CCEA Specification - GCSE Biology Flashcards
Explain how greater resolution of electron microscopes has increased our understanding of cell structures.
(9 marks)
- TEM’s pass beams of electrons through a specimen to give us a clear image.
- SEM’s scan over a specimen with a low energy electron beam stimulating emission of high-energy backscattered electrons + low-energy secondary electrons from the surface of the specimen.
- EM’s resolution can show details that are 0.0001µm apart; compared to LM’s which shows details that are 0.2µm apart.
- This feature of the EM allows us to see clearly inside the Cell/Cell’s organelles including chloroplasts/mitochondrion; A LM needs a magnification of roughly x1500 so that our eyes can see larger cell structures in low resolution.
- EM’s also have a high magnification rate which along with the high resolution can provide us with an inside look into the function of a Cell.
- It means we can generate a connection between different Cells/organelles in the body and better understand how the body is run.
List the parts of a Light Microscope.
5 marks
- Eyepiece
- Objective Lens
- Stage
- Focusing Knob
- Light Source
Describe TEM’s
2 marks
Type of EM’s.
- Pass beams of electrons through a specimen to give us a clear image.
Describe SEM’s.
4 marks
Type of EM’s.
Scan over a specimen with a low energy electron beam stimulating emission of;
- high-energy backscattered electrons
- and low-energy secondary electrons from the surface of the specimen.
Compare the resolution of Electron Miscroscopes and Light Microscopes.
(6 marks)
- EM’s resolution can show details that are 0.0001µm apart;
LM’s which shows details that are 0.2µm apart. - This feature of the EM allows us to see clearly inside the Cell/Cell’s organelles including chloroplasts/mitochondrion.
- LM needs a magnification of roughly x1500 so that our eyes can see larger cell structures in low resolution.
Describe; Electron Microscopes vs Light Microscopes magnification Rate.
(6 marks)
- EM’s have a high magnification rate which along with the high resolution can provide us with an inside look into the function of a Cell.
- LM’s have a low magnification rate meaning we can see no further than large organelles in Cells at low resolution.
How can we convert Metres (m) into Millimetres (mm)?
1 mark
m to mm = x 1000.
How can we convert Millimetres (mm) to Metres (m)?
1 mark
mm to m = divide by 1000
How can we convert Millimetres (mm) to Micrometres (µm)
1 mark
mm to µm = x 1000
How can we convert Micrometres (μm) to Millimetres (mm)?
1 mark
mm to μm = divide by 1000
How can we convert Micrometres (μm) to Metres (m)?
1 mark
μm to m = divide by 10,000,00
How can we convert Metres (m) to Micrometres (μm)?
1 mark
m to μm = x 10,000,00
What are the Sl prefixes (to the power of 10) to convert numbers from Metres to other Sl units?
(8 marks)
Milli (mm)- x 10^-3
Micro (μm)- x 10^-6
Nano (nm)- x 10^-9
Pico (pm)- x 10^-12
Place Sl units in order from largest to smallest.
5 marks
Metre (m) Millimetre (mm) Micro (μm) Nano (nm) Pico (pm)
How can we convert Metres (m) to Kilometres (km)?
1 mark
m to km = divide by 1000
How can we convert Centimetres (cm) to Millimetres (mm)?
1 mark
cm to mm = x 10
How can we convert Metres (m) to Centimetres (cm)?
1 mark
m to cm = x 100
How can we convert Kilometres (km) to Metres (m)?
1 mark
Km to m = x 1000
Place Sl base units in order from largest to smallest.
4 marks
Kilometres (km)
Metres (m)
Centimetres (cm)
Millimetres (m)
What is the equation for magnification?
2 marks
Magnification = size of image / size of real object.
How do we find the actual length of an object from a magnified image?
(2 marks)
Actual length = image size / magnification
How can we find the total magnification from a microscope?
2 marks
Total Magnification = Eyepiece Magnification x Objective Lens Magnification
How can we find the magnification size from an image?
2 marks
Magnification = Length of Image / Actual Length
How can we convert Millimetres (mm) to Centimetres (cm)?
1 mark
mm to cm = divide by 10
List the parts of an animal cell.
19 marks
Main Organelles: Nucleus, Chromosomes (containing Chromatin), Cytoplasm, Mitochondria, Cell Membrane, Plasma Membrane.
Others: Nucleolus, Nuclear Membrane, Ribosomes, SEM, REM, Vacuole, Golgi apparatus, Golgi Vesicle, Cytoskeleton, Lysosomes, Secretory vesicles + Peroxisome
Give a brief description of the Nucleus and it’s role in the Cell.
(6 marks)
Contains the cell’s genetic information arranged in chromosomes.
Control centre of the cell - activities controlled include; growth, division, and protein synthesis.
Give a brief description of the Chromosomes and it’s role in the Cell.
(7 marks)
Threadlike structure found in the nucleus. Made of DNA which contains the genetic information of the cell - Chromatin holds this DNA (and protein) here.
To fit in the Nucleus DNA molecules are wound around histones to form chromosomes.
Give a brief description of the Cytoplasm (Cytosol) and it’s role in the Cell.
(3 marks)
Gelatinous liquid in the Cell in which organelles are suspended - composed of water, salts + various organic molecules.
Chemical reactions take place here.
Give a brief description of the Mitochondria and it’s role in the Cell.
(4 marks)
- Large organelles that have both an inner/outer membrane, as well as their own mitochondrial DNA.
- It is the site of Cellular Respiration in cells, where O2 + glucose are converted into ATP which cells use for energy.
Give a brief description of the Cell Membrane and it’s role in the Cell.
(7 marks)
- Selectively permeable barrier, composed of a Phospholipid Bilayer, surrounding the cell.
- Controls what substances enter + leave the Cell while holding the animal Cell together.
- Embedded in the Membrane are macromolecules (e.g. glycoproteins) and Protein Channels.
Give a brief description of the Nuclear Membrane and it’s role in the Cell.
(4 marks)
- Porous Membrane that allows RNA + proteins to pass in and out of the nucleus.
- Eukaryotic cells contain a “true” nucleus, NM is the structure that defines the boundaries of the Nucleus.
Describe the structure of the Nucleus.
6 marks
Made up of Nuclear lamina, Nucleolus, Chromatin and Nuclear Envelope; (inner/outer membrane) perforated with Nuclear Pores.
Describe the structure of the X Chromosome.
8 marks
Two sex determining chromosomes (allosomes) found in both Male/Female organisms;
- Contains 2 sister Chromatids, Centromere, Telomeres, DNA molecule, p (short)arm and q (long) arm.
Describe the structure of Cytoplasm.
4 marks
Jelly-like substance; contains water, salts, organic compounds and organelles found in the cell.
Describe the structure of the Plasma Membrane (Fluid Mosaic Model).
(20 marks)
Phospholipid Bilayer - Each phospholipid is amphipathic. Made of;
- 2 hydrophobic fatty acid tails facing inwards towards one another.
- Phosphate-linked hydrophilic head faces outward allowing substances to easily pass (e.g. O2, CO2).
- Glycerol.
Cholesterol, (another lipid) composed of;
- 4 fused carbon rings (alongside the phospholipids) in the core of the membrane.
Proteins;
- May extend partway into the plasma membrane,
- Cross the membrane entirely (protein channels)
- or be loosely attached to its inside or outside face.
Carbohydrate groups;
- only on the outer surface.
- attached to proteins + lipids: forming glycoproteins/ glycolipids.
Cytoskeleton;
- Works closely with the membrane to maintain the Cells shape