Carbohydrates 3 Flashcards
How many molecules of G-3-P enter the payoff phase?
2
How many ATP are used in the prepatory phase?
2
How many ATP are made during the payoff phase?
4
What is the first reaction of glycolysis?
Phosphorylation of glucose
How many ATP is used during the first stage of glycolysis? (phosphorylation of glucose) And what enzyme is used?
1 ATP
Hexokinase
What is the second stage of glycolysis? And what is the enzyme?
Conversion of G - 6 - P to F-6-P
Phosphohexose Isomerase
Why does the second step of glycolysis proceed in both directions?
Reaction has a low free energy
What is the stage of glycolysis apart from step 1 (phosphorylation of glucose) that requires ATP?
Phosphorylation of F-6-P to F-1,6 - bisP (stage 3)
What is the catalyst for phosphorylation of F-6-P?
Phosphofructokinase -1
What is the splitting part of Glycolysis?
Step 4 - which is the cleavage F-1, 6-bisP, forms two different triose sugars
Catalyst is aldolase
What is stage 5 of glycolysis?
Interconversion of triose sugars
Enzyme used it triose phosphate isomerase
Dihydroxyacetone phosphate is converted to glyceraldehyde -3-phosphate
Forming 2 molecules of G-3-P
G-3-P is the only molecule that can enter the payoff phase
Which stage in glycolysis produces 2 molecules of NADH?
Stage 6, the oxidation of G-3-P to 1,3-bisPG
The catalyst is glyceraldehyde - 3 - phosphate dehydrogenase
The first payoff reaction in glycolysis
Reaction 6 (the oxidation of G-3-P to 1,3-bisPG) is coupled to which other reaction and why?
6 is coupled to 7 because 6 requires a lot of energy whilst 7 gives off a a lot of energy.
Describe reaction 7 and what is the substrate?
P transfer from 1,3-bisPhosphoglycerate to ADP, 2 ATP produced, phosphoglycerate kinase enzyme used
Spontaneous since highly exergonic
What is stage 8?
Conversion of 3, phosphoglycerate to 2, phosphoglycerate
Enzyme is phosphoglycerate mutase
