e. to form a ________)
colony
Short abundant extensions that assist in attachment to surfaces and even other cells are called ______
Pili (pilus singular)
What part of the cell is used for locomotion and possess proteins called microtubules which permit a flexible whip-like action?
Flagella (singular flagellum)
Which cells can be singular, colonial, or commonly multi-cellular?
Eukaryotic cells
Eukarya contains what four groups?
Fungi, Animalia, Plantae, Protista (often unicellular)
Eukaryotes all have what?
A membrane bound nucleus