Carbohydrate Metabolism Flashcards
To be a carbohydrate must have ___+ carbons
3
What is the formula for carbohydrate?
Cn(H2O)n
What are the smallest monosaccharides?
aldo and keto trioses (named for the placement of O double bond)
_____ is a epimer of glucose
galactose
what is a epimer?
each of two isomers with different configurations of atoms around one of several asymmetric carbon atoms present.
Lactose is made of what?
galactose and glucose (B 1,4 bond)
What happens to monosaccharides with greater than 5 carbons in aqueous solutions?
they cyclize
WHat is the difference between an a and b isomer?
A is below and B is above the plane
What kind of bond is on amylopectin?
A 1,4
A 1,6
(A 1,6 allows branching)
Sucrose is made of what?
glucose and fructose (A 1,2 bond)
What bond is present on a starch?
A 1,4
What enzymes break down amylopectin?
amylase and isomaltose
Why is cellulose indigestible?
because we do not have the enzyme needed to break down the B1,4 linkage
The glucose in oral dextran is derived from _____
sucrose (only get cavities from sucrose cause it donates its glucose for a slow growing glucose chain (streptococcal mutagens allows this))
What is cellulose?
most abundant carb in the world, indigestible
Humans lack the enzymes to degrade to ________ linkage
B 1,4 linkage
What are the health benefits of dietary fiber?
reduces risk for colon cancer, cardiovascular disease
What is soluble dietary fiber?
dissolved in water and forms a gel substance that is broken down by gut bacteria
What is insoluble dietary fiber?
does not dissolve in water, passes intact
What is insoluble fiber good for?
increases frequency of bowl movements
What is soluble fiber good for?
slows absorption, lowers glycemic index, lowers cholesterol
What type of carbohydrate can be taken up by the GI tract?
monosaccharides (bigger must be broken down)
where is disaccharidases found
brush boarder of SI
Salivary amylase breaks down ______ into _____
starch into a-dextrins (breaks the a 1,4 link)
_______ in the small intestine are brakes disaccharides down into _____
disaccarideases
Pancreatic amylase breaks down ____ ____ ____.
a- dextrin’s
trisaccharide’s
What do the glucose transporters of the blood transport?
GLUT 2
glucose, fructose, galactose
What are the glucose transporters in lumen? What do they transport?
SGLT 1 (active transport of glucose and galactose)
GLUT 5 (passive of fructose)
GLUTs transport goes with or against concentration gradient?
with
What transporter transports into tissues?
GLUT
SGLT transport goes with or against concentration gradient?
against, coupled to Na which is favorable
GLUT 2 receptors are found in which TISSUES?
liver, pancreas, small intestine
glucose sensor (Kt, which is similar to Km for glucose> blood glucose)
Rates of ____ transport depend on ____
_____ and _____ follows the second blank above
rates of glucose transport depends on Na concentration and glucose (coupled)
Chloride and water follow the Na absorbed
Sucrose is a _________________ for oral dextran
glucose donor
Where are glut 4 receptors found, what stimulates them?
in adipose, muscle, heart
directly stimulated by insulin
carbohydrates are polyhydroxy- _____
aldehydes or ketones
where are disaccharides broken down to monosaccharides?
small intestine
How does insulin increase glucose uptake?
increasing the amount of GLUT4 transporters at the cell surface
Insulin directly promotes________________ ______________________
glucose uptake by the tissues in adipose, skeletal heart muscle
for glycogen synthesis _____ must be converted to _____
G6P to G1P (by isomerase)
What is the structure of glycogen?
polymer of glucose with a 1,4 glycosidic bonds and a 1,6 bonds at branches
What is the difference in usage of glycogen in skeletal muscle vs liver?
muscle: fuel source for ATP generation in muscle itself
Liver: source of glucose for other tissues
What can G6P be used for
-glycolysis
-glycogen synthesis
-enter pentose phosphate pathway
What does G 1 P to UDP glucose do for glycogen synthesis?
donates glucose
What are glycogen degradation products
G1P and glucose
What does UDP glucose do?
donates glucose for syn of glycogen
What is the rate limiting step of glycogenesis?
glycogen synthase step (forms a a1,4 bond (cant form 1,6, branching enzyme does this))
What two enzymes are needed for glycogen synthesis?
branching enzyme
glycogen synthase (RLS)
What elongates the glycogenin primer?
glycogen synthase
Synthesis of glycogen requires what?
glycogenin primer and UTP glucose
(catalyzes the transfer of glucose from UDP-glucose to the OH group of Try194. glycogenin usually adds 7 more residues to the primer, glycogen synthase then elongates the glycogenin primer)
What is the rate limiting step for glycogenolysis?
glycogen phosphorylase
how does glycogen phosphorylase work?
usues inorganic phosphate to cleave the a1,4 bond and release G1P (major product, but CANT break a1,6 bond)
How is liver glycogen metabolism regulated in the muscle, in the fasting state?
stress and exercise favor muscle glycogenolysis
-epinephrine inhibits glycogen synthase
-Ca, AMP, epinephrine: activate glycogen phosphatase
How does debranching enzyme work?
uss H2O to cleave the a1,6 glycosidic bond and release free glucose (minor product cause not many 1,6)
How is liver glycogen metabolism regulated, in the liver, in the fed state?
INSULIN: favors glycogen synthesis,
activates glycogen synthase, inhibits glycogen phosphorylase
How is liver glycogen metabolism regulated in the liver, in the fasting state?
GLUCAGON/EPINEPRINE
favors glycogenolysis, inhibits glycogen synthase, activates glycogen phosphorylase
How is liver glycogen metabolism regulated in the muscle, in the FED state?
INSULIN: favors glycogen synthesis,
activates glycogen synthase, inhibits glycogen phosphorylase (SAME AS LIVER)
What are the steps of muscle glycogen phosphorylase during exercise?
- amp activates glycogen phosphatase
- Ca calmodulin activates phosphorylase kinase
3.epinephrine activates cAMP/PKA; PKA activates phosphorylase kinase
4.phospoylase kinase activates glycogen phosphorylase
Glycogen is the animal storage form of glucose with glucose
linked together in _____ and _____ glycosidic bonds
a-1,4- and a-1,6
Glycogen synthesis requires:
a primer (glycogenin), UDP-glucose, glycogen synthase (rate-limiting step) and branching enzyme.