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ClinChem Lab 2ndYR 2ndSem Midterms > Carbohydrates > Flashcards

Carbohydrates Flashcards

1
Q

Possible Samples for Carbohydrates

A

Whole blood
Plasma
Serum
CSF
Pleural Fluid
Urine

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2
Q

Carbohydrate sample not used for Diabetic Diagnosis

A

Urine

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3
Q

How long can serum be stable at 25 deg C

A

8hrs

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4
Q

How long can serum be stable at 4 deg C

A

72 hrs

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5
Q

2 types of Glucose Methodologies

A

Chemical Method
Enzymatic Method

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6
Q

2 Types of Chemical Method

A

Oxidation Reduction Method
Condensation Method

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7
Q

Reduction of cupric ions to cuprous ions forming cuprous oxide in hot alkaline solution by glucose

A

Alkaline Copper Reduction Method

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8
Q

4 methods in Alkaline Copper Reduction Method

A

Folin Wu
Nelson Somogyi
Neocuproine
Benedict’s

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9
Q

Stabilizing agent for Benedict’s method

A

Citrate or Tartate

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10
Q

Involves reduction of a yellow ferricyanide to a colorless ferrocyanide by glucose

A

Hagedom Jensen

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11
Q

2 types Oxidative Reduction Method

A

Alkaline Copper Reduction Method
Alkaline Ferric Reduction Method

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12
Q

Under Condensation Method

A

Dubowski method

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13
Q

Dubowski Method will yield a what colored compound

A

green

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14
Q

2 types of glucose oxidase method

A

Colorimetric Glucose Oxidase
Polarographic Glucose Oxidase

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15
Q

Most specific Glucose Method

A

Hexokinase Method

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16
Q

Dextrostics are also called

A

Cellular Strip

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17
Q

Random Plasma Glucose was formerly known as

A

Random Blood Sugar

18
Q

Fasting Plasma Glucose was formerly known as

A

Fasting Blood Sugar

19
Q

Fasting Plasma Glucose specimens are collected after when

A

8-10hrs fasting

20
Q

When is fasting plasma glucose specimen ideally collected

A

Morning

21
Q

Specimens for Oral Glucose Tolerance Test

A

Urine and Blood

22
Q

OGTT fasting time

A

8-10hrs

23
Q

Different types of loads for OGTT

A
  1. 50grams (base line & 1st hour) (2x collection)
  2. 75 grams (base line up to 2nd hr) (3x collection
  3. 100grams (base line up to 3rd hr) (4x collection)
24
Q

HbA1c is also called as

A

Glycated Hemoglobin

25
Q

Normal value for Glycated Hemoglobin

A

4-6%

26
Q

Specimen Requirement for Glycated Hemoglobin

A

Serum
Plasma
Whole blood

27
Q

Anticoagulant of choice of HbA1c

A

Citrate
Heparin
Sodium Fluoride
EDTA

28
Q

Most widely used to assess short term glycemic control

A

Fructosamine

29
Q

What does fructosamine measure

A

Glucose that binds to the protein

30
Q

A test to detect very small levels of protein (albumin) in urine

A

Urine Microalbumin

31
Q

Test to detect Type 1 Diabetes Mellitus

A

Ketone Testing

32
Q

Ketone Testing Methods

A

Gerhardt’s
Sodium Nitroprusside
3-hydroxybutyrate dehydrogenase

33
Q

Diagnostic Criteria for Diabetes Mellitus:

Random Plasma Glucose level

A

greater than 200mg/dL

34
Q

Diagnostic Criteria for Diabetes Mellitus:

Fasting Plasma Glucose level

A

greater than 126mg/dL

35
Q

Diagnostic Criteria for Diabetes Mellitus:

2 hour Plasma Glucose OGTT level

A

greater than 200mg/dL

36
Q

Diagnostic Criteria for Diabetes Mellitus:

Gycated Hemoglobin level

A

greater than 6.5%

37
Q

Alkaline Copper Reductive:

Cuprous Ions + Phosphomolybdate

A

Folin Wu method

38
Q

Alkaline Copper Reductive:

Cuprous Ions + Arsenomolybdate

A

Nelson Somogyi

39
Q

7 Laboratory Tests for Glucose

A

Random Plasma Glucose
Fasting Plasma Glucose
Tolerance Test
HbA1c
Fructosamine
Urine Microalbumin
Ketone Testing

40
Q

5 Enzymatic Methods

A

Glucose Oxidase Method
Hexokinase Method
Glucose Dehydrogenase Method
Dextrostics
Interstitial Glucose Measuring Device

41
Q

Cuprous Ions + Phosphomolybdate -> ?

A

Phosphomolybdic Acid

42
Q

Cuprous Ions + Neocuproine -> ?

A

Cuprous-Neocuproine Complex

ClinChem Lab 2ndYR 2ndSem Midterms (2 decks)

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