CC section

Question 1

Concentration of the substance is directly proportional to the light absorbed and inversely proportional to the logarithm of the light transmitted by the soln

Answer 1

Beer Lambert’s Law

Question 2

principle of Glucose

Answer 2

hexokinase

Question 3

principle of creatinine

Answer 3

kinetic alkaline picrate

Question 4

principle of uric acid

Answer 4

uricase

Question 5

principle of urea nitrogen

Answer 5

urease

Question 6

principle of bilirubin

Answer 6

diazo rxn

Question 7

principle of albumin

Answer 7

bromocresol green

Question 8

principle of chole

Answer 8

cholesterol oxidase

Question 9

principles of Abbott Architect and its test application

Answer 9

Photometric (Beer Lamberts Law): blood chem
Potentiometry: Na, K, Cl
CMIA: Troponin I, CKMB, and other sero tests

Question 10

Used to measure electrolytes using ion- specific electrodes

Answer 10

potentiometry

Question 11

used for Na electrode

Answer 11

Crown ether ionophore

Question 12

electrolye affected by LIPEMIC samples

Answer 12

Na

Question 13

used for K electrode

Answer 13

Valinomycin

Question 14

used for Cl electrode

Answer 14

Silver chloride

Question 15

Chemical rxn that emits energy in the form of light. When used in combination with IA technology, the light produced by the rxn indicates the amt. of analyte in a sample

Answer 15

CHEMILUMINESCENT MICROPARTICLE IMMUNOASSAY (CMIA)

Question 16

what type of assay is CHEMILUMINESCENT MICROPARTICLE IMMUNOASSAY (CMIA)

Answer 16

Non-competitive sandwich assay

Question 17

T or F

in CMIA,

Amt. of signal = Amt. of analytes present

Answer 17

T

Question 18

molecule that react as a part of
assay, so a change in signal can be measured in urine after + reagent soln.

Answer 18

label

Question 19

electrolytes analyzed by SmartLyte Electrolyte Analyzer

Answer 19

Na
K
Cl
Ca++ (iCa)

Question 20

principle of SmartLyte Electrolyte Analyzer

Answer 20

Direct ISE

Question 21

Analytical technique used to determine ions activity in aqueous soln by measuring electrical potential. Relatively inexpensive; easy to operate

Answer 21

Direct ISE

Question 22

RV of Na

Answer 22

136 - 145 mmol/L

Question 23

RV of K

Answer 23

3.5 - 5.1 mmol/L

Question 24

RV of Cl

Answer 24

97 - 111 mmol/L

Question 25

RV of Ca++ or iCa

Answer 25

1 - 1.3 mmol/L

Question 26

analyte performed in TOSOH G11

Answer 26

HbA1c

Question 27

gold std for HbA1c

Answer 27

HPLC

Question 28

PP of HbA1c

Answer 28

0

Question 29

retention time of HbA1c

Answer 29

0.47 +/- 0.01

Question 30

total area of HbA1c

Answer 30

600 - 3000

Question 31

Give the Criteria for rejection

Answer 31

• Improper test requests (incomplete, duplicate, errors in test input, inconsistent info) • Inappropriate transport • Spx without or unsuitable barcodes • Misidentification • Improper container or tube (includes CSF) • Insufficient spx volume • Incorrect preservation, storage • Lipemic • Hemolyzed • Clotted samples with fibrin

Question 32

commonly used confidence interval

Answer 32

95% fall within +/- 2 SD of the mean

Question 33

NEQAS

Answer 33

NATIONAL EXTERNAL QUALITY ASSESSMENT SCHEME