cell

Question 1

The most common form of spontaneous replication error is

Answer 1

tautomeric shift

Question 2

DNA replication is

Answer 2

semiconservative

Question 3

Strand slippage results in _____, which can cause a number of human diseases such as Fragile X Syndrome

Answer 3

the re-replication of highly repetitive DNA sequences

Question 4

Which of the following disease(s) is characterized by a Trinucleotide Repeat Disorder

Answer 4

All of the above

Question 5

Nonhomologous end joining is used to

Answer 5

fix double-strand DNA breaks

Question 6

Which of the following DNA repair mechanisms is most prone to error?

Answer 6

SOS repair

Question 7

What type of DNA damage is induced by UV radiation?

Answer 7

pyrimidine dimer formation

Question 8

Base analogues, base-modifying agents and intercalating agents are examples of

Answer 8

Chemical mutagens

Question 9

Is nonhomologous or homologous end joining more accurate?

Answer 9

Homologous end joining

Question 10

Multiple sites of DNA replication along eukaryotic chromosomes are known as

Answer 10

replicons

Question 11

The protein shugoshin is crucial for meiosis I, because it

Answer 11

protects centromere cohesins from degradation by separase

Question 12

A black Labrador retriever named Angus is heterozygous (Bb) for coat color (B = black andb = brown). Bb is Angus’..

Answer 12

genotype

Question 13

Meiosis I results in the formation of

Answer 13

2 diploid cells

Question 14

The synaptonemal complex becomes fully developed and crossing over occurs in which stage of Prophase I.

Answer 14

pachytene

Question 15

New combinations of genetic material give rise to generic variability in sexual reproducing organisms through…

Answer 15

All of the above

Question 16

Oocytes undergo their first arrest in what stage of meiosis?

Answer 16

Prophase I

Question 17

Resumption of Meiosis is oocytes requires an increase in MPF activity which is triggered by ______

Answer 17

Progesterone

Question 18

Recombination in bacteria can occur via

Answer 18

All of the above

Question 19

A bacterial cell in which the F plasmid is integrated into the chromosome is known as

Answer 19

an Hfr cell

Question 20

During homologous recombination ____ in eukaryotes coats the single stranded DNA and helps in homology searching

Answer 20

RAD51

Question 21

The observed “puffs” in the polytene chromosomes of Drosophila are sites of

Answer 21

transcription

Question 22

_______ of the DNA can directly block the binding of transcription factors and promotes chromatin packing leading to the formation of heterochromatin

Answer 22

Methylation

Question 23

Which of the following is an example of translational regulation?

Answer 23

mRNA degradation

Question 24

Which of the following is NOT a structural motif associated with transcriptional regulation?

Answer 24

CpG motif

Question 25

A male is tested for cellular abnormalities. The technician notes that there are 2 Barr bodies present within each of the patient’s cells. This indicates that his sex chromosomes are most likely

Answer 25

XXXY

Question 26

A DNA element that decreases transcirption of a gene from a vast distance is known as a(n)…

Answer 26

silencer

Question 27

Genes associated with embryonic development are known as

Answer 27

homeotic (HOX) genes

Question 28

Eukaryotic gene expression is regulated at 5 main levels

Eukaryotic gene expression is regulated at several levels, including:

Answer 28

the genome
transcription
post-transcription
translation
post-translation

Question 29

_______ are DNA sequences that are sometimes employed to prevent an enhancer or silencer from affecting the wrong genes

Answer 29

Insulators

Question 30

Ubiquitin targets proteins form degradation by proteasomes.

Answer 30

True

Question 31

Analyzing the expression of thousands of genes simultaneously can be accomplished using which of the following techniques

Answer 31

DNA microarrays and RNA sequencing

Question 32

This blotting technique is used to quantify the amount of mRNA by subjecting the mRNA to electrophoresis and transferring it to a special blotting paper.

Answer 32

Northern Blotting

Question 33

A cDNA library

Answer 33

doesn’t contain introns because it is generated by copying messenger RNA with the enzyme reverse transcriptase and cloning the resulting complementary DNA

Question 34

Whole genome or shotgun sequences requires

Answer 34

the creation of overlapping DNA fragments whose sequence can be aligned into large contiguous fragments (contigs) using computer algorithms

Question 35

Which column chromatography would be best utilized when looking for a specific protein of interest?

Answer 35

affinify

Question 36

Gel electrophoresis separates DNA molecules based on variability in their

Answer 36

size

Question 37

Bacterial and yeast artificial chromosomes are useful tools in molecular biology because

Answer 37

they can carry large DNA fragments such as large eukaryotic genes

Question 38

Next generation sequencing methods are revolutionizing biology research and human medicine because they

Answer 38

All of the above

Question 39

In combination with homologues recombination, the CRISPR/Cas system can be used to accomplish all of the following EXCEPT

Answer 39

DNA cloning

Question 40

Only 0.3% of nucleotide bases will vary from person to person, creating features that make us unique as individuals

Answer 40

True