Carbohydrates Flashcards
What is a monosaccharide?
A single molecular sugar unit
What are three examples of monosaccharides?
- Glucose
- Fructose
- Galactose
Draw the structures of alpha and beta glucose and highlight how they differ
https://homework.study.com/cimages/multimages/16/glucose7545349468638727623.png
The OH and H groups are swapped
Describe the test for a reducing sugar
Benedict’s test
- add sample
- add benedict’s reagent and warm in a water bath
- colour change from blue to red/orange/yellow/green (depending on concentration)
What type of test is the Benedict’s Test?
Semi-quantitative
How could you get a less subjective result, when doing the Benedict’s test?
Filter the ppt, dry and then find the mass
OR use a colorimeter and calibration curve
What is the bond between two monosaccharides called and how is it formed?
Glycosidic bond, formed by a condensation reaction
What are disaccharides?
Two monosaccharides joined together
What are 3 examples of disaccharides?
maltose (2 x alpha glucose)
sucrose (alpha glucose and fructose)
lactose (alpha glucose and galactose)
How would you test for a non-reducing sugar?
- add HCl and boil (this will hydrolyse any disaccharides present into monosaccharides)
- add NaHCO3 to neutralise
- add Benedict’s reagent and heat gently in a water bath
- colour change from blue to red/orange/yellow/green (depending on concentration)
How do you test for starch?
- add iodine solution and shake
- starch-> blue-black colour
How is starch suited for it’s function?
How does glycogen differ from starch?
It has shorter chains and is more highly branched
How is the structure of cellulose suited to its function?
BETA GLUCOSE- so form straight, unbranched chains
CHAINS RUN PARALLEL- cross-linked by H bonds which add collective strength
MICROFIBRILS- add more strength
What 2 types of polysaccharides can make up starch?
amylose and amylopecitin