carbs and lipids Flashcards

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1
Q

What is a monomer?

A

. A monomer is a smaller / repeating) unit / molecule from which larger molecules / polymers are made

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2
Q

A starch molecule has a spiral shape. Explain why this shape is important to its function in cells.

A

. Compact/occupies small space/tightly packed;

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3
Q

The structure of cellulose is related to its role in plant cell walls. Explain how. (3)

A

. Long, straight, unbranched chains of Beta glucose;
. (Joined by) many WEAK hydrogen bonds;
. Form microfibrils / macrofibrils;
. Provide rigidity/strength/support;

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4
Q

Give one feature of starch and explain how this feature enables it to act as a storage
substance.

A

. Helical / spiral So compact / tightly packed / can fit (lots) into a small space;
. Insoluble So no osmotic effect / does not leave cell / does not affect water potential;
. Large molecule / long chain (So) does not leave cell / contains large number of glucose units;
. Branched chains (So) rapid hydrolysis to remove glucose for respiration;

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5
Q

Hydrogen bonds are important in cellulose molecules. Explain why.

A

. Holds chains/cellulose molecules together/forms cross links between chains/cellulose molecules/forms microfibrils;
. Providing strength/rigidity (to cellulose/cell wall);
. Weak Hydrogen bonds provide strength in large numbers;

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6
Q

Describe how lactose is formed and where in the cell it would be attached to a polypeptide to form a glycoprotein

A

. Glucose and galactose
. Joined by condensation
. Joined by glycosidic bond
. Added to polypeptide in Golgi

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7
Q

Describe how the student would show that reducing sugars were present in a solution.

A

. Add Benedict’s;
. Heat to 95°C;
. Red/orange/yellow/green precipitate (shows reducing sugar present);

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8
Q

Describe how you would test a sample of food for the presence of starch.

A

. Add potassium iodide (KI) solution to the food sample;
. Blue/black/purple indicates starch is present;

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9
Q

Describe how you would test a liquid sample for the presence of lipid box and how you would recognise a positive result.

A

. (Mix / shake sample) with ethanol, then water and shake;
. Cloudy White / milky (emulsion);

. Add ethanol and shake
. Then add water and shake again
. Cloudy white emulsion forms if lipid present

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10
Q

Omega-3 fatty acids are unsaturated. What is an unsaturated fatty acid?

A

. Double bond(s);
. (Bonds) between carbon atoms within the hydrocarbon chain;

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11
Q

Describe how an ester bond is formed in a phospholipid molecule.

A

. Condensation (reaction) OR Loss of water;
. Between of glycerol and fatty acid;

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12
Q

Some seeds contain lipids. Describe how you could use the emulsion test to show that a seed contains lipids. (3)

A

. Crush/grind;
. With ethanol/ alcohol, to dissolve the lipid;
. Then add water then shake;
. Forms a white emulsion / goes white;

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13
Q

What are the differences between a triglyceride and a phospholipid?

A

. Fatty acid removed;
. Replaced with a phosphate group;

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14
Q

Compare and contrast the structure and properties of triglycerides and phospholipids

A

. Both contain ester bonds
. Both contain glycerol
. Fatty acids on both may be saturated or unsaturated
. Both are insoluble in water
. Both contain C, H and O but phospholipids are also contain P
. Triglyceride has three fatty acids and phospholipids have two fatty acids plus phosphate group
. Triglycerides are hydrophobic/non-polar and phospholipids have hydrophilic and hydrophobic region
. Phospholipids form monolayer/micelle/bilayer but triglycerides don’t.

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15
Q

Describe the biochemical tests you would use to confirm the presence of lipid, non-reducing sugar and amylase in a sample.

A

Lipid
1. Add ethanol/alcohol then add water and shake/mix OR Add ethanol/alcohol and shake/mix then pour into/add water;
2. White/milky emulsion OR emulsion test turns white/milky;

Non-reducing sugar
3. Do Benedict’s test and stays blue/negative;
4. Boil with acid then neutralise with alkali;
5. Heat with Benedict’s and becomes red/orange (precipitate);

Amylase
6. Add biuret (reagent) and becomes purple/violet/mauve/lilac;
7. Add starch, (leave for a time), test for reducing sugar/absence of starch;

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16
Q

A student carried out the Benedict’s test. Suggest a method, other than using a colorimeter, that this student could use to measure the quantity of reducing sugar in a solution.

A

. Filter and dry (the precipitate);
. Find mass/weight;

17
Q

Describe how you would produce a calibration curve for a reducing sugar of unknown concentration and use it to obtain results.

A

. Make up several known concentrations of maltose / glucose / lactose / galactose / fructose;
. Carry out the Benedict’s test on each sample;
. Use a calorimeter to measure the colour intensity of each solution and plot a calibration curve;
. Known conc on x-axis and absorbance/transmission on y-axis;
. Find the concentration of the unknown sample by using the calibration curve