Carriage of O2 and CO2 in the Blood Flashcards
What are the 2 main ways in which oxygen is carried in the blood?
Dissolved in the blood or combined with haemoglobin
What equation is used to determine the amount of oxygen that dissolves in the blood?
volume of oxygen = 0.0232 x pO2
0.29 = 0.0232 x 12.5
By the time the oxygen reaches the alveoli, the pO2 is 12.5 kPa
What equation is used to determine partial pressure?
partial pressure = fractional concentration x barometric pressure
What is barometric pressure in the atmosphere?
101.3 kPa
How much oxygen is dissolved in one decilitre of blood?
0.29 ml of oxygen is dissolved in each 100 ml of blood
In cold water, how does the amount of gas dissolved differ than at 37 degrees?
There is more oxygen and CO2 dissolved in cold water
More gas will dissolve at lower temperatures
What is the significance of the value 0.0232 in the equation?
Is it a constant?
It describes the solubility of oxygen
Calculations are always at 37oC so it does not change
The value changes if the calculations are worked out at a different temperature
What is oxygen saturation?
It describes how much of the haemoglobin in the blood is bound with oxygen, compared to how much haemoglobin there is in total
How is oxygen saturation calculated?
Amount of oxyhaemoglobin divided by the total amount of haemoglobin
HbO2 / (HHb + HbO2)
What do SO2 HbO2 HHb stand for?
SO2 - oxygen saturation
HbO2 - oxyhaemoglobin
HHb - deoxyhaemoglobin
What equation is used to determine how much oxygen is being carried by haemoglobin?
volume of O2 = SO2 x [Hb] x 1.39
19 = 0.97 x 14 x 1.39
What is the Hüfner constant and what does it describe?
1.39
it describes the number of ml of oxygen that each gram of Hb can carry
What is normal haemoglobin concentration?
14 grams per 100 ml
How much oxygen is carried by Hb per 100 ml of blood?
Hb can carry 19 ml of oxygen per 100 ml of blood
How many amino acids are in each of the 4 polypeptide chains that make up Hb?
141 - 146 amino acids per chain
What is the tertiary structure of Hb?
A globular structure determined by the order of amino acids in the primary structure
It forms a crevice in which the haem group will sit
What are the 2 forms in which Hb exists?
What does this describe?
Relaxed and tense forms
This describes how tightly packed the globin is within the chain
What does Hb act/look like in the relaxed state?
The globin is more spread out
The crevices containing the haem group becomes slightly wider
The oxygen can access the haem group more easily
What does Hb act/look like in the tense state?
The globin is more tightly condensed
The crevices become too small for the oxygen to fit into easily
What happens upon binding of the first oxygen molecule to Hb?
As soon as one molecule of O2 binds to the haem group, the shape of the globin chain changes
This alters how tense the adjacent globin chains are
The globin chains relax a bit more with each O2 that binds
Why does pO2 of the blood have to be high before Hb begins to be oxygenated?
The first molecule of oxygen does not bind to the Hb easily
When deoxygenated blood returns to the heart, how saturated is the Hb and why?
Hb is still 75% oxygenated
As blood circulates around the body, only 25% of O2 that is bound to Hb is actually consumed
Under normal circumstances, what part of the Hb molecule is used to carry oxygen?
Only the final binding site on each Hb molecule
This is the site that works the most efficiently
What happens as partial pressure of oxygen becomes lower?
It becomes harder to carry oxygen in the blood
What is meant by the term ‘cooperativity’?
The way in which altering the state of one part of the protein affects the other components
What causes the shape of the oxygen dissociation curve?
The cooperativity between polypeptide chains in the Hb molecule
What happens at the bottom of the oxygen dissociation curve?
It is difficult to get the oxygen to bind to Hb
The partial pressure must be increased significantly before oxygen starts to bind
What happens at the top of the oxygen dissociation curve?
All of the oxygen binding sites are occupied and the curve levels off
What does the arterial point on the oxygen dissociation curve show?
The saturation of normal arterial blood
Saturation is around 97% and partial pressure of oxygen is 12.5 - 13 kPa